OBJECTIVE: To establish an HPLC method for the determination of Quercetin in Duizuoye tablet. METHODS: HPLC analysis was carried out on Dikma-ODS C18 column (250 mm?4.6 mm, 5 ?m) with methanol-0.4% phosphoric acid solution(53∶47) as mobile phase at a flow rate of 1.0 mL?min-1; the detection wavelength was set at 370 nm and the column temperature was maintained at 35 ℃. RESULTS: The calibration curve for Quercetin was linear in the range of 1.31～9.17 ?g?mL-1 (r=0.999 8).The average recovery was 98.06%, with RSD of 1.05% (n=6).CONCLUSION: The adopted method is simple, rapid and accurate, and suitable for the quality control of Duizuoye tablet.