OBJECTIVE According to the development of nosocomial infection management in China,we design a series of nosocomial infection surveillance and management system,in order to work effectively in real time to control nosocomial infection outbreak,standardly collecting and analyzing the surveillance data of the critical department/area and risk factors for nosocomial infection,so a scientific management for nosocomial infection can be realized.METHODS Adopting C/S framework,the backstage supporter adopted the large-scale database of SQL SERVER 2000 edition,the front application program used PowerBuilder7 programming.The application software where collected all data about nosocomial infection information was integrated into the each work station of the information system,in order to form nosocomial infection surveillance and management network related to any department of the hospital.RESULTS System could come down with the hospital infection case which every hospital department could find to check,put in order,report,count,analyze,have a look around,inquire about,feedback,form the monitoring chart with excellent pictures and texts,and make the better prognosis of the nosocomial infection outbreak.CONCLUSIONS Beijing nosocomial infection surveillance and management system is a medical quality control system of a hospital.This software could afford scientific and standard data for the health care institution and administration to realize the primary information of nosocomial infection and make decisions.

Objective The aim of the study was to observe the change of blood lipid in uremic patients after hemodialysis or peritoneal dialysis. Methods Sera cholesterol(CH), triglyceride(TG), LDL, HDL, ApoA1, ApoB and LP(a) were measured in all patients who were dialystic treated before and after six months.Results CH, TG, LDL, HDL, ApoA1 and ApoB were significant increased in patients with uremia,but HDL, ApoA1 was significant decreased,they were apparent especially after peritoneal dialysis .However,the blood lipid levels of patients with hemodialysis or non-dialysis did not significant difference before or after treatment. Conclusions The metabolism of blood lipid in uremic patients have been changed. The disturbance will aggravate after peritoneal dialysis.We consider that therapy of reducing blood lipid is necessary in the both patients with dialysis and non- diaylsis for the uremic patients.

Objective In order to understand the mechanism of solute transperitoneal transport, we studied the relationship between IL-8 level and protein transperitoneal transport. Method 12 New Zealand White rabbits were randomly divided into experimental and control groups. 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 3?10 9 CFU staphylococcus aureus 1ml was injected into the abdominal cavity of experimental NZW rabbits and 2 5% glucose peritoneal dialysis solution (40ml/kg)contained 0.9%NaCl solution 1ml was injected into the abdominal cavity of controlling NZW rabbits. The plasma and effluent concentrations of blood creatinine ,glucose,total proteins and albumine were determined respectively, the D/P or D/Do values of creatinine , total proteins ,albumine or glucose were calculated respectively. Results The D/P ratios of creatinine, total proteins and albumin were increased significantly, while the D/Do of glucose was decreased in experimental group on different dwelling time points ,there was a significantly difference,as compared with the control group (P0 05),while there was significantly correlation after 60 mins(P

Objective To evaluate the effect of fluvastatin on the tubule function of the patients with the nephrotic syndrome. Methods Fifty two patients were divided into the experimental group and the control group at random ,all patients were treated with the standard therapies and fluvastatin was added to the experimental group.Plasma albumin,serum cretinine,serum lipid profiles,24hr urinal protein and urinary RBP were examined before treatment and after 8 weeks in all patients. Results The levels of TG,LDL,CH,urinary RBP,urinary protein after 8 weeks of the treatment were significant lowered than those before the treatment (P

OBJECTIVE To study on control measures of surgical site infection.METHODS By forward-looking survey methodology,surgical patients(including obstetrics) with surgical incision infection were investigated.RESULTS The highest infection rate(2.67%) were found in patients with surgical trauma to the largest,longest operation time,coronary artery bypass grafting.In 11 cases surgical site infections,only 1 case were given antibiotics before anesthesia,10 cases were administrated antibiotics post operation more than 4 days.The correct rate of preoperative and postoperative usage of antibiotics was only 9.09%.The size of surgical trauma,operative time,perioperative usage of antibiotics were related to the infection rate.CONCLUSIONS Target monitoing of the surgical site infection can decrease the infection rate.

Objective To observe the effect of Danhong Injection(DI)on the thyroid hormone level in patients with angina due to coronary heart disease(CHD).Methods Sixty CHD angina patients were equally randomized into the treatment group and the control group.The two groups received routine western medicine such as aspirin,angiotensin convert enzyme inhibitor(ACEI),statins,beta-blockers and nitrate esters,and the treatment group was additionally given intravenous drip transfusion of DI 20 mL,qd.Twenty days constituted one treatment course.After treatment,the therapeutic effect was evaluated and the changes of serum thyroid hormone level were observed.Results In the control group,13 patients were markedly effective,9 effective,8 ineffective,and the total effective rate was 73.3%;in the treatment group,19 patients were markedly effective,6 effective,5 ineffective,and the total effective rate was 83.3%;the difference between the two groups was significant(P

Objective To analyze the characteristics of hypertension by cross-sectional study of the aged patients with metabolic syndrome (MS). Methods The data, collected from senile population who underwent medical examination in the General Hospital of PLA in 2005, were analyzed retrospectively in order to identify the clinical features of hypertension in aged patients with MS, and the significance of hypertension, as single or one of the components of MS, the prevalence of MS, including the prevalence of MS and its component ailments, prevalence of hypertension in MS population, the constituent ratio of MS and coronary heart disease, and cerebrovascular disease in patients with hypertension. Results The prevalence of MS was 32.19%, in which 8.16% patients were having all the four metabolic abnormalities. Hypertension was the most common metabolic abnormality with a prevalence of 61.78%, which tended to increase with increasing age. The most common combination in MS was abnormalities of blood pressure, glucose and BMI, with a prevalence of 24.67%. Among the population with hypertension, 46.67% of them could be diagnosed as MS, accounting for 89.57% of all MS patients. Patients with MS but without hypertension accounted for only 10.43%. Hypertension complicated with MS or another metabolic disorder showed a high risk of cardiovascular diseases, and the prevalence was 73.52% and 72.69%, respectively. No significant difference was found in the incidence of cerebrovascular disease. The prevalence of abnormal renal function was significantly increased in the group of MS with hypertension, by 1.69 and 1.65 folds of that in the group without MS and the group only with MS and without hypertension. Conclusions There was higher prevalence of MS in the elderly people, and in about 2/3 hypertensive patients it co-existed with one of the components of MS. Hypertension was the most common disorder in MS. Patients with MS and hypertension are having a higher risk of suffering from cardiovascular diseases and abnormal renal function.

Objective To compare the accuracy of diagnostic peritoneal lavage (DPL),abdominal computed tomographic(CT) scanning,and abdominal B ultrasound for the diagnosis of blunt abdominal trauma. Methods Prospective study was made on 61 hemodynamically stable abdominal injury patients.The patients received both CT and B ultrasound before DPL ,A laparotomy was done on the condition that one of the three examinations had positive finding.The surgical findings were compared with that of the diagnostic studies. Results The sensitivity,specificity and accuracy were 97.4%,81.7% and 91.8% for DPL; 97.3% 91.3% ,and 95.1% for CT;and 92.3%,90.9% and 91.8% for B ultrasound.Although the sesitivity,specificity,and accuracy of B ultrasound in the diagnosis of blunt abdominal trauma were similar to DPL and CT, B ultrasound had more advantages than DPL and CT. Conclusions B ultrasound can replace DPL.CT as a supplementary method in the diagnosis of blunt abdominal trauma.

Objective To make sure the effect of dialysate composition on HPMCs.Methods Cell strains were subculturing.There are six groups in this experiment: Group 1(control);group 2(4.25% Glucose);group 3(1.75mmol/L Ca~(2+));group 4(1.25 mmol/L Ca~(2+));group 5(4.25% Glucose+1.75mmol/L Ca~(2+));group 6(4.25% Glucose+1.25mmol/L Ca~(2+)).The capacity of proliferation of HPMCs was assessed by MTT assay. Results Proliferation of HPMCs was inhibited in 4.25% glucose group in time dependence.Ca~(2+) induce proliferation of HPMCs,however,no effect on proliferation of HPMCs exists in different Ca~(2+) group.Conclusion High glucose can inhibit cell proliferation;Ca~(2+)(1.75mmol/L,1.25mmol/L) can promote the proliferation and 1.25mmol/L is better.

Objective To study the function of the nano-molecular polyamide-amine (PAMAM) as microRNA(miR) carrier targeting gastric adenocarcinoma, and the foundation of developing an efficient delivery of small molecule drugs tar-geting gastric cancer thereof. Methods The folic acid (FA)/PAMAM comoles compound was prepared by dialysis method. After transfection of miR-7 or liposomes into SGC-7901 cell line, fluorescence microscope was used to detect the gene trans-fect efficiency. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the miR-7 level. The immu-nocytochemistry assay was used to test the protein expressions of epidermal growth factor receptor (EGFR), protein kinase B (PKB) and proliferating cell nuclearantigen (PCNA). The transwell system was utilized to explore the migration ability of tu-mor cells. Results Compared with liposme, FA/PAMAN complex compound can significantly improve the level of miR-7 in SGC-7901 cells,reduce the protein levels of EGFR, PKB and PCNA in SGC-7901 cells, and also reduce the percentage of cancer cell migration (P<0.05). Conclusion PAMAM can effectively transfect miR into gastric cancer cells, which is expected to become an efficient delivery of small molecule drugs.