2.Expression of Maspin in invasive fungal rhinosinusitis
Hewei YU ; Zonghui KANG ; Dan PAN ; Siwen XIA ; Xianhui HU ; Yingwu CHEN ; Yunsheng HE ; Yideng HUANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(6):349-352
OBJECTIVE To explore the expression of Maspin in invasive fungal rhinosinusitis (IFRS) and the value of Maspin in the diagnosis of IFRS. METHODS Forty two cases of fungal rhinosinusitis (FRS) were set as the experimental group, which included 12 cases of IFRS and 30 cases of noninvasive fungal rhino-sinusitis (NIFRS). At the same time, 30 cases of chronic rhino-sinusitis were set as control group. Immunohistochemistry (IHC) was used to detect the expression of Maspin. RESULTS Compared with the control group, the expression of Maspin in FRS group decreased statistically (t=-3.367, P<0.05). The IFRS group, compared with other two groups, had the lowest expression of Maspin (t=-3.390, P<0.05; t=-4.143, P<0.05). By using Maspin score of 5.70 as the cut-off point, the sensitivity and specificity for the diagnosis of IFRS was 91.7% and 88.3% respectively. CONCLUSION The expression of Maspin is very low in IFRS group. Down-regulation of Maspin expression may be a potential indicator for diagnosis of IFRS.
3.Role of Ultrasound Elastography in Evaluation of Lymphedema and Rehabilitation for It (review)
Lin YUAN ; Sufang SHEN ; Yue LI ; Jing WU ; Yingwu CHEN ; Liping HUANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(9):1056-1059
Elastography can study the soft tissues by quantitative imaging of strain and elastic modulus distributions in soft tissues. For lymphedema, the strain profile of the soft tissues can be observed with the elastography to classify the lymphedema and evaluate the effect of rehabilitation.
4.Basal cell adenocarcinoma of nasal septum: a case report and review of literatures.
Jianwen ZHENG ; Yingwu CHEN ; Jinghua ZHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;23(5):209-210
OBJECTIVE:
To summarize clinical features, diagnosis, differential diagnosis, treatment and prognosis of basal cell adenocarcinoma.
METHOD:
A retrospective study were subjected to one case with basal cell adenocarcinoma of the nasal septum, and the related literature were reviewed.
RESULT:
Basal cell adenocarcinoma often occurred in the salivary glands and minor salivary glands of salivary palate and other parts. The symptoms mainly presented with a local lump. Major treatment method was radical surgical resection and, if necessary, supplemented by radiotherapy.
CONCLUSION
Basal cell adenocarcinoma is rare seen in the salivary gland tumors. It has a tendency of low-grade malignancy but need long-term follow-up of a large samples.
Adenocarcinoma
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radiotherapy
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surgery
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Aged
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Humans
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Male
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Nasal Septum
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pathology
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Nose Neoplasms
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radiotherapy
;
surgery
;
Retrospective Studies
5. VitalStim electrical stimulation combined with traditional swallowing therapy alleviates dysphagia among Parkinson′s patients
Jiasheng TAN ; Chong Leong LAO ; Yingwu CHEN
Chinese Journal of Physical Medicine and Rehabilitation 2019;41(12):928-931
Objective:
To investigate the effect of VitalStim electrical stimulation combined with traditional swallowing therapy on severe dysphagia in patients with Parkinson′s disease (PD).
Methods:
In all, 36 PD patients with severe dysphagia were randomly divided into a treatment group and a control group, each of 18. Both groups were given the traditional swallowing therapy, while the treatment group was additionally provided with VitalStim electrical stimulation. Before and 15 days after the intervention, both groups were assessed using the modified Mann assessment of swallowing ability (MMASA) and standardized swallowing assessment (SSA).
Results:
After the intervention the average SSA score of the control group had dropped significantly, but no significant change was observed in their average MMASA score. However, significant improvement was observed in both the average MMASA score and the average SSA score of the treatment group. Moreover, the cure rate of the treatment group was 47.1%, significantly higher than that of the control group.
Conclusion
VitalStim electrical stimulation combined with traditional swallowing therapy is effective for alleviating severe dysphagia among PD patients. It is worthy of application and further study in clinical practice.
6.Genetic transformation of Bt gene into sorghum (Sorghum bicolor L.) mediated by Agrobacterium tumefaciens.
Mingzhou ZHANG ; Qiao TANG ; Zonglun CHEN ; Jun LIU ; Hairui CUI ; Qingyao SHU ; Yingwu XIA ; I ALTOSAAR
Chinese Journal of Biotechnology 2009;25(3):418-423
Sorghum (Sorghum bicolor L.) was one of the most important crops in the world next to wheat, rice, maize, soybean and barley. Using the callus derived from immature inflorescence as the recipients, we efficiently transformed sorghum varieties 115, ICS21B and 5-27 with the insecticidal Bacillus thuringiensis (Bt) cry1Ab gene carried in the T-DNA of binary vectors which contained hygromycin resistance gene and gus gene via Agrobacterium tumefaciens. After gradient selection with hygromycin, a total of 21 independent transgenic plant lines, 52 transgenic plants were regenerated, and the average stably transformation efficiency was 1.9%. The integration and transcription of cry1Ab gene in transgenic sorghum was confirmed by PCR analysis, Southern blotting and RT-PCR analysis. The Bt proteins were expressed in most transgenic plants with different level from plant to plant by Western blotting and ELISA assay. According to insect bioassay in laboratory, the transgenic plants with a relatively high level of Bt gene expression displayed insect-resistance to pink rice borer (Sesamina inferens).
Agrobacterium tumefaciens
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genetics
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metabolism
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Bacterial Proteins
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genetics
;
metabolism
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Endotoxins
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genetics
;
metabolism
;
Hemolysin Proteins
;
genetics
;
metabolism
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Pest Control, Biological
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Plants, Genetically Modified
;
genetics
;
Sorghum
;
genetics
;
Transformation, Genetic
7.Prognostic value of AST/ALT ratio combined with APTT regimen in NVAF patients receiving rivaroxaban anticoagulant therapy
Yi CHEN ; Meng NING ; Yingwu LIU
International Journal of Biomedical Engineering 2023;46(2):138-143
Objective:To investigate the prognostic value of the ratio of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) combined with activated partial thromboplastin time (APTT) in elderly patients with non-valvular atrial fibrillation (NVAF) treated with rivaroxaban.Methods:One hundred and twenty-two elderly patients with NVAF who were anticoagulated with rivaroxaban from June 2020 to June 2021 in the Third Central Hospital of Tianjin were enrolled and divided into four groups based on the median method. The patients in the Q1 group ( n = 32) have low AST/ALT/low APTT. The patients in the Q2 group ( n = 27) have low AST/ALT/high APTT. The patients in the Q3 group ( n = 29) have high AST/ALT/low APTT. The patients in the Q4 group ( n = 34) have high AST/ALT/high APTT. The efficacy endpoint events, and safety endpoint events were analyzed in the four groups, and univariate and multivariate Cox regression analyses were performed for the composite endpoint events. Results:The effectiveness endpoint events were mainly cardiovascular deaths, the number of which in the Q1 to Q4 groups was 0 (0), 1 (3.70%), 4 (13.79%), and 5 (14.71%), respectively. The safety endpoint events were mainly non-major bleeding events, the number of which in the Q1 to Q4 groups was 5 (15.62%), 2 (7.41%), 6 (20.69%), and 5 (14.71%), respectively. Compared to the Q1 group, the Q4 group had an increased risk of composite endpoint events after incorporating traditional risk factor correction ( HR: 3.851, 95% CI: 1.167 to 12.704). Conclusions:AST/ALT ratio combined with APTT can provide risk stratification for distant bleeding and cardiovascular adverse events in elderly NVAF patients treated with rivaroxaban anticoagulation and has some predictive value for their prognosis.
8.Study on intestinal flora characteristics of asthmatic children based on theory of lung and large intestine being interior-exterior
Jia CHEN ; Yingwu CHE ; Jie LIN ; Yiqin WANG ; Kexing SUN ; Fang LIU
International Journal of Traditional Chinese Medicine 2021;43(8):744-750
Objective:To explore the characteristics of intestinal flora in children with asthma from the theory of "lung and large intestine being interior-exterior" , and to provide an experimental basis for the study of "lung and intestine axis" .Methods:Based on 16S rDNA high-throughput sequencing technology, stool samples from children with 30 asthma and 28 healthy children were enrolloed and sequenced. All patients are from january 2018 to April 2018, Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical College and Oriental Hospital Affiliated to Tongji University. Statistical methods such as Principal component analysis (PCA), LEfSe analysis, linear discriminant analysis (LDA), and non-metric multidimensional scale analysis (NMDS) were used to analyze intestinal flora characteristics of asthma children.Results:The number of Intestinal flora operating taxon unit (OTU) in asthmatic group (141.96 ± 27.42 vs. 164.54 ± 50.58, P=0.048), Shannon index (2.49 ± 0.51 vs. 2.80 ± 0.56, P=0.044) were significantly lower than those of healthy children group, and Simpson index (0.18 ± 0.06 vs. 0.13 ± 0.07, P=0.010) was significantly higher than that of healthy children. There was no significant difference in intestinal flora abundance between asthmatic children and healthy children ( P>0.05), but there was a decreasing trend. Further differential intestinal flora analysis showed that there were differences in bacterial abundance between children with asthma and healthy children. Conclusion:Children with asthma showed high related with intestinal flora disorders, referring that asthma maybe treated when intestinal flora is treated, which provides a reference for the study of the relationship between lung and intestine from the perspective of intestinal flora.