Objective To study the abnormalities in defecation of patient after laparoscopic cholecystectomy as well as the influencing factors. Method A self-designed questionnaire and the anxiety self-rating anxiety scale (SAS) were used to investigate the defecation conditions of patients after laparoscopic cholecystectomy. Results About 40.78% of the patients were normal in defecation after the surgery. Among those with abnormalities in postoperative defecation, 30.16%had diarrhea. The influenced factors included age, preoperative gallbladder function, daily diets and anxiety. Conclusions The abnormalities in defecation of the patients after laparoscopic cholecystectomy are common. The patients with young age, the gallbladder function before laparoscopic cholecystectomy and anxiety diet without limit need more attention.

10 and Foxp3 expression, reduce the IL-17 and ROR-γt expression and adjust Treg/Th17 immune balance; effect of 0.1 mg/mL FSN +0.1 mg/mL MTX is the most obvious.

0 05) The parameters did not change significantly with increasing MAC in group D, whereas LESP, BrP and PVV decreased significantly in a dose dependent manner in group E (P

Objective To investigate the protective effects of propofol on liver against hypoxia/reoxgenation (H/R) injury in rats. Methods Twenty-four Wistar rats of both sexet weighing 200-250g were randomized into control group (group A), hypoxia/reoxygenation group (group B) and H/R + propofol group (group C). The 8-iso-prostaglandin F 2? (8-iso-PGF 2? ) level in the hepatic tissue was determined by enzyme-linked immunosorbent assay (ELISA). The content of NO and the activity of NOS were assessed by biochemical methods. Results In group B, the 8-iso-PGF 2? level in hepatic tissue was significantly higher than that in group A (P

Objective To investigate the preventive effects of midazolam and propofol on liver against hypoxia/reoxgenation(H/R) injury in rats.Methods Twenty-four Wistar rats of both sexes weighing 200-250g were randomized into four groups:control group(group A),hypoxia/reoxygenation(group B),H/R + midazolam(group C) and H/R + propofol(group D).The 8-iso-prostaglandin F2?(8-iso-PGF2?) levels in the hepatic tissue were determined by enzyme-linked immunosorbent assay(ELISA).The contents of nitric oxide(NO) and the activity of nitric-oxide synthase(NOS) in hepatic tissue were determined by biochemistry methods.Results After hypoxia and reoxgenation injury in rats,the 8-iso-PGF2? level of hepatic tissue in group B was significantly higher than that in group A(P

Codon is an important carrier of genetic information in organisms. In evolution, codon bias is formed in many organisms. In this study, the analysis of expressed genes in Lonicera japonica Thunb. is presented. The codon bias of genes with complete coding region is analyzed through CodonW. And we calculated the codon usage bias of hydroxycinnamoyl-CoA: quinate hydroxycinnamoyl transferase (HQT) genes. The results showed that L. japonica were bias toward the synonymous codons with A and T at the third codon position.

Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1～1 800 nt and 1～600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18～28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.

Objective To assess the relationship between the concentration of total suspended particle (TSP) and cerebral-cardiovascular disease mortality of urban residents in Fushun city, China. Methods The data of cerebral-cardiovascular disease mortality and TSP concentration from 1999 to 2003 in Fushun city were collected. The association between TSP concentrations and the mortality of cerebral-cardiovascular diseases was analyzed using Poisson regression model adjusted for seasons, long-term patterns and meteorological variations using an ecological parametric method. Results As the concentration of TSP increased by 50 ?g/m3, OR of cerebral-cardiovascular disease mortality increase was 1.015 42 (95%CI=1.000 18-1.030 89) in the male group and 1.022 40 (95%CI=1.004 87-1.040 23) in the aged male group respectively, as 4-days lag TSP concentration increased by 50 ?g/m3, OR of cerebral-cardiovascular disease mortality increase was 1.008 26 (95%CI=1.000 57-1.016 02) in the whole people, 1.016 27 (95%CI=1.006 71-1.025 93) in the male group and 1.016 65 (95%CI=1.005 25-1.028 19) in the aged male group respectively. Conclusion Air pollution by TSP is considered as a risk factor for the increase of cerebral-cardiovascular disease mortality in Fushun city, China.

Objective To evaluate the effects of different sedation depths of propofol on brain injury in patients undergoing cardiac valve replacement with cardiopulmonary bypass (CPB).Methods Forty-five ASA physical status Ⅲ or Ⅳ patients of both sexes,aged 26-64 yr,weighing 50-85 kg,scheduled for elective cardiac valve replacement with CPB,were divided into 3 groups according to intraoperative sedation depths (n =15each):30 ≤ bispectral index (BIS) value ＜ 40 (group A) ; 40 ≤ BIS value ≤ 50 (group B) ; 50 ＜ BIS value ≤ 60(group C).Anesthesia was induced with iv injection of lidocaine,midazolam,sulfentanil,etomidate and vecuronium.The patients were tracheally intubated and mechanically ventilated.PET CO2 was maintained at 35-45 mm Hg.Anesthesia was maintained with iv infusion of propofol at a rate of 7,5 and 3 mg· kg-1 · h-1 in A,B and C groups,respectively,intermittent iv boluses of sulfentanil and pipecuronium,and intermittent inhalation of sevoflurane.The infusion rate of propofol was adjusted to maintain the corresponding anesthesic depth in each group.Before CPB (T1),at 30 min of CPB (T2),immediately after termination of CPB (T3) and at 1 h after the end of CPB (T4),blood samples were obtained from the jugular bulb vein and radial artery for determination of plasma S-100β protein concentration and for blood gas analysis,and the arterial to venous blood oxygen content difference (Dj-aO2),cerebral O2 extraction rate (CERO2) and jugular bulb venous to arterial blood lactic acid content difference (Dj-aL) were calculated.Results Compared with group C,the plasma S-100β protein concentrations were significantly decreased,SjvO2 was increased,and Dj-aO2 and CERO2 were decreased at T3-4,and Da-jL was decreased at T3 in groups A and B (P ＜ 0.05).Compared with group B,the plasma S-100β protein concentrations were significantly decreased at T3-4,SjvO2 was increased at T2,Da-jO2 and CERO2 were decreased at T2,and DajL was decreased at T3 in group A (P ＜ 0.05).Conclusion Moderately deepened anesthetic depth of propofol (30≤ BIS value ＜ 40) is helpful in attenuating the brain injury in patients undergoing cardiac valve replacement with CPB.

Bone-modifying agents (BMA) is a series drugs to alleviate the pain,pathological fractures,spinal cord compression,hypercalcemia,bone-related events which induced by bone metastases.Bisphosphonate drugs and denosumab are two dominant kinds of BMA at present.It has been proved that BMA is used in bone metastases patients with bone destruction,as adjuvant therapy for chemotherapy and radiotherapy,which can significantly improve the efficacy and prolong the survival of patients.In addition,some traditional Chinese medcine can effectively relieve a series of related symptoms caused by bone metastases and improve prognosis.Choosing right medication in clinical work can maximize the reduction of pain caused by bonerelated events and improve the quality of life of patients.