Objective To investigate the ability in the activities of daily living (ADL) of children with hemophilia and the factors which influence it.Methods Sixty boys aged 4-12 years with hemophilia were recruited.Their ADL ability was assessed using the functional independence score in hemophilia (FISH).Results The average FISH score of the sixty patients was 25.75 ± 5.71.The percentage of the patients who had experienced functional declines in self-care,transfer and locomotion abilities were 46.7％,55％ and 75％,respectively.There was negative correlation between the patients＇ ages,the replacement therapy they had received and their FISH scores.The Spearman correlation coefficients were-0.371 and-0.388 respectively.The duration and frequency of the preventive therapy and appropriate exercise they had experienced were positively correlated with their FISH scores.The Spearman coefficients were 0.339.0.317 and 0.436 respectively.But there was no correlation between FISH scores and the level of coagulating factor in the subjects＇ blood,their frequency of bleeding in the most recent three months,muscle bleeding,the dosage of replacement therapy or the patient＇s place of residence.Conclusion Seventy-five percent of young,male hemophilia patients manifest a decline in ADL ability.Declines in locomotion occurred the most frequently.The ADL ability of young hemophilia patients probably declines with age.Long term preventive therapy and appropriate exercise would be helpful to maintain ADL ability.

Objective To analyze the sonographic characteristics of endometrial lesions to increase the diagnostic accuracy of the sonography. Methods 69 patients diagnosed as endometrial lesions by transvaginal color Doppler sonography(TVCDS) were retrospectively analyzed. The findings of TVGDS were compared with pathological diagnosis. Results The total diagnostic accordance rate of TVCDS was 91. 3% for endometrial lesions, including 93. 0% for endometrial polyps,91. 3% for endometrial hyperplasia,84. 2% for endometrial cancer, and 95. 0% for submucosal uterine fibroids respectively. In addition,the blood flow image rate and arterial resistance index(RI) had significant difference (P＜0.05) between endometrial cancer and other benign lesions of endometrial, and all of these have obvious correlation with pathological findings. Conclusion TVCDS was an effective screening method for the diagnosis and differential diagnosis of endometrial lesions.

Objective To construct a recombinant plasmid pLEGFP-mIFN? for developing the genetical therapy of tumor by stem cells.Methods PCR was used to amplify the mIFN? gene from plasmid pcDNA3.1-mIFN? in which the mIFN? was cloned.A new plasmid,pLEGFP-mIFN?,was then constructed by inserting the amplified mIFN? gene into pLEGFP-C1.Restriction analysis and sequencing were used to confirm the structure of pLEGFP-mIFN?.Neural stem cells were transfected by supernatant of PA317.Results A DNA fragment in size of 500 bp was amplified.Restriction analysis showed that the amplified gene was inserted into pLEGFP-C1 correctly.(Conclusion The) plasmid pLEGFP-mIFN? is constructed successfully,and it can transfect neural stem cells.

ObjectiveTo investigate the diagnosis value of intracardiac echocardiography in cesarean section scar pregnancy. MethodsCavity ultrasound was applied in patients of cesarean scar pregnancy with sonographic features and treatment. ResultsIn 52 patients, ultrasound diagnosed accurately in 45 cases, diagnosis accurate rate was 86.5％ (45/52). Sonogram showed gestational sac simply type in 16 cases, mixed echo-type( or mass-based) of 36cases. In 52 cases of CSP, according to their clinical and sonographic features ,2 cases of uterine scar lined laparotomy and the uterus excision repair;three cases of laparoscopic removal of uterine scar pregnancy lesion repair. 6 patients to methotrexate;five cases of abdominal surgery;36 routine uterine arterial embolization in the ultrasound monitor after curettage. ConclusionUltrasound could apply in cesarean scar pregnancy timely,and accurate diagnosis was made;sonographic features according to the patients,help choose the appropriate clinical treatment,and assess efficacy.

Objective To explore the value of real‐time three‐dimensional speckle tracking imaging (RT‐3D‐STI) in the early detection of globle and regional left ventricular myocardial systolic function in patients of coronary stenosis without regional wall motion abnormality. Methods 64 coronary stenosis patients without regional wall motion abnormality were divided into 2 groups according to angiography (CAG):without coronary stenosis group( n = 20) and coronary stenosis group ( n = 44), and all the myocardial segments of the patients were divided into 5 groups:without coronary stenosis group normal myocardial segments ( n = 340 ), coronary stenosis group normal myocardial segments ( n = 235 ), mild coronary stenosis myocardial segments( n = 126), moderate coronary stenosis myocardial segments( n =213) and severe coronary stenosis myocardial segments( n =174). Real‐time three‐dimensional full volume of left ventricle was obtained, left ventricular global area strain (GAS)and regional area strain(AS) of 17 myocardial segments, etc, were measured by RT‐3D‐STI respectively. The parameters between the two groups and among the five sub groups were compared, the correlation between GAS and coronary artery Gensini score was also analyzed. Results GAS in coronary stenosis group significantly reduced compared with without coronary stenosis group( P <0 0.5) S.egmental AS gradually reduced with the increasing of the degree of the coronary stenosis. There was no statistically difference of segmental AS among without coronary artery group normal myocardial segments, coronary stenosis group normal myocardial segments and mild coronary stenosis myocardial segments( P >0 0.5) S.egmental AS in moderate and severe coronary stenosis myocardial segments significantly reduced ( P < 0.05 ), and that in severe coronary stenosis myocardial segments was lower than that in moderate coronary stenosis myocardial segments, the differences were statistically significant ( P < 0.05 ). In addition, GAS was significantly correlated with coronary artery Gensini score( r =0.828, P <0.01). Conclusions RT‐3D‐STI can quantitatively assess the early changes of left ventricular globle and regional myocardial systolic function in patients with coronary stenosis.

ve To study the growth curve of Alexandrium tamarense and the toxicity of paralytic shelfish poisoning(PSP) selected in different growth phases, as well as to quantitatively analyze the toxins and compare the different efficiency of breaking cell wall and their virtues and defects between freezing-thawing method and supersonic method, and to provide theoretical basis for the selection of the methods for extraction of PSP. Methods Cells were collected by suction filter, cell wall was broken by freezing-thawing method and supersonic method, the toxicities of Alexandrium tamarense among different growth phases were detected and compared. Results The growth of Alexandrium tamarense typically showed three phases which included a lag phase(0~9 days), an exponential growth phase(10~18 days), and a stationary phase(19~22 days) . The maximum toxicity of cells in the stationary phase appeared, especially in day 21 when the population of cells reached to 9.43?10-6 MU/cell, although the cells in the exponential phase grew fastest. Toxicity of the cells in the lag phase broken by freezing-thawing method was more stronger than that by supersonic method, that meant the toxicity of cells was lowered after the supersonic treatment. In additional, the filtering method for collecting cells presented a lower recovery rate of average 69.0% . Conclusion As for the comparing of freezing-thawing method and supersonic method, it was found that, when cells were in the same phase, the supersonic method had remarkable advantages, but it also had the disadvantage of lowering the tox-icity of cells. When in the same condition of growth and method of fragmentation, cells in the lag phase were broken most easily, and cells in the stationary phase were more difficult to be broken than those in the other two phases. Toxicity of cells was larger and larger with the time of incubation. The toxicity of cells in the lag phase had reached a higher intensity, although toxicities of cells in the next two phases would increase a little.

Objective To investigate the efficacy of active immunization combined with dydrogesterone in the treatment of recurrent spontaneous abortion,and the expression of serum leptin(LP)and adiponectin(ADPN).Methods 51 cases of inpatient and outpatient treatment of repeated abortion of 51 pregnant women from January 2014 to December 2015 in Ningbo Fenghua People's Hospital of Zhejiang Province were selected,and 50 cases of normal pregnant women and normal nonpregnant women 50 cases as a control object.Depending on the treatment method,patients with recurrent spontaneous abortion were divided into combination group(n=26)and monotherapy group(n=25).The levels of LP,ADPN and β-HCG were measured before and after treatment,and the number of successful pregnancies.Results The levels of LP and ADPN in serum were measured in three groups of women,there were statistically significant differences between the two groups(P<0.05).The serum levels of β-HCG in the pregnant women with recurrent spontaneous abortion group were(3914.5±2548.2)mIU,and the normal pregnancy group was(7124.5±2847.6)mIU,the difference was statistically significant(P<0.05).After treatment,the levels of LP,ADPN and β-HCG in the combination group were significantly increased,and successful pregnancy as high as 88.46％,better than the level of monotherapy group,the difference was statistically significant(P<0.05).Conclusion Serum LP,ADPN lower levels of pregnant women prone to recurrent spontaneous abortion,through active immunization combined with dexamethasone treatment,can significantly improve the hormone level,improve the success rate of pregnancy.

Objective: To investigate the effect of function of knee on quality of life (QOL) in patients with knee osteoarthritis (OA). Method: Forty patients with knee OA were recruited. Their function, pain and range of motion (ROM) of knee were assessed with Lequesne index, Lysholm knee score (LKSS), visual analogue scale (VAS) and protractor measure respectively. QOL was evaluated with arthritis impact measurement scale (AIMS). Result: The scores of AIMS, Lequesne and VAS in female were significantly higher than that in male. And the LKSS in female was significantly lower than that in male (P<0.05). There was a positive correlation between AIMS score and Lequesne index, VAS score and limit of ROM, but a negative correlation between AIMS and LKSS (P<0.05). Conclusion: The function of knee and QOL in female patients with OA were worse than that in male patients. Pain, limited ROM and weaken function in knee could effect QOL in OA patients to some extent. Health education and clinical treatment should be provided to improve the function of knee and QOL in OA patients.

Objective:This study was aimed to investigate the influence of CDK7 siRNA on the sensitivity of endometrial carci-noma cell line HEC-1-A to cisplatin (DDP)-based chemotherapy. Methods:Different CDK7 siRNA fragments were synthesized based on the designs of the CDK gene sequence and were transfected into HEC-1-A. Real time reverse transcription polymerase chain reac-tion (RT-PCR) and Western blot analysis were employed to demonstrate the effects of transfection. The best CDK7 siRNA was chosen to specifically silence CDK7 expression in HEC-1-A.The sensitivity of the cells to DDP therapy before and after transfection was deter-mined by methyl thiazol tetrazolium (MTT) cytotoxicity assay, flow cytometry, and Hoechst/PI double-staining fluorescence microsco-py. Results:A total of four different CDK7 siRNA segments were designed and successfully transfected into HEC-1-A cells. The inter-ference effect in each group was confirmed by real time RT-PCR and Western blot assays. CDK7-423 was determined as the best per-forming CDK7 siRNA (over 70%) to transfect into HEC-1-A cells. MTT cytotoxicity test showed that IC50 of DDP decreased to a range from 45.122 μg/mL and 3.200 μg/mL after inhibition of CDK7 expression. DDP toxicity to the endometrial carcinoma cells sig-nificantly increased (P<0.05). Flow cytometry revealed that the average cell apoptosis rate significantly increased after the inhibition of CDK7 expression (11.66%to 37.57%, P<0.05). Similar results were observed using Hoechst/PI double-staining fluorescence microsco-py, and the number of apoptotic corpuscle demonstrated an apparent increase in the low CDK7-expressing group compared with the pa-rental cells. Conclusion:After the downregulation of CDK7 expression by CDK7 siRNA transfection, DDP chemotherapy sensitivity and apoptosis of endometrial carcinoma cells significantly increased. Further research is anticipated on the use of CDK7 as a new treat-ment target for endometrial carcinoma.

Objective To evaluate the efficacy and safety of mosapride in the treatment of gastroesophageal reflux disease (GERD). Methods Papers of PubMed,EMBASE,OVID,VIP Database and WANGFANG Data were searched and reviewed according to international Cochrane Collaboration.Results A total of 21 trials (1666 patients) were included.The treatment group was with mosapride and PPI,the control group was with single PPI or PPI and placebo.The Results of Meta analysis showed:for non-erosive negative reflux disease,there was no significant difference in efficacy between two groups.As for erosive esophagitis treated with mosapride (5mg):There was no significant difference in the 4-week healing rates between treatment group and control group (P =0.06).The 4-week symptom relief rate of treatment group was better than that of control group (P＜0.01).The 6-week healing rate of treatment group was better than that of control group (P＜0.01).The 6-week symptom relief rate of treatment group was better than that of control group (P＜0.01).There was no significant difference in the 8-week healing rate of esophagitis between treatment group and control group (P =0.05).The 8-week symptom relief rate of treatment group was better than that of control group (P＜0.01).For erosive esophagitis treated with mosapride 10mg,.The 8-week symptom relief rate of treatment group was better than that of control group (P=0.02).Conclusion According to the meta-analysis of these 21 trails,mosapride was safe and effective in the treatment of GERD.However due to the quality of the literatures,the above findings should be treated with caution.