BACKGROUND: Electrostatic layer-by-layer (LbL) self-assembly is a simple, effective and novel approach to carrying macromolecules into microcapsule carriers.OBJECTIVE: To prepare chitosan (CS) microcapsules to control the releasing of heparin (Hep) by LbL.METHODS: CS microspheres were fabricated by a sodium sulfate-based precipitation process and then used as positively charged templates for polyelectrolyte multilayer coatings to carry heparin by the LbL self-assembly technique. The polycation was CS and the polyanion was Hep. The microcapsule was formed via coating {CS/Hep}3 on CS hydrogel template. The microcapsules composed of {CS/ Hep}3 on CS hydrogel templates were confirmed by fluorescence inverted microscopy, confocal laser scanning microscopy and laser particle size analysis. The assembly of polyelectrolyte layers on CS hydrogel microspheres was monitored by Zeta Potential Analyzer.RESULTS AND CONCLUSION: The resulting microcapsules were about 1 μm in average diameter, and allowed spontaneous loading of heparin through electrostatic interaction, with the encapsulation efficiency and carrying capacity of 83.8% and 3.05%, respectively.

AIM: Coding microsphere suspension chip is a novel protein chip technology, which is considered as the developing direction of medical tests. This study investigated the feasibility of coding microsphere suspension chip to detect coronary heart disease (CHD)-associated protein factors. METHODS: The serum samples of 30 patients with CHD were collected including 19 males and 11 females, with average age of (63?10) years in the Department of Cardiology, Zhujiang Hospital of Southern Medical University from August to September 2007. Patients did not have hepatic or renal dysfunction, cerebrovascular accident, acute infection or any anticoagulation medication. The regional ethics committee approved the study protocol and the informed consents were obtained from all subjects. The integrated detection method was established by coding microsphere suspension chip to detect CHD-associated protein factors including high sensitivity C reactive protein (hs-CRP), lipoprotein a (Lpa) and adiponectin (ADPN). Meanwhile, these three factors from 30 CHD cases were detected by the enzyme-linked immunosorbent assay (ELISA). The detection results by both methods were compared. RESULTS: The serum samples of 30 CHD cases were all included in final analysis. The detection results by coding microsphere suspension chip integrated detection method were hs-CRP (2.54?1.97) mg/L, Lpa (102.41?76.74) mg/L and ADPN (15.43? 9.87) mg/L. The detection results by ELISA were hs-CRP (3.93?2.52) mg/L, Lpa (208.63?81.23) mg/L, and ADPN (16.63? 8.21) mg/L. The detection results by coding microsphere suspension chip integrated detection method were correlated significantly to that of ELISA (hs-CRP: r=0.979, P

Objective:To investigate the curative effect and prognostic factors of primary liver cancer(PHC) treated with radical resection combined with transcatheter arterial chemoembolization (TACE).Methods:A retrospective analysis was used to analyze the clinical data of 136 cases of PHC patients treated in Qianjiang Central Hospital of Hubei Province from January 2008 to January 2012,the 86 cases received radical resection and postoperative adjuvant TACE treatment of patients as the observation group,and the remaining 50 cases only with radical resection in the treatment of patients were selected as control group.After 6 months of treatment,the level of tumor markers Carcinoembryonic antigen(CEA),alpha fetoprotein(AFP),CA199 and alanine aminotransferase(ALT) in two groups were observed and compared.The survival status and prognostic factors of the two groups were compared.Results:After 6 months of treatment,the leve of tumor markers CEA,AFP,CA199 and ALT in the observation group were significantly lower than those in the control group(P＜0.05);The 1-year,2-year,and 3-year survival rate of the observation group were significantly higher than those in the control group;Univariate analysis showed that the differentiation degree,tumor size,lymph node metastasis,vascular tumor thrombus,CA199 concentration factors and the observation group patients (radical resection and postoperative adjuvant TACE) postoperative survival period (P＜0.05);Further multivariate analysis showed that the degree of differentiation,lymph node metastasis and CA199 concentration were the important factors influencing the prognosis of patients with TACE after radical resection (P＜0.05).Conclusion:Radical resection combined with TACE in the treatment of PHC has well clinical curative effect,which can effectively improve the postoperative long-term survival rate.Degree of differentiation,lymph node metastasis,and CA199 concentration are important factors impacting the radical resection operation adjuvant TACE prognosis.

Objective This study is to discuss the effects of cognitive therapy on life quality of rural breast cancer patients during chemotherapy in order to offer evidence for choosing the appropriate therapeutic measures.Methods 120 rural breast cancer patients were equally divided into the intervention group and the control group at random.The usual care plus cognitive treatment was given to the patients in the intervention group for 6 months,while only the usual care after chemotherapy was given to the control group.The quality of life assessments were carried out to both groups on the 5th day during hospitalization,and the first,third and 6th month during recheck period of time,respectively.The treatment effect was observed.Results There were statistical significant difference of the total scire of life quality assessment on the 5th day,the first,third and 6th month,respectively,between the intervention group and the control one after cognitive intervention.The downtrend of total score of quality of life in the intervention group after cognitive intervention was more significant than the control group,which indicated the quality of life of the intervention group was better than the control group. Conclusions Cognitive therapy is an efficient method to enhance the quality of life of rural breast cancer patients during chemotherapy.

[Objective] To observe the counteractive action of cyclovirobuxinum D (Cvb-D) for myocardial ischemia and to explore its possible mechanism. [Methods] Sixty SD rats were randomized into blank control group, Cvb-D groups in the dosage of 2.2, 1.1 and 0.55 mg? kg-1 ? d-1 respectively, isosorbide dinitrate group (25 mg? kg-1 ?d-1). The blank control group was treated with distilled water and the other groups were treated with the corresponding drugs by gavage according to the experimental design for 30 days. One hour after the last medication, pituitrin (1.5 U/kg) was injected into the caudal vein of the rats except the blank control to induce coronary artery spasticity myocardial ischemia. After that, electrocardiogram (ECG) was recorded, plasma superoxide dismutase (SOD) activity, malonyldialdehyde (MDA), lactate dehydrogenase (LDH) and phosphocreatine kinase ( CPK) levels were detected. [ Results ] The incidence of ischemic changes in ECG was decreased, plasma SOD activity increased and MDA, LDH and CPK levels lowered in Cvb-D groups as compared with those in the model group (P

This article analyzes the current characteristic of cardiology teaching.In light of advantages of network education,it discusses the prospect of network-based education and some problems.

Objective To analyze the characteristics of mixed ground glass nodular adenocarcinoma in multi-slice spiral CT(MSCT) and its diagnostic value.Methods This study was prospective study,120 patients with mixed glass nodules lung adenocarcinoma were classified as lung adenocarcinoma in the People's Hospital of Xinzhou from January 2014 to December 2016.178 cases of mixed glass nodular pneumonitis were classified as pneumonia.The MSCT signs were compared between the two groups.The imaging features of medullographic nodules with different pathologic types of lung adenocarcinoma by grouping of patients with lung adenocarcinoma were compared.Results In the lung adenocarcinoma group,the dizzy uneven thickness rate (61.67％),leaf rate (60.00％),clear boundary rate (80.83 ％),blood vessels,gather tax rate (64.17 ％),pleural indentation rate (61.67 ％) were significantly higher than those in the pneumonia group(35.96％,35.39％,41.01％,44.94％,41.57％,x2 =8.69,12.47,33.64,7.62,7.97,all P ＜ 0.01).The mixed grinding glass nodules of IAC,MIA and MIS in size,number,shape,position and density were significantly different(all P ＜ 0.05).The IAC type mixed grinding glass nodules was irregular single nodules (volume generally ≥5 mm),common deviation centricity growth,and with blood vessels.The AIS type mixed grinding glass nodule was single nodule (volume generally ≥5 mm),formed a polygon or dotted,common growth of centricity,vascular density was close to the same level.The volume of the MIA type mixed grinding hyaline nodules was most ＜ 5 mm,common to multiple,with oval or round most,common to deviate from the centricity growth,lower density than plane tree blood vessels.Conclusion MSCT is helpful in the differential diagnosis of mixed ground glass nodules of lung adenocarcinoma and mixed ground glass nodular inflammatory lesions,and pathological types have leaded to the identification of mixed ground glass nodules of lung adenocarcinoma MSCT,which has higher application value.

Objective To investigate how to improve safety of insulin injection in type 2 diabetes patients by“insulin pen”through the rotating insulin weekly abdominal injection card. Method All the inpatients with type 2 diabetes in the Department of 3rd Internal Medicine from January to September, 2013 were enrolled in this study. Insulin Injection Card group was composed of 66 type 2 diabetes patients who were admitted from June to September, while control group was composed of 62 type 2 diabetes patients admitted from January to April. The difference between Insulin Injection Card group and control group was that the former group used the rotating insulin abdominal injection card weekly which was designed by ourselves, while the control group patients did not use the card. Every participant of both groups was evaluated at 2 weeks of treatment by percentage of patients having absence of Safety standard of insulin injection, and at the end of 3 months of treatment by percentage of patients attaining the therapeutic targets and incidence of adverse events. Result Compared with the control group, patients in Insulin Injection Card group had lower proportion of absence of Safety standard for insulin injection. The scores of rotation injection site in the two groups was 0.59±0.14 and 2.67±0.21(P<0.01). The rate of FPG target achievement of patients in Insulin Injection Card group (84.9%) was higher than that of the control group (61.3%)(P<0.01), the rate of HbA1c target achievement of patients in Insulin Injection Card group (78.8%) was higher than that of the control group (56.5%)(P<0.01). Patients in Insulin Injection Card group had lower incidence of local adverse events (1.5%) compared with the control group (12.9%)(P<0.05). Conclusion Use of the rotating insulin weekly abdominal injection card that was designed by the diabetes health management Quality Control Group in our department showed good effect in achieving therapeutic targets (including HBA1C) and decreasing safety standard deficiency of insulin injection and adverse events.

Objective To investigate the effect of fluid resuscitation on hemodynamics in dogs with hemorrhagic shock at the initial stage of getting high altitude. Method he models of severe hemohagic shock were established in 13 mongrel dogs after they were brought to the high altitude a/ea and were mndondvvided inIo 3 group: LR group(n=3),6% HES group(n=5)and control group(n=5).The dogs in LR group wefe intravenously infused with lactated Ringers solution in 1.5 limes exsanguinated volume;those in 6% HES group were given hydroxyethyl starch in equal volume of exsanquination. A drop of solution waft noven to dogs in con,trol group.One hour after restritation,lactated Ringers solution waft infused at 5 Ilps as maintenance dose.The hemodynmnic variables were recorded.Results All animals in control group died two hour later.In LR group,the hemedynamie variables including MAP,CO,PAWP,CVP,LVSWI and RVSWI two hours after infiion were signifieantly higher than those.one hour after infusion(P<0.05),while HR,SVRd PVR were significantly lower(P<0.05).In dogs of 6% HES group,CO,PAP,PAWP,CVP,SVR and PVR two hours after infusion were significantly higher than those one hour after infusion(P<0.05),but MAP.HR.CI,LVSWI and RVSWI were not significantly different(P>0.05).Conclusions All of three doith hen rhagic shock would die(3/3)at the initial stage of being brought to high altitude alles without fluid resuscitation. Fluid resuscitation with LR solution 1.5 times the exsanquinated volume was effective and safe. The infusion of 6% hydroxyethyl starch at equal volume of exsanqination may easily cause heart failure or pulmonary edema.

Objective To study the influence of hemodialysis on inflammatory state and immune function by analyzing the change of serum protein components in uremic patients before and after hemodialysis.Methods 75 cases of uremic patients confirmed by the Nephrology from October 2013 to May 2014 were selected as the observation group,and 15 healthy volun-teers at the same time as the control group.Then the serum protein electrophoresis pattern of observation group beford the first hemodialysis,after the first hemodialysis,after one month’s treatment and control group were compared with each oth-er.Results In the observation group before and after the first hemodialysis,the ALB levels were lower,α1 and α2 globulin levels were higher than those in control group.There was a statistically significant between the observation group before and after the first hemodialysis and control group (P <0.001).After the first hemodialysis,there were differences inα1 globulin levels compared with before the first hemodialysis (P <0.01).ALB was no significant difference after one month’s hemodi-alysis compared with before the first hemodialysis,andα1,α2 globulin were significantly reduced and the difference was sta-tistically significant (P <0.001),ALB was lower than the control group and was statistically significant (P <0.001).After one month’s hemodialysis,the levels ofγglobulin were higher than those in control group,before the first hemodialysis and after the first hemodialysis.There were significant differences (P <0.001 or P <0.01).Conclusion Regular and effective hemodialysis can improve inflammatory state and immune function of uremic patients.