OBJECTIVE:To study the bioavailability of single oral dose of galanthamine hydrobromide(GH) sustained-release pellet capsule (GHSRPC) in healthy dogs. METHODS: By a two-cycle crossover trial, 6 dogs were randomly assigned to receive GHSRPC or GH capsules respectively by ig. Blood samples were taken at different time and the plasma concentrations of GHSRPC and GH capsules were determined by HPLC, meanwhile their pharmacokinetic parameters were computed. RESULTS: After a single dose administration of GHSRPC and GH capsules, the pharmacokinetic parameters were as follows: AUC were (1 867.24?321.05) and (1 604.60?289.17) ng?h?mL-1; tmax were (5.42?2.24) and (0.73?0.62) h; Cmax were (89.62?11.23) and (162.82?15.35) ng?mL-1; t1/2 were (8.93?1.82) and (7.58?1.21) h, respectively. The relative bioavailability of the GHSRPC was 116.36%. CONCLUSION: The results showed significant sustained release characteristics of the GHSRPC, It’s bioequivalent with GH capsule.

The gene of the caudal-type homeobox gene -2 ( CDX2), a member of the CDX family, is a principal regulator in the development of embryogenesis and haematogenesis . Recent studies have shown aberrant expression of CDX2 in each subtype of leukemia and its involvement in the genesis, progress and prognosis of leukemia as new gene. CDX2 may be a potential gene maker of leukemia in minimal residual disease detection.Studies also suggested that CDX2 be involved in leukemia by modulating HOX gene. To further investigate the mechanism of CDX2 in leukemia can be of great significance for the prediction of relapse and the individualized and targeted therapy.

AIM: To optimize the best technique of making Semen Euphorbiae into frost-like powder. METHODS: Machine was developed equipped with temperature and pressure control. Pretreatment of Semen Euphorbiae, heating temperature and time were selected as factors,fat oil content,euphorbiasteroid and lathyrol diadetate benzozte as main levels,to evaluate the processing. RESULTS: The experiment showed that seeds of Semen Euphorbia were directly broken into powder and heated at 55 ?C for 30 min. The oil productivity ranged from 51% to 53% . CONCLUSION: The heating temperature has an influence on fat oil and laxative constitutents' content, whereas pretreatment of seed and heating time lack the relationship to.

OBJECTIVE:To study the preparation and the quality control of the compound lomefloxacin nose-drops MET_HODS:The first order derivative spectrophotometry was used to determine the content of lomefloxacin with no need of isolation The detecting wavelengths were 293nm and 315nm RESULTS:The correlation coefficient was 0 9 999 The average recovery was 100 77%,with RSD of 0 82% CONCLUSION:The preparation process of compound lomefloxacin nose-drops was simple,the first order derivative spectrophotometry for the determination was rapid,simple and accurate

Chitosan and it's derivatives have good biologic and particular physical and chemical properties. They have a wide applied foreground on drug preparations. This study introduced the recent advances of them based on widely researching the information and the actual works, and provided reference for the study of oral drug preparations.

Objective To introduce and translate the Coping-adaptation Processing Scale:Short Form-15 items (CAPS:SF) and evaluate the reliability and validity among people after losing the only-child. Methods CAPS:SF was translated into Chinese (CV-CAPS:SF) according to Brislin. A sample of 77 people after losing the only-child were recruited to complete the scale to evaluate the reliability and validity of scale. Results The scale-content validity index of the CV-CAPS:SF was 0.94;the Cronbachαwas 0.85 and split-half coefficient was 0.84. The score of coping was (38.60 ± 5.30) points. The take strength from spirituality or the successes of courageous people was the highest (3.44±0.50) points;the use humor in handling the situation was the lowest (2.05±0.58) points. Different age, education degree, marital status, occupation, average monthly income of latest year, the length after losing the only-child in the face of the difference was statistically significant (t/F=7.636-85.130, all P<0.05). Conclusions The CV-CAPS:SF is reliable and valid. It could be used to help the nursing staff understand coping among people after losing the only-child .

Objective To invetigate the clinical effect of Guyouling Liniment in the treatment of soft tissue contusion.Methods Sixty-three cases of soft tissue contusion in our hospital from October 2012 to January 2014 were selected and divided into the treatment group (n=32)and control group(n=31).The treatment group were treated with Guyouling Liniment and the control group adopted Indomethacin Liniment.The effects were compared between the two groups.Results Twenty-six cases(81.13％) in the treatment group were cured and 30 cases (93.75％) were effective;23 cases(74.19％) in the control group were cured and 27 cases (87.10％) were effective.The clinical effect of the treatment group was better than that of the control group with statistical difference(P＜0.05).Compared with the control group,the pain degree and stasis purple area in the treatment group were significantly alleviated,the difference was statistically significant(P＜0.05).Conclusion Guyouling Liniment has significant effect in the treatment of soft tissue contusion and is worthy of clinical promotion.

Objective:To explore differential diagnosis value of changes of brain natriuretic peptide (BNP) and N ter‐minal pro brain natriuretic peptide (NT‐proBNP) concentrations in patients with dyspnea .Methods :A total of 150 patients with dyspnea at hospitalization were enrolled and divided into cardiogenic dyspnea group (CD group ,n=51) ,pulmonary dyspnea group (PD group ,n=47) and cardiogenic plus pulmonary dyspnea group (CPD group ,n=52) .Another 40 healthy subjects were regarded as normal control group .Concentrations of BNP and NT‐proBNP were observed and compared among all groups before and after treatment .Results:Before treatment ,compared with normal control group , there were significant rise in plasma concentrations of BNP and NT‐proBNP in CD group ,CPD group and PD group ,that of CD group was significantly higher than those of PD group and CPD group , and that of CPD group was significantly higher than that of PD group , P<0.01 all .Before treatment ,the NT‐proBNP content in CD group ,CPD group , PD group were [ (3356.6 ± 321.2) pg/ml vs . (3156.9 ± 239.8) pg/ml vs .(2563.7 ± 234.20) pg/ml] respectively , comparison among three groups , P<0.01 all .Compared with before treatment ,one week after discharge ,plasma concentrations of BNP and NT‐proBNP significantly reduced in CD group ,CPD group and PD group ,the plasma BNP and NT‐proBNP concentrations of CD group were the highest , those of PD group were the lowest ,and those of CPD group were the middle , P<0.01 all .Conclusion:Plasma BNP and NT‐proBNP concentrations are helpful to differential diagnose whether dyspnea belongs to cardiogenic or pul‐monary diseases .

AIM: To detect free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC. METHODS: A method for determining gallic acid in leaves of Acer Truncatum Bunge.The operating conditions HPLC were Diamonsil C_18 column(5 ?m,4.6 mm?250 mm) at room temperature,methanol-0.025 mol/L phosphoric acid solution(15∶85) as mobile phase at a flow of 1.0 mL/min and UV detection at 272 nm. RESULTS: The contents of free and hydrolysable gallic acid were 0.045% and 1.546%,repectively.The relative standard deviations of free and hydrolysable gallic acid were 1.06% and 2.43%,respectively. CONCLUSION: The contents of free and hydrolysable gallic acid in Acer Truncatum Bunge in different periods are different.