Objective To investigate the expression of NOD2 and TLR9 in gastric carcinoma and precancerous lesions and their clinical significance. Methods The expression of NOD2 and TLR9 was measured by immunohistochemical staining (S-P method) in 84 patients with atrophic gastritis, 48 eases with gastric ulcer, 80 cases with gastric adenocarcinoma and 40 eases with superficial gastritis. Results NOD2 and TLR9 expression was up-regnlated in superficial gastritis, atrophic gastritis, gastric ulcer and gastric adenocarcinoma. The positive expression rate of NOD2 was 35% ,21% ,33% ,40% respectively, and the positive expression rate of TLR9 were 15%, 12% ,21% ,22% respectively. The expression of NOD2 and TLR9 in Hp complicated atrophic gastritis, gastric carcinoma was significantly higher than that of lip not complicated disease entities ( P < 0. 05 ). Conclusions The expression of NOD2 and TLR9 may be valuable index for predicting the development of gastric mucosal damage from superficial to atrophic gastritis, gastric ulcer and gastric carcinoma.

Objective To examine whether IFN-λ has the ability to inhibit HIV-1 infection of blood monocyte-derived macrophages and its mechanism(s). Methods Macrophages were pretreated with IFN-λ/ IFN-λ2 for 24 h before infected by HIV-1 R5 strains (Bal, Jago, and JRFL). And then the culture supernatants were detected HIV-1 reverse transcription (RT) activity and p24 protein expression by HIV-1 BT assay and ELISA. The expressions of IFN-λ receptor, CD4, CCRS, CXCR4 were evaluated by real-time PCR. Results Both IFN-λ1 and IFN-λ2, when added to macrophage cultures, inhibited HIV-1 infection and replication. This IFN-λ-mediated anti-HIV-I activity is broad, as IFN-λ could inhibit infection by both laboratory-adapted and clinical strains of HIV-1. Investigations of mechanism(s) responsible for the IFN-λ action showed that although IFN-λ had little effect on HIV-1 entry receptor CD4 and co-receptor CCR5 and CXCR4 expression, IFN-λ inhibited HIV-I infection of macrophages through connecting with IFN-λ recep-tor. Conclusion IFN-λ could inhibit HIV-I replication in macrophages. These findings indicate that IFN-λ may have a therapeutic value in the treatment of HIV-1 infection.

Objective To discuss operative treatment of old fractures of pelvis. Methods Since February 2000, 14 cases with old fracture of pelvis have been treated in our hospital with operative scar or callus settlement, traction and reduction, and internal fixation. Results All patients were followed up from 6 months to 3 years and 6 months. According to such indexes as pelvic shape, length difference between lower limbs, gait, and radiogram, the scores were excellent in 8 cases, fine in 4 and poor in 2, with the excellent and good rate being 85.7%. Conclusion With reasonable design and appropriate method, operative treatment is fit for old fractures of pelvis because it can avoid severe disability and improve the quality of life of the patients.