Objective To observe the effects of paclitaxel,mitomycin,5-FU and cisplatin on the proliferation of tongue carcinoma cells and to guide the reasonable using of chemotherapeutic drugs and avoids the side-effects.Methods The fresh tongue carcinoma tissues from surgery were collected and unicellular suspension were prepared.Then paclitaxel,mitomycin,5-FU and cisplatin were used to investigate the drug sensitivity on primary tongue carcinoma cells and a blank control group was set up.The inhibitory effects of chemotherapeutic drugs on the growth of tongue carcinoma cells and the cell cycle phases were observed by MTT colorimetry and flow cytometry.Results The inhibitory rates of paclitaxel,mitomycin and 5-FU on tongue carcinoma cell proliferation were 45.3%,37.3%,and 36.2%,respectively,and in control group it was 2.1%,the difference was significant(P

Objective:To investigate the expression of P33~(ING1) protein in oral squamous cell carcinoma(OSCC) and its clinical significance. Methods:All samples of normal and cancer tissues from 30 patients with OSCC were examined for protein expression of P33~(ING1) by SP immunohistochemical staining.Results:The positive rate of P33ING1 in OSCC was 40%, that in normal oral mucosa tissue 100% (P

This article is to review the basic principles of renal perfusion in CT and MR,imaging method and perfusion characteristics in kidney diseases.It shows that in addition to the morphologic information,perfusion imaging can also provide functional and metabolic information about the renal neoplasms,which is very helpful for not only the differential diagnosis,but also the evaluation of tumor biological behaviors,it will have a bright future in clinical application.

Objective: To evaluate the electron beam computed tomography(EBCT) three-dimensional reconstruction in the diagnosis of bowel obstruction.Methods: Seventy-eight patients suspected of bowel obstruction underwent EBCT with two-phase enhancement scanning and 32 of them were re-established by corona-MPR and sagittal-MPR.All the EBCT results were analyzed and compared with the clinical findings.Results: Seventy-three of the cases were diagnosed as bowel obstruction by EBCT,which were all confirmed by surgery or follow-up,with an accuracy rate of 100% for location diagnosis and 86.3% for etiology.Conclusion: EBCT 3D-reconstruction has great advantages in determining the location,etiology and blood-supply of bowel obstruction.

Objective To investigate the in vitro effect of multi glycosidorum triptery (MGT) on cytokine production by splenocytes of oxazolone (OXZ) induced colitis in murine model. Methods Six mg of OXZ (in 50% of ethanol) was administered in male SJL/J mice intrarectally to induce colitis and mice were sacrificed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0.1 mg/ml or 0.01 mg/ml was added to the culture medium of splenocytes. Production of IFN ? and interleukin 4 (IL 4) in the supernatant was measured by ELISA. Results The production of IFN ? was suppressed by both 0.01 mg/ml and 0.1 mg/ml of MGT [normal control ( 1.24 ? 0.13 ) pg/ml→(0.97?0.26) pg/ml→(0.87?0.18) pg/ml, P

Objective:To develop bifunctional anti-human lingual cancer/anti-VEGF antibody.Methods:Antigen of lingual cancer was prepared from Tca 8113 cells and that of VEGF from endothelial cells of umbilical cord vein.The hybridoma cells of anti-human ligual cancer/anti-VEGF were prepared by the technique of secondary hybridoma.The antibody was identified by ELISA. Results:The specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.Conclusion:The bifunctional antibody of anti-human lingual cancer/anti-VEGF has good targeting potential.

AimTo establish the model of adriamycin- induced nephritic syndrome in rats and its pathological change wasobserved. MethodsThe model of nephritic syndrome in rats was established by intravenous injection of adriamycin in the dose of 5mg · kg-1 and 3 mg · kg-1 respectively at the first day and 15 th day. The amount of urine protein was measured at the 1、2、3、4、6、8 and 12 th week and the serum biochemical pa rameters of TG, TCH, BUN, Cr, TP and ALB were detected at the 6 th and 12 th week. Results The urine protein and the serum biochemic parameters were increased significantly, except the TP and ALB, which were decreased. Pathological changes at the sixth week was similar to minimal changes in glomerulonephritis, and at the 12 th week, sclerosis of glomeruli of kidney could be found. ConclusionAfter first injection,the changes of kidney were like those inminimal change glomerulonephritis of human being. But after the second injection. The kidney had the changes of focal segmental glomeralosclerosis.

Objective To measure the serum levels of vitamin D,total immunoglobulin E (tIgE),interleukin-4 (IL-4) and IL-6 in patients with atopic dermatitis (AD),to evaluate the association between vitamin D and severity of AD,and to investigate the role of vitamin D in inflammatory and immunoregulatory processes during the occurrence of AD.Methods Peripheral blood samples were collected from 37 patients with AD (AD group) and 30 healthy controls (control group).The serum levels of vitamin D,tIgE,and IL-6 were measured by chemiluminescent sandwich enzyme immunoassay,and those of IL-4 by enzyme-linked immunosorbent assay.The severity of AD was assessed by the SCORing atopic dermatitis (SCORAD) score.The t test or Mann-Whitney U test was performed to assess the differences in vitamin D,tIgE,IL-4 and IL-6 levels between the AD group and control group,chi-square test to compare the proportion of patients with vitamin D deficiency,insufficiency and sufficiency,and Pearson's correlation analysis or Spearman's correlation analysis to evaluate the correlations between the SCORAD score and serum levels of vitamin D,tIgE,IL-4 and IL-6.Results Compared with the control group,the AD group showed significantly decreased serum levels of vitamin D (24.77 ± 9.29 vs.28.98 ± 6.87 μg/L,t =2.015,P =0.048),but significantly increased serum levels of tIgE (137.68 [37.59-414.53] vs.45.16 [14.56-112.12] IU/ml,Z =-3.399,P =0.001),IL-4 (8.86 ± 4.83 vs.4.78 ± 3.07 ng/L,t =4.147,P ＜ 0.001) and IL-6 (6.53 [3.99-15.30] vs.4.58[2.85-8.17] ng/L,Z =-2.173,P =0.030).Among patients with AD,the SCORAD score was negatively correlated with serum levels of vitamin D (r =-0.505,P =0.001),positively correlated with those of tIgE (r =0.531,P =0.001) and IL-4 (r =0.519,P =0.001),but uncorrelated with those of IL-6 (r =-0.139,P =0.411).There were significant differences in the proportion of patients with vitamin D deficiency,insufficiency and sufficiency between the AD group and control group (x2 =8.762,P =0.013).AD patients with vitamin D deficiency showed significantly increased serum levels of tIgE (2846.87 [319.02-7300.00] IU/ml) and IL-4 ([16.37-2.05] ng/L) compared with those with vitamin D insufficiency (110.07 [26.20-501.48] IU/ml,P =0.045;[8.28 ± 4.48] ng/L,P =0.011) and those with vitamin D sufficiency (123.93 [91.61-273.68] IU/ml,P =0.024;[8.00 ± 4.63] ng/L,P =0.041).In addition,serum levels of IL-6 were also higher in patients with vitamin D deficiency than in those with vitamin D sufficiency (15.10 [8.49-30.72] vs.6.22 [4.47-9.47] ng/L,P =0.011].Conclusions Vitamin D deficiency or insufficiency exists in patients with AD.Vitamin D deficiency is correlated with high serum levels of tIgE,IL-4 and IL-6,and the severity of AD is closely correlated with increased serum levels of tIgE and IL-6 as well as decreased serum levels of vitamin D.

Objective To study the structure and function changes of erythrocyte in hypercholesterolemia rabbits that treated with irradiation in auricle root by low level 650 nm laser.Methods Sixteen male rabbits were selected to establish the model animal of hypercholesterolemia,successful model rabbits were divided into 2 groups (model and treatment groups),6 rabbits per group,and 6 normal rabbits were selected as control group.650 nm laser of 100 mW were given in auricle root in rabbits of treatment group for 16 weeks (6 d one week,twice a day,30 min one time).Blood from ear vein was collected every 4 weeks to perform routine and biochemical blood examination,and one-drop blood test under microscope was used for blood accumulation observation.Results Total number of red blood cells of treatment group decreased,while model group showed an increasing trend than normal group (P＜0.05).Hemoglobin in three groups were lower than other two groups (P＞0.05).Hematocrit (HCT) in treatment group were lower than that in normal group,while HCT in model group were higher than that in other two groups and showed a higher aggregation degree also.Less than model group,treatment group showed less aggregation degree but with higher total bilirubin.Conclusions Low level laser of 650 nm showed an effect on total number of erythrocytes,HCT and red blood cells aggregation,high dose of laser might be injury for erythrocytes and lead to total bilirubin increasement.

BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells.OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation. RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.