1.Erythrocyte CD58 expression in healthy population undergoing moxibustion: An analysis of 40 cases
Leiyong LI ; Yuefeng TIAN ; Gehong WANG ; Jun WANG ; Binren ZHANG ; Xu WANG ; Yingxia MENG
Chinese Journal of Tissue Engineering Research 2009;13(49):9735-9738
BACKGROUND: CD58 is a glycoprotein, belongs to immune protein superfamily members, widely expresses in various human immunocytes and erythrocytes, and is the key immune substance of regulating T cell immune function by erythrocytes.OBJECTIVE: To explore the influence of moxibustion on the acupoints of Shenque (CV8) and Guanyuan (CV4) on erythrocyte CD58 expression in healthy people.DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at the Central Laboratory, Shanxi College of Traditional Chinese Medicine from March to December 2008.PARTICIPANTS: A total of 40 healthy undergraduates, 20 males and 20 females, aged mean 21.08 years, admitted in Shanxi College of Traditional Chinese Medicine were selected. Informed consents were obtained from each participant.METHODS: Putting the medicinal cakes on the acupoints, Shenque (CV8), Guanyuan (CV4), Zusanli (ST36), Pishu (BL20),Shenshu (BL21), and then lighted moxa every other day, totally for 10 times. Different quantities were compared before and after applying moxibustion.MAIN OUTCOME MEASURES: Hemocyte routine examination was performed before and after moxibustion. Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were determined by flow cytometry.RESULTS: The quantities of erythrocytes and leukocytes were increased significantly following moxibustion (P < 0.01). Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were significantly greater following moxibustion (P < 0.01), especially fluorescent strength of CD58 molecule on erythrocyte surface following moxibustion.CONCLUSION: There are close relationships between CD58 expression and moxibustion. Moxibustion with medicinal cake plays an important role in the immune function of a body.
2.Effects of Dredging Collaterals and Activating Blood Worm Chinese Materia Medica on Angiogenesis Related Factors of Lung Cancer in Hypoxic Environment
Daorui LI ; Miaomiao WANG ; Mingwei YU ; Fei LIN ; Ying ZHANG ; Meng LI ; Huiting FAN ; Qi ZHENG ; Xin QI ; Yingxia PEI ; Peitong ZHANG ; Wei HOU ; Hongsheng LIN
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(9):39-42
Objective To observe the effects of dredging collaterals and activating blood worm Chinese materia medica on angiogenesis related factors of lung cancer in hypoxic environment. Methods The lung cancer A549 cells were cultured in vitro to simulate tumor hypoxia microenvironment by the hypoxia workstation, and different concentrations of Scorpio, Scolopendra and Gecko medicated serum were added. MTT method was used to detect cell proliferation and screen the best medicine concentration and duration of action. Lung cancer A549 cells were administrated by the three kinds of medicated serum, and cells were collected and supernatant was cultured. Contents of VEGF, TGF-β1, and bFGF were detected by ELISA. Results Three kinds of medicated serum had the inhibitory effect on both added normoxia and hypoxia in cultured A549 lung cancer cells. 7.5% concentration of medicated serum was selected, and 24 h later were used in later experiments. Scorpio, Scolopendra and Gecko medicated serum can more reduce the contents of VEGF, TGF-β1 and bFGF in the supernatant of A549 cell compared with the control group (P<0.05, P<0.01). Conclusion Dredging collaterals and activating blood worm Chinese materia medica had inhibitory effect on cancer cells and the regulation of angiogenesis related cytokines in the condition of normoxia and hypoxia.