Objective To investigate the effects of variable sample preparation about sialy LewisA/X on the surface of colon carcinoma cells.Methods Human colon carcinoma cell suspension was prepared by using EDTA,trypsin,cell scraper,trypsin-EDTA and trypsin-cell scraper.Cell counts and sialy-LewisA/X on the surface of colon carcinoma cells were detected by flow cytometry.Results Trypsin made fluorescence intensity of sialy-LewisA/X on the surface of colon carcinoma cells become weaken.Fluorescence intensity of sialy-LewisA/X had no significant deviation among EDTA,cell scraper and trypsin-cell scraper.Cell counts were multitude in EDTA group or trypsin-cell scraper group.Conclusion Trypsin-cell scraper is a available approach on preparing cell suspension of adherent cell.

Objective To observe the effect of external use of Curcuma oil on the hyperplasia of mammary glands in rats and explore the mechanism. Methods Sixty female Wistar rats were randomly divided into following groups:normal con-trol group, model control group, Sanjierubi plaster group, low-, medium- and high-dose curcuma oil emulsion groups ( n=10 each).The models of hyperplasia of mammary glands were established by intramuscular injection of estradiol benzoate and proges-terone into the medial part of the rat hind limb.Different doses of medicines were given for 4 consecutive weeks.Enzyme-linked im-munosorbent assay, HE staining and immunohistochemistry were used to investigate the action mechanism of curcuma oil emulsion against mammary gland hyperplasia. Results Curcuma oil emulsion had preventive and therapeutic effects on the hyperplasia of mammary glands.The diameter of breasts was significantly reduced, the body weight restored, serum estradiol, follicle-stimulating hormone and prolactin levels profoundly decreased, progesterone, testosterone and luteinizing hormone levels markedly increased and the number and diameter of lobular acini obviously reduced in high-dose curcuma oil emulsion group when compared with those in model control group (P<0.05 or P<0.01). Conclusion Curcuma oil emulsion can remarkably improve the disturb-ance of serum hormones and inhibit the occurrence of hyperplasia of mammary glands.

Objective To optimize the extraction process of anti-hepatic fibrosis water-soluble component from Carthamus tinctorius L. via the orthogonal method. Methods The influences of type and content of solvent,extraction time,and extraction frequency on processing were investigated by assessing the content of hydroxysafflor yellow A,yield of extraction and anti-hepatic fibrosis potency via the high performance liquid chromatography and enzyme-linked immunosorbent assay by L9(3)4 orthogonal test. Results The optimum extracting condition for the anti-hepatic fibrosis water-soluble component from carthamus tinctorius L. was as follows: extracting with 10 times the volume of 10% ethanol for twice, 0. 5 h in each. Conclusion The process is scientific and feasible, which serves as a guideline for the production of the anti-hepatic fibrosis water-soluble components.

Objective To prepare the hydroxysafflor yellow A (HSY-A)enteric coating pellets,and investigate in vitro release and in vivo pharmacokinetic characteristics.Methods HSY-A enteric coating pellets were prepared by extrude-rounding and fluid bed technique.The micromeritic characteristics,the factors affecting the release properties of enteric coating pellets the release mechanism were explored,and the in vivo pharmacokinetic behaviors were also evaluated.Results The in vitro release behavior of HSY-A from enteric coating pellets could be described by Ritger-peppas equation,and fit diffusion mechanism,in vivo pharmacokinetic test confirmed the argument that pellets have good acid residence and enteric properties. Conclusion HSY-A enteric coating pellets have been successfully prepared and the expected release properties is achieved in the study.

Objective: To observe the effect of Tongluo Jiunao oral solution on learning and memory and the expression of AChE in hippocampus of AD rats induced by ?-amyloid1-40.Methods: 140 SD rats were divided into blank control group(normal group),pseudo-operation control group,negtive control group(model group),positive control group and experiment group(48、24、12mg?kg-1?d-1)at random.The model of AD were established by injecting ?-amyloid1-40 into bilateral hippocampus.After 4 weeks,Morris Water Maze was used to determine the ability of learning and memory.Immunohistochemical method and image analysis techniques were used to determine the expression of AChE in Hippocampus.Results: Compared with normal group,in place navigation test,the mean escape latencies of the model group increased obviously,and in spatial probe test,the frequency of passing through the platform of the model group decreased obviously(P