The direct spinal projections from the cerebellar nuclei in the rabbit were retro gradely traced by unilateral injection of WGA-HRP into different levels of the spinal cord, including the cervical, thoracic and lumbar segments. The labeled neurons in the cerebellar nuclei were constantly seen in those animals, in which the upper cervical segments (C_(2-4)) were injected. No labeled neurons could be found in the cerebellar nuclei following injection into the lower cervical cord (C_(6-8)) or more caudal segments. All labeled neurons were located in the caudal parts of the fastigial and the interposed nuclei on the side contralateral to the injection. The results show that there are crossed projections from the cerebellar nuclei directly to the upper cervical cord. This study provide certain morphological evidences for further investigation of some aspects of cerebellar functions on motor coordination.

Following injection of HRP into the cerebellum of the rat, the retrogradely labeled′spinal border cells′ in L_1-L_3 of the spinal cord were examined electron microscopically for understanding the synaptology. Six types of terminal boutons (S, F, Cf, T, G and P types) which terminated on the surface of cell bodies or dendrites of the spinal border cells were recognized. The S- and F-boutons contain spherical and elliptical vesicles respectively. These two types of boutons had relatively broad area contacting with the surface of either cell bodies or dendrites. They could be derided into two sub-types respectively. One was the elonagted giant bouton and the other was the round. Cf-type contain flattened vesicles and it make membraneous′contact′ lacking in specialized pre-and postsynaptic membrane thickenings. T-type contain spherical and large granular vesicles, and dense body was seen beneath the post synaptic membrane. G-type boutons contain large granular vesicles in area of presynaptic. P-type boutons form synapses upon the large S-type boutons and contain pleomorphic vesicles. The postsynaptic membrane of S-, T- and G-types is apparently thicker than the presynaptic membrane and showed to be asymmetrical. Further study is necessary with regard to the sources of different terminal boutons contacting with different portions of the spinal border cells.

ABSTRACTOBJECTIVE To observe the preventive effect of oxaliplatin induced peripheral neuropathy treated by fumigation of Siteng Yixian decoction.METHODS Totally 150 colorectal cancer patients from oncology department Of Hainan provincial hospital of TCM between January 2010 and December 2014 were enrolled in this study.And they were randomized into two groupswith 75 cases in each.The patients in the control group only received oxaliplatin based chemotherapy.While the pa-tients in the treatment group were given the chemotherapy combined with fumigation of Siteng Yixian decoction.The fumiga-tion lasted for forty minutes a timetwice a day.After the treatment of two cycles chemotherapythe incidence rate and sever-ity classification of peripheral neuropathy was analyzed one month later to evaluate the preventive effect.RESULTS The difference of incidence rate of peripheral neuropathy was significantwith the rate being 18.7% in the treatment group and 56. 0% in the control groupP <0.01.The severity classification was evidently higher in the control group90.37than those in the treatment group60.63and there exised a statistical significanceP <0.05.Among the patients who had second degree peripheral neuropathy or abovethe oxaliplatin accumulative dose was1 054.76±124.6in the treatment group and823.47 ±190.67 in the control groupand the difference was statistically significantP <0.05.Logistic regression indicated that fu-migation of Siteng Yixian decoction was the protective factor for the oxaliplatin?induced peripheral neuropathy.CONCLUSION Fumigation of Siteng Yixian decoction can reduce the incidence ratedecline the severity classification of oxaliplatin?induced peripheral neuropathy and increase the oxaliplatin tolerance dose.It is the independent preventive factor for oxaliplatin?induced peripheral neuropathy.