【Objective】To observe the influence of temperature and perfusate modifier on in-vitro mass transfer coefficient of microdialysis with sinomenine as a model drug.【Methods】The in-vitro relative recovery(RR) was assayed by HPLC at different temperatures and in perfusate including different concentrations of alcohol,and then the mass transfer coefficient was calculated.【Results】In-vitro RR increased with the temperature and the concentrations of alcohol,so did the mass transfer coefficient.【Conclusion】Temperature and alcohol at different concentrations have an effect on RR and mass transfer coefficient of microdialysis with sinomenine as a model drug.

AIM: The transdermal absorption data of sinomenine patch was fitted by grey model GM(1,1) and compared with other models. METHODS: The transdermal absorption experiment was carried out with Franz diffusion cell and the GM(1,1) was established by accumulation generation operator (AGO). RESULTS: The fitting precision was compared by using mean relative error and grey absolute correlation degree as evaluation criterion. CONCLUSION: The fitting precision of GM(1,1) is as high as acceptable.

Objective To explore the value of combined detection of carbohydrate antigen 125(CA125),human epididymis secre-tory protein 4 (HE4)and plasma D-dimer in early diagnosis of ovarian cancer.Methods Fifty-six patients with ovarian cancer and 50 patients with benign ovarian tumors in the gynecology department of our hospital from January 2014 to June 2016 were selected. Contemporaneous 50 women undergoing healthy physical examination were selected.The chemiluminescence method was adopted to determine serum CA125 and HE4 levels,and the immune turbidimetry method was adopted to detect plasma D-dimmer level.Their early diagnostic values for ovarian cancer were comparatively analyzed.Results The serum CA125 and HE4 levels and plasma D-di-mer level in the cancer group were significantly higher than those in the control group with statistical difference(P<0.05),moreo-ver HE4 had higher sensitivity(89.28%)and higher specificity (88.00%)for detecting ovarian cancer.In the 3-index combined de-tection,sensitivity and specificity were greatly improved.Conclusion The combined detection of CA125,HE4 and plasma D- dimer can improve the accuracy of early diagnosis of ovarian cancer.

miniature swines were randomly divided into irradiation group ( n =7) and control group ( n =8). Immediately after balloon overstretch injury to LAD, radioactive liquid perfused balloon irradiation was performed at the target segment; radioactive dose was 24Gy. 35 days after the operation, the target segments were harvested to perform histologic and morphologic study (HE, MS, VVG) and immunohistochemical study (PCNA, ? smooth muscle actin). Results showed that the lumen area was significantly larger, the neointima area and vascular stenosis level were smaller, and less PCNA positive cells were present in the vascular wall in the irradiation group than in the control group ( P all

Three problems of treatment based on syndrome differentiation, TBSD are discussed here. Firstly, in allusion to the diversity of the concept of TBSD, the due medical concept of it are discussed and analyzed. Hence, the actual difference and sameness, the due relationship as well as the principle of management between syndrome differentiation and disease differentiation are fully compared. Finally, the concrete operation procedure of syndrome differentiation and disease differentiation are expounded. The author detailedly introduced particular cognition to standardized operation of TBSD attained from Yao Hesheng, a famous physician in Jiangxi Province.

Objective To study the effect of skin stratum corneum on transdermal absorption characteristic of Sinomenine Liposome Patch. Methods The normal and stripped stratum corneum skin of back and abdomen was used to study the transdermal absorption characteristic by Franz method. The transdermal absorption sample was taken at the preset sampling time point. The sample content was determined by HPLC. The cumulative transdermal amount of per unit area was fitted with sampling time point, the transdermal rate constant (J value) was selected as comparative standard. Results The transdermal rate constant (J) of skin with stratum corneum stripped increased obviously than that of the normal skin, so was the released rate constant. Conclusion The skin stratum corneum has an notable impact on transdermal absorption characteristic of Sinomenine Liposome Patch. When the skin of experimental animal being prepared, the integrality should be paid more attention.

AIM: To investigate the feasibility to inhibit the expression of MHCⅡ by special siRNA targeting class Ⅱ major histocompatibility complex (MHC Ⅱ) transactivator (CⅡTA), which might regulate MHC Ⅱexpression for suppressing immune rejection. METHODS: Five different siRNA were designed, synthesized and transfected into freshly isolated rat corneal keratocytes. At 24 hours posttransfection, the changes of MHC Ⅱexpression were detected by flowcytometry, and the mRNA abundance of CⅡTA and MHC Ⅱ was measured by FQ-PCR after inducing with recombinant rat interferon-gamma (IFN-?). RESULTS: Different siRNA showed different reduction in MHC Ⅱ and CⅡTA expression compared with the control (P

Objective To isolate and identify the putative Japanese encephalitis virus (JEV) re-ceptors from C6/36 and Vero cells. Methods Molecules binding with JEV were isolated from C6/36 and Vero cells by co-immunoprecipitation (Co-IP) approach, identified by mass spectrometry, and detected by Western blot. The location of putative JEV receptor on cells membrane and the binding with JEV were ob-served by laser scanning confocal microscopy (LCM). Results Several molecules binding with JEV were isolated from C6/36 and Vero cells by Co-IP, and only one molecule was identified as heat shock cognate 70 (HSC70) by mass spectrometry. Antibody against HSC'70 was able to detect a 74 ×103 protein isolated by Co-IP from C6/36 and Vero cells membrane in Western blot assays. It was observed by LSCM that when JEV attached on the surface of C6/36 cells, JEV and HSCT0 protein were co-localization. Conclusion 74 x 103 molecular identified as HSC70 protein from C6/36 cells may be JEV receptor.

Objective To observe the expression of caspase-3 and transposition of Omi/HtrA2 in H9C2 by erythropoietin and/or Omi/HtrA2 silencing to explore the related anti-apoptotic mechanisms.Methods The cultured H9C2 cardiomyocytes were divided into control group,H/R group (anoxia 2 h and re-oxygenation 24 h), and different concentrations of EPO treatment groups.The release rate of lactate dehydrogenase (LDH)in cell supernatant was measured in each group.Expressions of cleaved caspase-3 and Omi/HtrA2 were measured by Western blot;then the transposition of Omi/HtrA2 between cytoplasm and mitochondria was observed.Specific siRNA interfering fragment was transfected into H9C2 cardiomyocytes by liposome method.Its silencing effect on Omi/HtrA2 was measured by RT-PCR and Western blot.The survival rate,release rate and expression of cleaved caspase-3 were measured.And the expression of Omi/HtrA2 was measured in cytoplasm and mitochondria in H9C2 (transposition of Omi/HtrA2 ).Results Compared with H/R group,the release of LDH and expression of cleaved caspase-3 were decreased; the transposition of Omi/HtrA2 from mitochondria to cytoplasm in H/R treatment groups was increased compared with control group,while that in EPO (20 IU/mL)group decreased.si-HtrA2 group transfected with siRNA showed a decreased release of LDH and expression of cleaved caspase-3 with all significant variances (P <0.05).Conclusion EPO exerts a cytoprotective effect by inhibiting the transposition of Omi/HtrA2 and hence the activation of caspases-3 pathway.

Objective Screening the potential lncRNAs of KIAA1456 inhibiting ovarian cancer cells.MethodsImmunofluorescence was used to detect the expression of KIAA1456 in HO8910PM ovarian cancer cell.Microarray was performed to identify differently expressed lncRNAs in HO8910PM ovarian cancer cells overexpressed KIAA1456 as compared with the normal control group. The interesting candidate lncRNAs were verified by real-time quantitative PCR.Constructing RNAi lentiviral vector targeting KIAA1456 gene in HO8910PM ovarian cancer cells, RT-qPCR and Western blot was performed to measure the interference efficiency.Differentially expressed lncRNAs were validated by RT-qPCR after KIAA1456 knock-down.Results KIAA1456 mainly expressed in nucleus of HO8910PM cell.A total of 654 differently expressed lncRNAs were identified in the KIAA1456-overexpressed group compared with the control group with a set filter fold-change ≥1.2, the most aberrantly expressed lncRNAsincluding down-regulated gene SSX8 and up-regulated genes SNORD14D,SNORA26,lncRNA-ENST00000384488.The results of the RT-qPCR were consistent with the data from the microarray.The shRNA interference vectors targeting the KIAA1456 gene were successfully constructed, SSX8 was up-regulated, and SNORD14D,SNORA26,lncRNA-ENST00000384488 were down-regulated after knockdown the expression of KIAA1456 in the HO8910/PM ovarian cancer cells.Conclusions KIAA1456 inhibits proliferation of ovarian cancer by regulating correlative lncRNAs, which may provide a new target for the diagnosis and treatment of ovarian cancer.