1.Treatment of Knee Osteoarthritis with Small Needle Knife Coordinates Joints Washing
International Journal of Traditional Chinese Medicine 2009;31(2):148-149
Objective To observe the effect of treating knee osteoarthritis with small needle knife plus joints washing. Methods All cases were randomly recruited into a control group(73 cases) and a treatment group(75 cases). The treatment group was treated with small needle knife plus joints washing, and the control group was treated with meloxicam and applying diclofenae extemally. Methods The curative rate showed significant difference between the two groups(X2= 19.62, P<0.01 ), while the total effective rate showed on obvious difference(X2=2.12, P>0.05 ).Results Joint function of patients in the treatment group was notable improved after the treatment (X2=9.21, P<0.01) . Conclusion The therapy with small needle knife plus joints washing has good effect in treating knee osteoarthritis.
2.Changes of Blood Lipid,Blood Glucose and Blood Uric Acid Levels in Patients with Acute Cerebral Infarction and Its Clinical Significance
Journal of Guangzhou University of Traditional Chinese Medicine 2000;0(04):-
0.05),but the difference of serum low density lipoprotein cholesterol(LDL-C),glucose(Glu)and uric acid(UA)levels was significant(P
3.Determination of Entrapment Efficiency of Gemcitabine Hydrochloride Liposome
China Pharmacy 2005;0(19):-
OBJECTIVE: To establish a method for determining the entrapment efficiency of gemcitabine hydrochloride liposomes(GHL).METHODS: The liposome and the free gemcitabine hydrochloride were separated by ultrafiltration,and the content of free gemcitabine hydrochloride was determined by HPLC.The entrapment efficiency of gemcitabine hydrochloride liposomes was computed as well.RESULTS: The recovery rate in ultrafiltration methods was 97.8%~100.1% for blank substance versus 99.0%~100.1% for sample.The linear range of gemcitabine hydrochloride was 1.0~80.0 mg?L-1(r=0.999 3) and its average recovery rate was 98.7~101.2%,and its inter-day and intra-day RSD were all less than 3% and its average entrapment efficiency was 81.21%.CONCLUSION: The method is simple,accurate and applicable for the determination of the entrapment efficiency of gemcitabine hydrochloride liposomes.
4.Treatment of ureteral calculus with transurethral ureteroscopic pneumatic lithotripsy
Jianchang LI ; Huiqing ZHANG ; Yingjie ZHANG
Chinese Journal of Minimally Invasive Surgery 2001;0(03):-
Objective To investigate therapeutic effects of transurethral ureteroscopic pneumatic lithotripsy(TUPL).Methods A retrospective analysis was made on 320 cases of ureteral calculus treated with TUPL from September 2002 to August 2005.A Wolf F9.8 rigid ureteroscope and an EMS pneumatic ballistic lithotriptor were used.The probe was 0.8 mm or 1.0 mm in diameter.The pulse settings was 200~300 kPa.The fragmentation was conducted with single or multiple episode of treatment.Results The stone was pushed back to the renal pelvis in 6 cases and a conversion to extracorporeal shock wave lithotripsy(ESWL) was needed.The insertion of the ureteroscope was failed in 5 cases and a conversion to open surgery was required.The TUPL time was 2~10 min(mean,5.5 min) and the intraoperative blood loss was 3~15 ml(mean,7 ml).The stones were successfully fragmented on one session in 309 cases,the success rate being 96.6%(309/320).The length of postoperative hospital stay was 3~7 d(mean,4.5 d).There were 24 cases of mucosal injury of the ureter and 4 cases of false passage,all of which were cured with double-J catheter indwelling for 1 month.Follow-up examinations for 1~10 months(mean,4 months) showed stone clearance within 1 month and no ureteral stenosis or urinary infection.Conclusions Transurethral ureteroscopic pneumatic lithotripsy for the treatment of ureteral calculus is safe and effective.
5.Dengue virus specific T cells are involved in the pathogenesis of dengue virus infection
Yingjie WAN ; Junlei ZHANG ; Jiali WANG ; Jing AN
Journal of Third Military Medical University 2003;0(21):-
Objective To study the roles of dengue (DEN) virus specific T cells in the pathogenesis of DEN virus infection. Methods 2D42 cells, DEN virus specific CD8 + cell clones, were employed to investigate their in vivo function in DEN virus infection using an animal model. HepG2 was implanted into mice with severe combined immunodeficiency disease (HepG2-SCID) for the establishment of HepG2-SCID model. The animals were divided into 3 groups: Group A: HepG2-SCID mice were inoculated with 2D42 cells and then infected with DEN virus type 2 (DEN2) intraperitoneally; Group B: HepG2-SCID mice were inoculated with normal mouse thymuscytes (NMT) and then intraperitoneally infected with DEN2; Group C: HepG2-SCID mice were intraperitoneally infected with DEN2 alone. The mortality, viremia, and frequency of histopathological changes in the major organs of mice in the three groups were observed after infection. Results After inoculation of 2D42 cells, 80% infected mice showed severe clinical signs and died at the average 12.8 d after infection. The others only had transient manifestations, and then recovered from the disease and survived for more than 3 months. In contrast, after inoculation of NMT and /or DEN2 alone, 100% mortality rate was noted in these two groups. High viremia and frequency of histopathological changes in the major organs were observed in the mice in groups A and B. Conclusion Our data support both protective and pathogenic roles for DEN-specific CD8 + T cells in DEN virus infection.
6.Propofol proteets liver in hypoxia/reoxygenation injury in rats
Yingjie SUN ; Guangjie GAO ; Tiezheng ZHANG
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To investigate the protective effects of propofol on liver against hypoxia/reoxgenation (H/R) injury in rats. Methods Twenty-four Wistar rats of both sexet weighing 200-250g were randomized into control group (group A), hypoxia/reoxygenation group (group B) and H/R + propofol group (group C). The 8-iso-prostaglandin F 2? (8-iso-PGF 2? ) level in the hepatic tissue was determined by enzyme-linked immunosorbent assay (ELISA). The content of NO and the activity of NOS were assessed by biochemical methods. Results In group B, the 8-iso-PGF 2? level in hepatic tissue was significantly higher than that in group A (P
7.Preparation of harmine hydrochloride liposome by active loading method
Song DU ; Yingjie DENG ; Wei ZHANG
Chinese Traditional and Herbal Drugs 1994;0(05):-
Objective To prepare a harmine hydrochloride liposome formulation with high encapsulation efficiency. Methods Preformulation investigation was carried out to obtain the drug physicochemical properties such as solubility and lgD in buffers of different pH value. Harmine hydrochloride was encapsulated into liposomes by active loading method. Encapsulation efficiency of liposomes was determined after the free drug was separated from liposome by ultrafiltration. The influence factors on the encapsulation efficiency including drug-lipid weight ratio, incubation temperature, pH value of external water phase were investigated. Results As the pH value increasing, the solubility of harmine hydrochloride was decreased, while the apparent oil-water distribution coefficient was increased. By active loading method, the encapsulation efficiency could be over 80% when the drug to lipid weight ratio was under 1∶5. The pH gradient between intervesicle and intravesicle obviously influenced the encapsulation efficiency, while incubation temperature had little effect on encapsulation efficiency. Conclusion Active loading is suitable for preparing harmine hydrochloride liposome with high encapsulation efficiency.
8.The effects of midazolam and propofol on liver against hypoxia/reoxygenation injury in rats
Yingjie SUN ; Guangjie GAO ; Tiezheng ZHANG
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To investigate the preventive effects of midazolam and propofol on liver against hypoxia/reoxgenation(H/R) injury in rats.Methods Twenty-four Wistar rats of both sexes weighing 200-250g were randomized into four groups:control group(group A),hypoxia/reoxygenation(group B),H/R + midazolam(group C) and H/R + propofol(group D).The 8-iso-prostaglandin F2?(8-iso-PGF2?) levels in the hepatic tissue were determined by enzyme-linked immunosorbent assay(ELISA).The contents of nitric oxide(NO) and the activity of nitric-oxide synthase(NOS) in hepatic tissue were determined by biochemistry methods.Results After hypoxia and reoxgenation injury in rats,the 8-iso-PGF2? level of hepatic tissue in group B was significantly higher than that in group A(P
9.Using FRET Technique to Investigate The Apoptotic Mechanism Induced by Cisplatin in Living Cells
Lei LIU ; Yingjie ZHANG ; Xianwang WANG
Progress in Biochemistry and Biophysics 2006;0(09):-
Cisplatin, an efficient anticancer agent, can trigger multiple apoptotic pathways in cancer cell. However, the signal transduction pathways in response to cisplatin-based chemotherapy are complicated, and the mechanism is not fully understood. Using fluorescence resonance energy transfer (FRET) technique, the molecular mechanism of cisplatin-induced apoptosis in living human lung adenocarcinoma cells (ASTC-a-1) were investigated. After cisplatin treatment, the recombinant pFRET-Bid and pSCAT-3 probes were used to determine the kinetics of Bid cleavage and Caspase-3 activation, respectively. The fluorescence probes Bid-CFP and DsRed-Mit were also used to detect the spatial and temporal changes of Bid in real-time in sub-cell level. The results showed that a cleavage of the Bid-FRET probe occurring at about 4~5 h after treated with 20 ?mol/L cisplatin. Cleavage of the Bid-FRET probe coincided with a translocation of tBid from the cytosolic to the mitochondria, and the translocation lasted about 1.5 h. At the anaphase of cell apoptosis, Caspase-3 was activated obviously as detected by FRET and Western blotting techniques. Using real-time single-cell analysis, it was observed the kinetics of Bid and Caspase-3 activation for the first time in living cells during cisplatin-induced apoptosis.
10.Biocompatibility and clinical application of coronary stents
Yaguang HU ; Huijiu ZHANG ; Yingjie YANG
Chinese Journal of Tissue Engineering Research 2007;0(17):-
Resulting from the shape stability, metal vascular stent has limited the vascular retraction, and subsequently prevent the unfavorable vascular remodeling. However, the metal stent requires further anticoagulant therapy after implantation, induces the hyperplasy of vascular smooth muscle cells, and cannot completely prevent the occurrence of in-stent restenosis. Surface modification of metal stent may reduce thrombogenesis. Based on the metal stent, drug eluting stent can transfer the active drugs to the damaged vessels, release them into the vascular wall and inhibit the in-stent restenosis. The eluting drugs restrain both the proliferation of smooth muscle cells and the regeneration of normal endothelial cells, leading to delay the vascular endothelialization and increase the risk of delayed thrombogenesis. The effect of stent implantation on the modus and size of vascular injury varies according to different operational techniques, processing technologies and designs, thus influencing the occurrence of in-stent restenosis. It is a potential study topic of interventional therapy to develop new eluting materials and eluting drugs, modify formulation, as well as facilitate the stent structure.