Objective To investigate the application of EBV-DNA determineation by PCR and to analyze the clinical significance in 100 EBV positive patients.Methods EBV-DNA was detected in peripheral blood by PCR and fluorescence assay.The clinical features of 100 children with EBV-DNA positive were reviewed retrospectively.Results Patients had median ages of 3 years(range,1 month to 12 years).There were 65 patients under the age of 3,25 patients between 3 and 7,and the remained older than 7 years.Among these cases,the positive rate of EBV-DNA in IM group was 84.0%(84/100)and 5.0%(2/40)in the control group,the percent of positive in the former was significant higher than the later(P＜0.01).EBV-DNA concentration of patient with IM in early stage(75.52±175.11)×103copies/ml were higher than convalescence(0.67±2.27)×103copies/ml(P＜0.01).Conclusion The EBV-DNA detection by PCR is a quick,accurate and sensitive assay in diagnosis of EBV infection diseases.

BACKGROUND: Poly methyl methyacrylate (PMMA) has been widely used as a denture base material in dental field for a long time. However, the fracture of acrylic resin dentures is an unresolved problem in prosthodontics. Therefore, how to improve the flexural strength of repaired denture seems to be extremely important. OBJECTIVE: To study the effect of embedding different amounts of metal wires on the flexural strength of repaired denture bases. METHODS: Twenty-five rectangular specimens (50 mm× 30 mm × 2.5 mm) were fabricated using heat-cured acrylic resins and randomly and evenly divided into five groups: A, B, C, D, and E. All specimens were fractured through the use of universal testing machine, and the flexural strengths were tested. Following preparation of fracture surfaces, one to four metal wires were separately embedded in the groups B, C, D, and E. No metal wires were embedded in the group A. All fractured specimens were repaired using self-curing resins. The flexural strengths were measured again using the same testing machine, and the percentages of strength recovery were calculated. RESULTS AND CONCLUSION: After repair, the flexural strengths were obviously reduced (P < 0.01). Compared to group A, the percentages of strength recovery were significantly increased in groups C, D, and E (P < 0.05). There was no significant difference in the percentage of strength recovery among groups C, D, and E (P > 0.05). These results indicate that embedding two to four metal wires can improve the flexural strength of denture bases.

Objective To discuss the influence of nursing intervention on psychological state and postoperative conffort of diabetic patients with interventional therapy. Methods 49 patients who were to receive interventional therapy were randomized into the experimental group and the control group.The con-trol group received routine nursing while the experimental group was given systemic comfort nursing inter-vention based on routine nursing intervention.The anxiety status and incidence of complications before and after the operation were compared by χ2 and t test. Results The anxiety alleviation in the experimental group was better than that of the control group.The incidence of waist- back pain, dysuria, catheter emic-tion, anaesthesia of lower limbs, fidget in the experimental group also more significantly decreased com-pared with those of the control group. Conclusions Systemic nursing intervention can improve the pay-chological and postoperative comfort for diabetic patients with interventional therapy.

Objective:To elucidate the effects of calcitonin gene-re la ted peptide(CGRP) on the gene expression of osteoprotegerin(OPG) and receptor ac tivator of nuclear factor-?B ligand(RANKL) in rabbit osteoblasts. Meth ods:Osteoblasts were cultured in media containing 10 -10～10 -7 mol/L of CGRP. After 24-hour incubation,semi-quanitative RT-PCR was perfor med to detect the expression of OPG and RANKL mRNA,and with ?-actin mRNA as th e internal control. Results:CGRP increased the mRNA expressio n of OPG with the maximal effect at the concentration of 10 -8～10 -7 mol/L. CGPR downgulated the mRNA expression of RANKL dose-dependantly.C onclusion:CGRP may regulate the activities of osteoclasts by regulating gene expression of OPG/RANKL.

Objective To study the therapeutic value of a second ERCP for the patients with failure of pre-cut ERCP.Methods A total of 167 cases of pre-cut ERCP failure were recruited to the study,among which 109 cases were diagnosed as common bile duct stones and/or benign papillary stenosis,and 58 cases as biliopancreatic lesion.ERCP failed with standard intubation for more than 20 minutes,even with pre-cut or fenestration.A second ERCP was preformed after rest of 3-5 days.The position sequence of intubation for most patients was horizontal,the front and rear.Results The success rate was 79.6％ (133 cases) for the patients with a second ERCP,85 patients received the procedures via the horizontal intubation,36 via anterior,and 12 via posterior intubation.The treatments were performed after successful completion of the endoscopic cannulation.One patient had retroperitoneal infection with duodenal perforation,another patient had severe pancreatitis,who were cured by the intervention methods.Conclusion The success rate of a second ERCP is high with proficient intubation skills.

To evaluate multi-slice computer tomography (MSCT) in mediastinal lymph node metastasis of T1 and T2 non-small cell lung cancer (NSCLC). Methods:A total of 32 patients with T1 and T2 NSCLC from February 2004 to October 2012 were selected. Preoperative MSCT assessment of mediastinal lymph nodes was performed on basis of the pathological results. Results:Lymph nodes with diameters of≥10 mm were evaluated, and the sensitivity and specificity of the MSCT mediastinal lymph node me-tastases were 82.4%and 92.4%, respectively. Lymph node size, primary tumor location, and visceral pleural invasion showed statistical significance in forecasting mediastinal lymph node metastases (P<0.05). Conclusion:MSCT can be used for the effective evaluation of mediastinal lymph node metastasis, lymph node size, and position of primary tumor. and visceral pleural invasion of the tumor had a higher risk of mediastinal lymph node metastasis.

Objective To observe the changes in levels of plasma leukocyte cell-derived chemnotaxin 2 (LECT2) in patients with newly diagnosed type 2 diabetes (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD),and to investigate the clinical significance.Methods A total of 137 subjects were enrolled in the study,including 50 patients with newly diagnosed T2DM complicated with NAFLD,47 patients with newly diagnosed T2DM with non-NAFLD and 40 healthy subjects.The level of plasma LECT2 was determined by ELISA.Anthropometric data and other biochemical indicators were measured in three groups.The body mass index (BMI) and waist-to-hip ratio (WHR) were calculated.Insulin resistance and pancreas β-cell function were determined by homeostasis model assessment (HOMA-IR,HOMA-％β).Results Plasma concentration of LECT2 in patients with newly diagnosed T2DM complicated with non-NAFLD was higher than that in type 2 diabetic patients with non-NAFLD [(32.95 ± 10.11 vs 29.08 ± 7.54) ng/mL,P ＜ 0.01].Plasma LECT2 levels in both groups were significantly higher than that in normal control group [(22.38 ± 4.40) ng/mL,P ＜ 0.01].Plasma LECT2 level was positively correlated with BMI,FPG,FINS,C peptide,HbA1c,GGT,TG and HOMA-IR,while negatively with HDL-C and HOMA-％ β (all P ＜ 0.01).Multivariate regression analysis showed that levels of BMI,FPG and HDL-C were important factors affecting plasma LECT2 level.Conclusions Plasma LECT2 concentration significantly elevates in patients with newly diagnosed T2DM complicated with non-NAFLD.Plasma level of LECT2 is closely correlated with insulin resistance and glycolipid metabolism.LECT2 may play an important role in the patho genesis of insulin resistance and T2DM.

Aptamers are capable of binding a wide range of biomolecular targets with high affinity and specificity. It has been widely developed for diagnostic and therapeutic purposes. Because of unique three dimensional structures and cell-membrane penetration, aptamers inhibit virus infection not only through binding specific target, such as the viral envelope, genomic site, enzyme, or other viral components, but also can be connected to each other or with siRNA jointly achieve antiviral activity. Taking human immunodeficiency virus and hepatitis C virus as examples, this paper reviewed the effects and mechanisms of aptamers on disturbing viral infection and replication steps. It may provide an insight to the development of aptamer-based new antiviral drugs.

Objective To investigate the effects of AGEs-RAGE on the apoptosis of GLUTag cells and explore the possiblc mechanism.Methods GLUTag cells treated with 0、100、200、300μg/ml of AGEs for 24h were examined for gene and protein expression of RAGE using RT-PCR and western blotting,respectively.GLUTag cells were randomly divided into four groups:control,200μg/ml AGEs,AGEs+siRNA-RAGE and AGEs+apocynin.The protein expression of p22phox、p47phox 、Bcl-2、Bax in the cells were detected with western blotting.The reactive oxygen species (ROS) levels were examined using 2'7'-dichlorodihydroflur-rescein diacetate (DCFH-DA) and the apoptosis of L cells were tested by AnnexinV-FITC/PI.Results AGEs increased thc cxpression of RAGE in a dose dependent manner.Treatment with AGEs induced a significant increase in the expression of p22phox,p47phoxand the activity of ROS,caused up-regulation of Bax and down-regulation of Bcl-2,which enhanced the apoptosis of GLUTag cells.Apocynin,the inhibitor of NADPH oxidase,prevented those responses and the effects caused by AGEs were abolished by inhibition of RAGE activity with siRNA.Conclusion AGEs positively regulate the exprcssion of NADPH oxidase-derived ROS and its down-steam signaling pathway p53/Bax by targeting RAGE,leading to the apoptosis of GLUTag cells.

Objective To investigate the blood and chest fluid level of Th17 cell and IL-17 in patients with tuberculous pleural effusion and its pathological role.Methods Flow cytometry and enzyme linked immunosorbent assay (ELISA) were used to measure the blood and chest fluid level of Th17 cell and IL-17 from 30 patients with tuberculous pleural effusion,20 patients without tuberculous pleural effusion,and 20 healthy persons.Results The blood level of Th17 cell and IL-17 wwere higher in tuberculous pleural effusion than in the other two groups(P ＜0.05).The chest fluid level of Th17 cell and IL-17 in patients with tuberculous pleural effusion were significantly higher than those in patients without tuberculous pleural effusion (P ＜ 0.05 ).The chest fluid level of Th17 cell and IL-17 in patients with and without tuberculous pleural effusion were significantly higher than that of the blood serum level.After treatment for 1,3,7 and 14 days,tbe blood serum and chest fluid level of Th17 cell and IL-17 were obviously lower.( P ＜ 0.01 ).After treatment for 1 day,the blood level of Th17 cell was obviously lower than before treatment( P ＜ 0.01 ).After treatment for 3 days,the chest fluid level of Th17 cell was obviously lower than before treatment( P ＜0.01 ).After treatment for 3 days,the blood serum level of IL-17 was obviously lower than before treatment (P ＜ 0.01 ).After treatment for 7 days,the chest fluid level of IL-17 was obviously lower than before treatment ( P ＜0.01 ).Conclusion Th17 cell and IL-17 might play an important role in the pathogenesis of tuberculous pleural effusion and they were correlated with disease progression and the therapeutic effect.