Objective To investigate the protective effect of gross saponin tribulus terrestris(GSTT) on the apoptosis of pheochromocytoma cells(PC12 cells)induced by H2O2 and its mechanisms.Methods PC12 cells were divided into control,model,high dose GSTT(GSTT1) and low dose GSTT(GSTT2) groups.Apoptosis of PC12 cells was induced by H2O2 at the concentration of 300? mmol?L-1.The cell activity was determined by MTT.The subdiploid peaks showing cell apoptosis rate and ?m were detected by flow cytometry.Proteins of Bcl-2 and Bax were detected by immunohistochemistry.Results Compared with model group,the survival rate of PC12 increased(P

Aim To investigate the protective effect of gross saponin tribulus terrestris(GSTT) on the apoptosis of PC12 cells was induced by H2O2 and its mechanism.Methods Apoptosis of PC12 cells was induced by H2O2 at the concentration of 300 ?mol?L-1.The cell activity was determined by MTT.Cells were observed by inverted phase contrast microscope.Hochest33258 staining was detected by fluorescence microscope.The subdiploid peaks showing cell apoptosis rate was detected by flow cytometry.Protein of Bcl-2 and Bax was detected by Western blot.Results Compared with model group,the survival rate of PC12 cells increased (P

[ Objective ] To investigate the mechanism of Ningxian Granule (NG), a compound prescription with lucidity-ascending and turbidity-descending actions. [ Methods ] Forty SD rats were randomized into 5 groups: A (normal), B (model), C (low-dose NG, 5 g/kg), D (high-dose NG, 10 g/kg) and E (phenobarbital, 60 mg/ kg). Except Group A, the others were epileptic rats induced by pentylennetetrazol (PTZ, 35 mg/kg). NG and phenobarbiatal were given to different groups accordingly, once a day for 4 weeks. The effects of NG on brain-derived neurotrophic factor (BDNF) protein expression was observed by using immunohistochemistry technique. [Results] The number of positive neuron increased both in the hippocampus and cerebral cortex of epileptic rat brain (P