Aim To establish the metho d of precise lung slicing and examine the validity of neutral red(NR) assay for measuring lung slices viability.Method Inflated with 1% low melting agaro se solution, lung lobes were cut into slices by shaking microtome. With the thic kness of 400,500,600,700 ?m,lung slices were cultured in medium pH 6.8,7 .0,7.2, 7.4, respectively. After 1 h pre-incubation, lung slices were cont inuously submerged in 24-well plate, incubated for 0,2,4,6 h,respectively. NR assay, MTT assay, LDH leakage and SOD activity were used to assess the slices viability under different slices thickness, medium pH and culturing time. Result When the slice thickness was 600 ?m and medium pH was 7.0, t he viability of slices maintained best and steady for 6 h. There were positive c orrelations between NR uptake and MTT reduction in slices under different thickn ess(r 1 = 0.91, P

Objective To explore the problems existing in management of medical records under new situation,especially the cognition of medical workers when the patients required to seal up or seize medical records.As a result,we further discussed existing problems in management of medical records under new situation and the countermeasures. Methods Self-designed questionnaires were used to investigate 75 doctors and 75 nurses.The results underwent analysis. Results The analysis showed that 27.7% doctors and 29.4% nurses didn't know any question.While 50.0% doctors and 55.7% nurses didn't know how to deal with when patients wanted to seal up the medical records,which became the issue that most doctors and nurses did not know. Conclusions Doctors and nurses should be familiar with concerned legal knowledges and strengthen the consciousness of medical records management in order to make the medical records the real evidence in the dispute settlement or court of law.

The injurious changes of the microvasculature in the maxillofacial region due to high-velocity bullet injury were observed in different time intervals with scanning and transmission electron microscopy. It was found that there were microthrombus formation in the vascular lumen, and endothelial loss and dege-neration or necrosis of the vascular wall in the microvasulature around the wound. Our findings indicate that when early reconstructive surgery for the tissue defect due to gunshot wound especially for those in the maxillofacial region is to be performed with microsurgical technique, it is essential for the surgeon to realize and consider the wounding condition on the microvasculature of the recipient site.

Intracranial artery stenosis is one of the main causes inducing transient ischemic attack (TIA) or cerebral ischemic stroke. Being a minimally-invasive and reliably-effective technique, intracranial artery stent angioplasty has brought about a new approach for the treatment of intracranial artery stenosis , and thus provides more opportunities to the patients who are not suitable for intracranial vascular bypass surgery. However, the higher occurrence of perioperative complications caused by stent angioplasty should be seriously taken into consideration by clinical physicians. In order to reduce the occurrence of complications , in this paper the perioperative complications of stent angioplasty for intracranial artery stenosis are analyzed , and the therapeutic strategies as well as the proper approaches are discussed.

Objective To study the exposure dose-effectiveness of ? irradiation on killing the plasmodium in the mice RBC,for the further exploration on the method that could kill the plasmodium in RBC without affecting the activity and function of normal RBC. Methods After infection with Plamodium yoelii (P.y),blood was collected from mice and exposed to ? irradiation (radiated group). An unirradiated group served as control. In the irradiated group,P.y infected blood was divided into three aliquots,each aliquot was irradiated one time by ? radiation using Gammacell 1000 Elite blood radiation apparatus. The dosage of each aliquot was 25,35 and 45Gy. After irradiation,the blood samples were stored at 4℃. Then mice were inoculated with these irradiated blood stored for 1,3 or 5 days after irradiation,or with unirradiated blood. Two days later,the blood samples were taken from inoculated mice and were examined under microscope and plasmodium infection rates were calculated. Results The mice in the control group had parasitemia much earlier than those in irradiated group (1—2 days),and the plasmodium infection rate in the control group was significantly higher than that in the irradiated group(3.7% vs 0.07%). With increasing dosage of irradiation,the survived plasmodium in blood decreased,and survival of mice increased(8—12 days). After 45 Gy irradiation and 5 day storage at 4℃,there were no plasmodium found in the red blood cell of inoculated mice. In the control group,blood testing result was positive,and all the mice died.Conclusion Plasmodium in mice RBC can be killed effectively when blood is exposed to 45Gy irradiation and stored at 4℃ for 5 days.

Child, Preschool ; Continuous Positive Airway Pressure ; Humans ; Infant ; Laryngocele ; surgery ; Postoperative Period ; Ventilator Weaning

In the progress of viral hepatitis, lymphocytes migrate and infiltrate liver tissues, which is relevant to antiviral therapy.However, the related mechanism is still poorly understood.In recent years,CXC chemokine ligand (CXCL)10 and its receptor CXCR3 have been extensively studied.The interaction between CXCL10 and CXCR3 would be a breakthrough point for study of the mechanism of viral hepatitis pathogenesis.This article reviews the research advances of CXCL10 in the progression of viral hepatitis and in the evaluation of antiviral therapy.

Objective To study the expressions of Chemerin in hepatocellular carcinoma (HCC) and HCC cell lines,and to demonstrate the relationship between the expressions and prognosis.Methods The expressions of Chemerin protein in normal hepatocellular tissues,HCC and their paired tissues were detected by immunohistochemical staining of SABC; the expressions of Chemerin protein in HCC,cell lines and immortalized hepatocyte cell lines were detected by RT-PCR.Results The positive expression rates of Chemerin were 56.67％,90.00％,100％ in HCC,HCC cell line and normal hepatocellular tissues respectively,and the differences were significant (P＜0.05).The expressions of Chemerin mRNA in HCC paired tissues were higher than in HCC (P＜0.05).There was a higher expression of Chemerin mRNA in immortalized hepatocyte cell line LO2 ; but a lower expression in HCC cell lines.The expressions of Chemerin were related to lymph node metastasis,portal vein tumour thrombi,differentiation and TNM stage but not related to sex,age,tumour size,HBsAg and AFP.Conclusions Down-regulated expression of Chemerin may play an important role in the development,progression and metastasis of HCC.It may be a molecular marker for prognosis of HCC.

BACKGROUND: Fracture is the main clinical manifestation of osteoporosis. Thus, a safe, feasible and low cost bone mineral density(BMD) measurement can help to prevent the osteoporosis-related fracture and other osteoporosis-related complications in clinical practices.OBJECTIVE: Quantitative ultrasound(QUS) measurement systems have been employed to evaluate bone status in middle-aged and aged females for better diagnoses and the prevention of osteoporosis-related fracture.DESIGN: Case controlled trial based on patients and healthy individuals.SETTING: The study was conducted at the orthopedic department of a municipal hospital.PARTICIPANTS: The study was conducted in the Orthopedic Department of the Sixth People' s Hospital of Guangzhou from February to September in 2002. Seventy-four middle-aged and aged female patients, diagnosed with fracture, entered the study procedure. A control group of 218 age-matched postmenopausal women was recruited for comparison. The patients in the control group were divided into 5 subgroups: 41 - 50 years old, 51 -60 years old; 61 - 70 years old; ＞ 80 years old. Both the patients and the healthy controls were divided into several groups according to their ages. Then each of them was measured by QUS, and the results were analyzed and compared among all the groups.MAIN OUTCOME MEASURES: The broadband ultrasound attenuation (BUA) and the speed of sound(SOS) of all the patients and the healthy women in this study.RESULTS: BUA values of over 61 years old patient groups equaled to (58.2 ± 6. 8), (54.2 ± 4.8) and(47.8 ± 3.5) dB/MHz respectively. The values were significantly different from those of the control group( P ＜ 0. 01 ).The SOS values of the over-51-year-old patient groups were( 1489.6 ± 24. 3),(1486.6 ± 17.7), (1483.7 ± 18.4) and(1442.7 ± 18.3) m/s respectively. They were also significantly different from those of the control group( P＜ 0.01).CONCLUSION: QUS measurement system not only can be used to obtain quantitative data of the bones but also can be used to evaluate the bone structure. It has various advantages such as no radiation, low cost and easiness of operation. It is a valuable method that can greatly contribute to early prediction, early diagnosis and timely treatment of osteoporosis-related fracture.

Objective To study the impact of vaginal mesh exposure on quality of life in patients undergoing transvaginal reconstructive pelvic surgery (RPS) with polypropylene mesh.Methods From May 2004 to March 2011,114 patients with severe pelvic organ prolapse(POP) undergoing transvaginal RPS with polypropylene mesh were enrolled in this study,which were divided into exposure and non-exposure group according to appearing vaginal mesh exposure at 2 months,6 months and 1 year after operation.At the same time,pelvic floor distress inventory short form 20 ( PFD1-20 ) and pelvic floor impact questionnaire short form 7 ( PFIQ-7 ) were completed in those patients.Results At 2 months after operation,96 patients were followed up,including 19 patients in exposure group and 77 patients in non-exposure group,and the rate of exposure was 19.8c (19/96); At 6 months after operation,85 patients were followed up,including 13 patients in exposure group and 72 patients in non-exposure group,and the rate of exposure was 15.3％( 13/85 ) ; At 1 vear after operation,77 patients were followed up,including 6 patients in exposure group and 71 patients in non-exposure group,and the rate of exposure was 7.8％ (6/77).Mean score of PFDI-20 and PFIQ-7 in exposure group before operation was 39.6 and 57.1,which was statistically improved to 8.3 and 9.5 at 2 months after operation,8.3 and 9.5 at 6 months after operation,2.1 and 0 in I year after operation (P ＜0.01 ). Mean score of PFDI-20 and PFIQ-7 of non-exposure group before operation was 54.2 and 66.7,which was improved to 8.3 and 4.8 at 2 months after operation,0 at 6 months and 1 vear after operation,but there was no significant difference in mean score of PFDI-20 and PFIQ-7 between the two groups (P ＞ 0.05 ).Conclusion Vaginal mesh exposure was common after transvaginal RPS with polypropylene mesh,however,most of them were moderate,and there was no significant impact on patients'qualifies of life.