Objective To investigate the effect of infrasound on the growth of bone marrow mescenchymal stem cells (BMSCs) and seek the most reasonable duration for using infrasound. Methods The primary BMSCs were obtained from Sprague-Dawley rats through whole bone marrow adherent cultivation.The cells of passage 3 were divided into trial groups treated with infrasound for 10 min,30 min or 60 min,and control groups which were not treated with infrasound but exposed to air for the same durations.The vitality of cell proliferation was measured using the CCK8 method.Apoptosis and the cell cycle were analysed with flow cytometry (FACS). Results After cultivation for 72 h,the optical density (OD) values for BMSCs treated with infrasound for 10 min,30 min and 60 min were 1.480 ± 0.030,1.348 ± 0.030,1.493 ± 0.030 respectively and after 96 h they were 1.774 ± 0.030,1.731 ± 0.030 and 1.833 ± 0.030.All of which were significantly greater than in the control groups ( 1.479 ± 0.030,1.267 ±0.030 and 1.227 ± 0.030 after 72 h and 1.567 ± 0.030,1.563 ± 0.030 and 1.632 ± 0.030 after 96 h).With the extension of the treatment duration,the OD values of the BMSCs increased or increased after decreasing,and the OD value for BMSCs treated with infrasound for 60 min was the highest.The FACS results indicated no effect of infrasound on apoptosis of BMSCs when the treatment duration was 30 min,but that cell apoptosis could be inhibited when the treatment duration was 10 min or 60 min.The early phase apoptosis rates were 1.07％ ± 0.12％ and 0.97％ ±0.21％ in the trial groups treated for 10 min and 60 min respectively,and 1.43％ ± 0.06％ and 3.33％ ± 0.15％ in the respective control groups,a highly significant difference.The results of cell cycle analysis showed infrasound could disturb the cell division of BMSCs significantly when the treatment duration was less than 30 min,but there was no significant effect when the treatment duration was 60 min. Conclusions Infrasound can promote cell proliferation and disturb cell division,but it did not cause apoptosis of BMSCs.When the treatment duration was 60 min,cell proliferation improved steadily,the apoptosis rate decreased,and the cell cycle did not change.So 60 min is the most reasonable treatment duration for infrasound.

Small cell carcinoma of cervix (SCCC) is a rare and high malignant neuroendocrine tumor.Because of its strong invasion,metastasis in early stage and easy recurrence,the prognosis is poor.Its diagnosis is based on pathomorphology,and immune-histochemistry biomarkers can improve its diagnosis.Combined therapy is recommended.Postoperative platinum/etoposide (PE),vincristine/adriamycin/cyclophosphamide (VAC) or taxel/carboplatin(TP) chemotherapy can improve the prognosis.

Objective To investigate the efficacy and side effects of MINE regimen, as a salvage regimen, in treating patients with recurrent and refractory intermediate grade non-Hodgkin lymphoma(NHL).Methods 45 patients failing to CHOP or CHOP-like regimen were treated with MINE regimen and every patient had received 2-6 cycles of treatment. IFO 15 g/m2, ivgtt on d1-3, combined with mesna 400 mg iv for3 times on Oh, 4h, 8h after IFO ivgtt; MIT 10 mg/m2 ivgtt on dl; VP1665 mg/m2 ivgtt on d1-3. 21-28 daysconsisted of one cycle. The therapeutic effects and side effects were evaluated according to the WHO criteria.Results In all 45 patients, 53.3 % of overall response rate were achieved CR for 9 cases, 15 cases PR, 13 cases SD and 8 cases PD. Main side effects were myelosuppression, nausea, vomit, alopecia, etc. Conclusion MINE regimen is an effective and safe salvage regimen in treating patients with recurrent and refractory intermediate grade NHL. Further clinical study on the application of MINE regimen is recommended.

The water extract frem the style of Zea mays L.showed a therapeutic effect on diabetes caused by alloxan, and obviously reduced blood glucose level of heperglycemia induced by glucose or epinephrine in mice, but no affect in normal mice, when it was administrated orally. In addition, it could decrease cholesterin content.

Objective To discuss the role of matrix metalloproteinase-8(MMP-8) mRNA in apical granuloma and periapical cyst by detecting the its expressions in the periapical granuloma and periapical cyst.Methods Reverse transcription polymerase chain reaction(RT-PCR) technique was used to measure the expressions of MMP-8 mRNA in 22 cases of periapical granuloma,11 cases of periapical cyst and 10 cases of normal periapical tissues.Results The positive rates and the levels of MMP-8 mRNA expression in apical granulomas and periapical cysts were significantly higher than those in normal tissues(P0.05).Conclusion The expression of MMP-8 may play an important role in the pathogenesy of apical granuloma and periapical cyst,and it may be involved in the process of bone destruction.

Objective To investigate the influence of matrix metalloproteinases-9 (MMP-9) on permeability of blood-spinal cord barrier (BSCB) after spinal cord injury (SCI). Methods 68 male C57BL/6 mice were randomly divided into sham group, 2 days group (S2), 7 days group (S7) and 14 days group (S14) after SCI with 17 mice in each group. All the groups received a moderate impacted spinal cord injury except the sham group. Evan's Blue (EB) was administered intraperitoneally to detect the permeability of BSCB. Occludin was analyzed by immunofluorescence, the expressions of occludin and MMP-9 were detected by Western blotting. Results After SCI, BMS score significantly reduced, compared with S2 group, S14 group showed a significant increase (P<0.01). The permeability of BSCB was seriously damaged after SCI. Compared with S2 group, S14 group showed a notable down-regulation in the permeability of injured micro-vessels (P<0.05). The expression of occludin was down-regulated and the expression of MMP-9 was up-regulated 7 days after SCI (P<0.05). Compared with S2 group, S14 group showed a significant up-regulation of occludin and a remarkable down-regulation of MMP-9 (P<0.05). Conclusion After SCI, MMP-9 might mediate the expression of occludin to influence the BSCB permeability.

Setting up the innovative and entrepreneurship education and teaching system is the important content of the current university education reform. Through the idea of leading innovation with entrepreneurial practice, we have implemented a series of education reforms in the students of medical laboratory technology, reformed the teaching methods and content, set up the tutors for undergraduates and improved the scientific research ability of students. The results show that the education reforms have enhanced the ability and sense of innovation, providing a good reference for the reform of innovation and entrepreneurship education in medical laboratory technology.

Objective To observe the effects of melatonin on synaptic plasticity impaired by spinal cord injury in rats. Methods A total of 54 female Sprague-Dawley rats were divided into sham group (n=18), control group (n=18) and melatonin group (n=18). Spinal cord inju-ry model was established with modified Allen's method at T10 (10 g from 25 mm height). The number of neurons and the expression of the Nissl body were detected with immunofluorescence and Nissl staining. The expression of neurofilament-200 (NF-200), brain-derived neuro-trophic factors (BDNF), Synapsin I and growth-associated protein-43 (GAP-43) was detected with Western blotting. Results Seven days af-ter injury, the number of motoneurons, the expression of Nissl body in motoneurons, and the expression of BDNF, Synapsin I and GAP-43 decreased in the control group compared with those in the sham group, and they increased in the melatonin group compared with those in the control group. Conclusion Melatonin can repair the impaired synaptic plasticity, which might promote the functional recovery after spi-nal cord injury.

ObjectiveTo examine the effects of temsirolimus, an inhibitor of mammalian target of rapamycin, on bladder cancer cell lines T24 and BIU-87 in vitro and in vivo for purpose of evaluating the probability of mTOR targeted therapy for bladder cancer.MethodsAfter being treated by a different concentration of temsirolimus, T24 and BIU-87 cells were tested by MTT assay for cell proliferation activity.Cell cycle and apoptosis analysis were performed with flow cytometer. Wound scratch assay was used for cell migration activity and transwell motility assay. Western blot analysis was used to test the mTOR phosphorylation. Subcutaneous inoculation of 6-week-old nude mice was performed using 1 × 106 T24 cells in 50％ matrigel for both control (n = 10) and temsirolimus (n = 10) groups. The volume of tumors was examined and then the expression of Ki-67 was detected by immunohistochemistry.ResultsTemsirolimus significantly inhibited proliferation of T24 and BIU-87 cells in a dose- and time-dependent manner. After administration of temsirolimus on T24 and BIU-87 cell lines for 24 h, the rate of wound healing in 0 nmol/L groups were (88.9 ± 14. 1 ) ％ and ( 83.6 ± 16.3)％ , which were higher than in the 5 nmol/L groups, which were (42.7 ± 11.6) ％ and ( 36.9 ± 9.7 ) ％ ( P ＜ 0.05 ). In the transwell motility assay, the number of cells in the 0 nmol/L group was 26.5 ± 5.8 and 28.2 ± 4.6, which was higher than in the 5 nmol/L group ( 19.0 ±3. 8 and 21.3 ± 5.1, respectively) (P ＜ 0. 05). When temsirolimus was administered on T24 and BIU-87 cell lines for 48 h the percentages of cells delayed in phase G0/G1 in 5 nmol/L group were ( 77.46 ±6.11)％ and (73. 39 ± 4. 94)％ respectively, and higher than in the 0 nmol/L group, which were (65.99 ±5.01 )％ 、(60.15 ±3.98)％ (P ＜0.05). There was no statistically significant difference in the apoptosis rate between the two groups (P ＞ 0.05 ). In Western blot analysis, the ratios of p-mTOR/β-actin were 0.92 ±0.09 and 1.01 ± 0.08 in 0 nmol/L group, and higher than in the 5 nmol/L group (0.47 ±0.05、0.04 ±0. 01 ) (P ＜ 0.05 ). After administration of temsirolimus for 21 days, the tumor volume in nude mice in the control group were 351.1 ± 139.9 mm3 , which was larger than 351.1 ± 139.9 mm3 in the temsirolimus group ( P ＜ 0.05 ). The positive rate of Ki-67 expression was ( 67.3 ± 8.4 ) ％ in the control group, which was higher than in the temsirolimus group ( 35.5 ± 6.7 ) ％ ( P ＜ 0.05 ).ConclusionsThis study provides in vitro and in vivo evidence that temsirolimus may inhibit the viability of bladder cancer cells and temsirolimus could be exploited as a potential therapeutic strategy in bladder cancer.