1.Research of in vitro Dissolution of Berberine Solid Preparation Based on Biological Activity
China Pharmacist 2016;19(4):642-646
Objective:To explore the chemical and biological assay pattern for the quality control and evaluation of Chinese mate-ria medica solid preparations ( CMMSP) . Methods:Compound berberine tablets were used as the model drug, the inhibition against sensitive strains of the dissolution solution at different time points was studied with water as the dissolution medium, and the cumulative dissolution of compound berberine tablets was obtained based on the bacterial inhibitory rate. The dissolution of hydrochloric acid ber-berine was also determined by HPLC, and a f2 similar factor method was used to evaluate the relevance of the two methods. Results:The antimicrobial test showed that berberine was sensitive to escherichia coli,staphylococcus aureus, bacillus subtilis, micrococcus luteus and candida albicans etc, and among them, the antibacterial circle edge of bacillus subtilis was clear with higher sensitivity. The disso-lution curve of compound berberine tablets determined by HPLC as the reference, the similarity factor f2 of bio-dissolution curve of com-pound berberine tablets from 7 different manufactures was greater than 50, which suggested that the dissolution determination method based on biological titer determination could comprehensively reflect the bioavailability of compound berberine tablets. Conclusion:The biological activity evaluation method for dissolution is expected to be one of the effective means for in vitro dissolution test of CMMSP.
2.Flash Extraction Process Optimization of Chlorogenic Acid and Luteoloside from Lonicera Japonica by Box-Behnken Design and Response Surface Methodology
Shufen HE ; Junhong XU ; Yingguang OUYANG ; Zhenqiu CHEN
China Pharmacist 2017;20(8):1352-1355
Objective: To optimize the flash extraction process of the active ingredients from Lonicera japonica by Box-Behnken design and response surface methodology.Methods: Flash extraction was used.With the concentration of ethanol,solid-liquid ratio and extraction time as the main influencing factors and the overall normalization value of the transfer rates of chorogenic acid and luteoloside as the evaluation index, Box-behnken design was performed to screen out the optimal extraction conditions.The mathematics relationship between the overall normalization value and the independent variables was established by multiple linear regression and binomial fitting, and response surface methodology was used to predict the optimal process conditions.Results: The optimal extraction conditions were determined as follows: the ethathol concentration was 66.92% ,the solid-liquid ratio was 18.82 ,the extraction duration was 1.20 min and extracted only once.Under the above conditions, the extraction rate of chloragenic acid and total luteoloside was 92.87% and 87.55% , respectively.Conclusion: The optimized technology is simple,economic and practical,which can provide new ideas for the rapid extraction and quality control of active ingredients from Lonicera japonica .
3.Revascularization of arteriosclerosis obliterans of the lower extremity
Kunmei GONG ; Le XIAO ; Kunhua WANG ; Yongxue ZHANG ; Yiming OUYANG ; Ping LING ; Yingguang HUANG ; Linhai LI ; Yaxin LONG ; Jian ZHANG ; Yu ZHU
Chinese Journal of General Surgery 2009;24(6):459-462
Objective To evaluate traditional surgical treatment, intraluminal strategy and hybrid operation on revascularization of atherosclerosis obliterans (ASO) of the lower extremity. Methods Clinical data of 197 ASO cases receiving revascularization from January, 1998 to December, 2008 were retrospectively analyzed. Seventy-seven cases underwent surgical treatment, 82 cases received intraluminal therapy, and 38 cases were treated by hybrid operation. The indications, clinical effect, complication and perioperational mortality of these three strategies were evaluated. Results 71% patients (164 cases) were followed up from 2 to 112 months. Surgical and intraluminal method had no statistical difference on long-term patency of aortic-iliac and femoral-popliteal artery (57% vs. 51%;48% vs. 42%). Hybrid procedure led to higher patency on multi-level lesion and concurrent thrombosis. The complications after surgery was higher than intraluminal on aortic- iliac and femoral-popliteal artery (31% vs. 12%;31% vs. 11%), and higher than intraluminal and hybrid on multi-level lesion (36% vs. 12% vs. 15%). The perioperative mortality of surgical group was 1.5% and 2.0% on aortic-iliac and multilevel lesion and 0% on other site;and that of intraluminal and hybrid procedure was 0%. Conclusion For aortic-iliac and femoral-popliteal artery revascularization, surgery was preferred in cases of long occlusive lesion and intervention was preferred for cases with short non-occlusive lesion. Hybrid procedure was the best for multi-level and concurrent thrombosis.
4.Study of cotransfection of B7-1 gene and CD1_D gene in pancreatic carcinoma cell and its anti-tumor responses in mice
Kunhua WANG ; Kunmei GONG ; Yongxue ZHANG ; Ming ZHONG ; Yiming OUYANG ; Ping LIN ; Yingguang HUANG ; Jian ZHANG ; Yu ZHU ; Weijun LIU ; Xirong ZHAO
Chinese Journal of General Surgery 1994;0(05):-
Objective To study the cotransfection mB7-1 and mCD1D gene into pancreatic cancer cells of rats and to observe its anti-tmor responses.Methods Recombinant retroviral vectors expressing mB7-1and mCD1D gene were packaged into GP2-293 cell lines and transfected.The expressions of mB7-1 and mCD1D were detected with PCR and Western blot.The positive cells of mB7-1 and mCD1D were used to induce the anti-tumor immunity in vitro.Results Anti-tumor immunity was induced after B7-1 and CD1D positive cells were coinoculated in syngeneic mice.Furthermore,the growth of tumor was inhibited.Conclusions Cotransfection of B7-1 and CD1D could induce anti-tumor effect.This study provide a foundation for the application of B7-1 and CD1D gene therapy in tumor.