Objective: To discuss the feasibility of using tissue engineered bone to repair large chest wall defects. Methods: Skeletal chest wall defects were reconstructed by tissue engineered bones in five dogs. These tissue engineered bones were constructed by seeding autologous mesenchymal stem cell (MSC) onto xenogenic acellular porcine bone matrix (APBM), a biodegradable scaffold. Within every regenerated bone was and assistant nutrition catheter designed to keep the cell living before reformation of blood supply. This catherter could also maintain the bone regeneration microenvironment through bone morphogenetic protein (BMP) and other growth factors delivered every day. Apparently diminished repair potential may be prevented by periodic administration of MACs. Results: Three-months follow-up showed that sufficient chest wall stability was obtained, and no postoperative complication occurred such as flail chest, infection. Conclusion: Tissue engineered bone is a perfect biomaterial for chest wall defect reconstruction.

OBJECTIVETo investigate the feasibility of the composite transplantation of 1:3 meshed split-thickness autograft and acellular heterologous (porcine) dermal matrix.

METHODS9 inpatients with full thickness skin burn or hypertrophic scar were selected in this study. After the eschar or scar was excised, the wound was covered with acellular heterologous dermal matrix. Then the meshed (1:3) split-thickness autologous skin sheet was grafted on the dermal matrix. Before dressing up, the radiated pigskin was placed on the composite transplants.

RESULTSThe composite transplantation was successfully used in 9 cases. The meshed split-thickness autograft was expanded 3 times and covered the dermal matrix tightly. The clinical results of the composite transplantation were similar to that of intermediate split thickness skin graft or full thickness skin graft.

CONCLUSIONThe composite transplantation of meshed (1:3) split-thickness autograft and acellular heterologous (porcine) dermal matrix allowed the expansion of the autologous skin sheet to 3 times. The clinical results were similar to that of intermediate split thickness skin graft or full thickness skin graft.

Adolescent ; Adult ; Animals ; Burns ; pathology ; surgery ; Child ; Dermatologic Surgical Procedures ; Dermis ; transplantation ; Female ; Graft Survival ; physiology ; Humans ; Male ; Middle Aged ; Skin ; pathology ; Skin Transplantation ; methods ; Swine ; Transplantation, Autologous ; Transplantation, Heterologous ; Wound Healing ; physiology

OBJECTIVETo present the clinical application of the meshed acellular dermis xenograft with scalp thin split-thickness skin autograft.

METHODSThe meshed acellular dermis xenograft (pigskin) was placed on the granulation or defects after scar resection. Four or five days afterwards, scalp thin split-thickness skin was transplanted. A total of 15 patients with 25 wounds were treated using this technique. The survival rates and quality of the grafts were observed.

RESULTSThe survival rate of the meshed acellular dermis xenograft was (96.40 +/- 2.60)% and the scalp thin split-thickness skin autograft was (97.44 +/- 3.50)%. All grafts showed normal skin-alike color and elastic and smooth texture.

CONCLUSIONThe combined use of meshed acellular dermis xenograft and scalp skin autograft demonstrated an ideal way for the repair of full-thickness skin burn or defects from scar resection. The scalp can provide thin skin graft repeatedly without influence of the hair.

Adolescent ; Adult ; Animals ; Burns ; pathology ; surgery ; Child ; Child, Preschool ; Dermatologic Surgical Procedures ; Dermis ; transplantation ; Female ; Humans ; Male ; Middle Aged ; Skin ; pathology ; Skin Transplantation ; methods ; Swine ; Transplantation, Autologous ; Transplantation, Heterologous ; Wound Healing

OBJECTIVETo investigate the dynamic process of the inflammatory response and the profile of Th1/Th2 cytokines after xenogenic acellular dermal matrix (ADM) transplantation with thin split-thickness skin autograft overlay.

METHODSSD rats were used in the study. In the control group, thin split-thickness skin autograft (STSG) was transplanted in the full-thickness skin defect of the SD rats; in the experimental group, the xenogenic acellular dermal matrix combined with thin split-thickness skin autograft was transplanted. The inflammatory response was examined histologically and Th1/Th2 cytokine mRNA expression in skin grafts was determined by reverse transcription-polymerase chain reaction.

RESULTSInflammatory reaction was induced by ADM at the early stage of transplantation and decreased gradually. Th2 cytokine mRNA expression was higher in the ADM group than that of the control group whereas the Th1 cytokine mRNA expression was undetected in both groups.

CONCLUSIONXenogenic acellular dermal matrix is immunogenic. The increased expression of Th2 cytokines may be related to the humoral immune responses and the absence of ADM graft rejection.

Animals ; Cytokines ; genetics ; Dermis ; immunology ; transplantation ; Gene Expression ; Inflammation ; immunology ; Interferon-gamma ; genetics ; Interleukin-2 ; genetics ; Male ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Skin Transplantation ; methods ; Swine ; Transplantation, Autologous ; Transplantation, Heterologous

OBJECTIVETo explore the biological reaction of swine acellular dermal matrix as a filling material for body surface deformity.

METHODSXenogenic acellular dermal matrix was implanted into the hypoderm of rabbits. The implants were measured and observed with pathological and transmission electron microscopic technique at regular times. The results were compared with implantation of autologous dermis and swine dermis.

RESULTSThe implanted xenogenic acellular dermal matrix and autologous dermis had slight inflammatory reaction. At 2 weeks there were fibroblasts and capillaries in the implants. The absorptivity of the xenogenic acellular dermal matrix was lower than the swine dermis. The xenogenic acellular dermal matrix was used in 2 clinical cases and obtained good result.

CONCLUSIONThe xenogenic acellular dermal matrix is an ideal material with good compatibility feature and low absorptivity.

Animals ; Biocompatible Materials ; metabolism ; Cleft Lip ; surgery ; Dermis ; physiology ; transplantation ; ultrastructure ; Female ; Forearm ; abnormalities ; surgery ; Graft Survival ; physiology ; Humans ; Male ; Microscopy, Electron ; Rabbits ; Skin Transplantation ; methods ; Subcutaneous Tissue ; surgery ; Swine ; Transplantation, Heterologous