One hybridoma cell line (2G8) secreting monoclonal antibody against humn allotype G2m (23 )was produced by fusion of myeloma cell (SP2/0) and spleen cells from BALB/c mice immunized withlgG2 [G2m (23 + )], isolated and purified with FPLC protein A column from human plasma. Themonospecificity of McAb against G2m (23 ) antigen was testified by ELISA-inhibition, Sandwich -ELISA and Dot-ELISA test.

BALB/C mice were immunized by intraperitoneal injection with A-group erythrocytes, B-group erythrocytes and saliva of A-group,B-group secretor. Immune spleen cells were fused with sp2/0 murine myeloma cells. The supernatants from each well were examined for antibodies by hemagglutination using A-,B-, O-group RBC.3 hybridoma cell lines of secreting anti-A antibodies and 3 hybridoma cell lines of secreting anti-B antibodies were established.These hybridoma cells have maintained and produced antibodies possessing higher specificity and titer.The agglutination titer of supernatants reached 2048-fold. These antibodies were used in the field of forensic science.