ObjectiveTo investigate the quality of life in patients with ankylosing spondylit2s(AS). Method86 patients were investigated by direct questioning using a uniform questionaire. ResultAll patients had different degree of stiffness on the lower back. 77.9% and 75.8% ofpatients ftequently had pain and fatigue respectively. 24. 4% of patients had sleeping disorder. 39.5% of patients had to change job or could notundertake normal work. 86% of patients had limitations in participating social activities. irritable feelings developed in 94. 1% of patients.91.8% of patients were not regularly treated before being admitted . ConclusionAll patients had few imitation in self- care and elementaryactivities in self - care. Most patients could perform their house hold tasks, although their severe symptoms have periodically flares. Many patientshad psychological problems.

Objective: To understand the trends in malnutrition among primary and middle school students of Han nationality in Hainan Province from 2005 to 2019, so as to provide a basis for improving nutrition intervention measures for children and adolescents. Methods: A sample of 32 949 Han nationality primary and middle school students aged 7-18 years old in Hainan Province were investigated in 2005, 2010, 2014 and 2019 based on national survey on student physical fitness and health. The Malnutrition Screening Standard of Schoolage Children and Adolescents was used to screen malnutrition. Statistical analysis was performed using the χ2 test and the χ2trend test. Results: In the four surveys conducted during 2005 to 2019, the prevalence of malnutrition among primary and middle school students were 22.12%, 18.80%, 15.89% and 9.56%, respectively, with an increase of -12.56% and an average annual increase of -5.82%. The decreasing trend of malnutrition by year was statistically significant (χ2trend=600.72, P<0.01), and the proportion of emaciation type was the highest (8.87%-20.15%). The detection rates of malnutrition among all students aged 7 to 18 showed a decreasing trend from 2005 to 2019 (χ2trend=56.44, 60.04, 61.48, 42.49, 51.81, 50.81, 72.86, 101.34, 86.38, 24.81, 17.72, 10.38, P<0.01). From 2005 to 2019, the detection rates of malnutrition in boys were higher than that of girls (in 4 surveys), and that in rural students from 2005 to 2014 of 3 surveys were higher than that in towns (χ2=92.07, 35.16, 25.29, 29.98; 64.35, 4.26, 6.32, P<0.05). Conclusions The malnutrition of Han nationality primary and middle school students aged 7-18 years in Hainan Province show a trend of improvement year by year from 2005 to 2019, despite the overall high detection rate. Wasting is the most common type of malnutrition. The epidemic of malnutrition varies by age, sex and areas. Further targeted measures should be taken to strengthen intervention in the diet of primary and middle school students, to improve the nutritional status of children and adolescents.

Objective:To investigate the changes in the hypothalamic metabolites of lipopolysaccharide(LPS)febrile young rabbits after the treatment with pediatric tuina.Methods:A total of 30 young rabbits were randomly assigned into three groups:the normal group,the model group,and the tuina group.Both the model group and the tuina group were injected intrave-nously with LPS."Six antipyretic manipulations"(pushing Tianmen,pushing Kangong,kneading Taiyang,kneading Erhougaogu,clearing Tianheshui,and pushing Jizhu)were administered 1 h after the LPS in-jection in the tuina group.The rectal temperatures of the young rabbits were monitored during the experiment to explore the antipyretic effect.Three hours after the injection,the content of interleukin-1 β(IL-1β),tumor necrosis factor(TNF)-α,and prostaglandin E2(PGE2)in the serum was detected.In addition,liquid chromatography-mass spectrometry(LC-MS)was used for the hypothalamus metabolomics.Results:Compared with the model group,the rectal temperature of the tuina group was decreased at 2 h and 3 h after the LPS injection(P=.04,P=.03,respectively),and the content of IL-1β,TNF-α,and PGE2 was decreased(P=.03,P=.003,and P=.008,respectively).The metabolomics results showed that there were 23 potential biomarkers after the tuina intervention,enriching 27 pathways.Lipid metabolites,especially glycerophospholipids,were a majority of the altered metabolites.The primary metabolic pathways affected by tuina included the arachidonic acid metabolism,the GABAergic synapse,D-gluta-mine and D-glutamate metabolism,and the glutamatergic synapse.Conclusion:Pediatric tuina reduced the temperature of the febrile rabbits and downregulated the expression of IL-1β,TNF-α,and PGE2,and the antipyretic mechanism may be related to changes in metabolites and metabolic pathways in the hypothalamus.

Objective By observing the effects of"three methods and three points"tuina technique on the expression of interleukin-17F(IL-17F),interleukin-17 receptor C(IL-17RC),activator 1 of nuclear transcription factor-κB(Act1),tumour necrosis factor receptor-associated factor 6(TRAF6)and M1 microglial cell expression in the spinal dorsal horn of rats with mild chronic compressive injury(minor CCI)model,we explored the immediate analgesic mechanism of tuina on peripheral neuropathic pain(pNP).Methods Thirty-six SD rats were divided into the sham group,the model group and the tuina group according to the random number method,twelve rats in each group,and the minor CCI model was replicated by ligating the right sciatic nerve.The rats in the tuina group were subjected to pointing,plucking and kneading at the BL37,BL57 and GB34 points on the affected side using a tuina simulator,while the sham group and the model group were only grasped and restrained,and were intervened for one time.The mechanical pain test and cold plate test were used to evaluate the response of rats to mechanical stimulation and cold stimulation after immediate intervention.The protein expression of IL-17F and TRAF6 in the spinal dorsal horn of rats in each group was detected by Western blotting.The mRNA expression of IL-17F,IL-17RC,Act1 and TRAF6 in the spinal dorsal horn of rats in each group was detected by real-time PCR.The average fluorescence intensity of M1 microglia in the spinal dorsal horn of rats in each group was detected by immunofluorescence.Results Behavioral results showed that before intervention,compared with the sham group,paw mechanical withdraw threshold(PMWT)decreased and cold sensitivity threshold(CST)increased in the model group and the tuina group;after tuina intervention,PMWT in the tuina group was increased,and CST was decreased compared with the model group;after intervention,PMWT in the tuina group was increased,while CST was decreased(P＜0.05).RT-PCR results showed that compared with the sham group,mRNA expression levels of IL-17F,IL-17RC,TRAF6 and Act1 in the spinal dorsal horn of the model group were increased;compared with model group,the mRNA expression levels of above indexes in the tuina group were decreased(P＜0.05).Western boltting results showed that compared with the sham group,the expression levels of IL-17F and TRAF6 protein in the spinal dorsal horn of the model group were increased;compared with the model group,the expression levels of IL-17F and TRAF6 protein in the tuina group decreased(P＜O.05).Immunofluorescence results showed that the mean fluorescence intensity of CD40 in the spinal dorsal horn of model group was enhanced compared with the sham group;compared with the model group,the mean fluorescence intensity of CD40 in the tuina group was decreased(P＜0.05).Conclusion The"three methods and three points"tuina technique can produce immediate analgesia by inhibiting the expression of IL-17F,IL-17RC,Act1,TRAF6 and the activation of M1 microglia in the dorsal horn of the spinal cord after one intervention.

Rats ; Animals ; Sciatic Neuropathy ; Muscle, Skeletal ; Sciatic Nerve/physiology* ; Sequence Analysis, RNA ; Nerve Regeneration/physiology*