Objective: To verify whether there exists melatoni n(Mel) receptor in human embryonic nervous system. Methods: Spec ific binding of Mel to embryonic brain and spinal cord was measured by radioliga nd binding assay. Results: 125　I-Mel binding s ites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS d ose-de pendently inhibited the binding. Conclusion: The results demonst rate the presence of specific binding of Mel in human embryonic brain and spinal cord. GTPγS has some effect on 125　I-Mel specific binding,support ing the theory that Mel receptor is coupled to inhibitory G-proteins.

Objective: To discuss the purposes and methods of determining and employing operational intervention levels in preparedness and response for a nuclear emergency. Methods:According to the safety standards and technical reports issued by the International Atomic Energy Agency (IAEA) and combined with specific conditions in China, the operational intervention levels suitable for applying in China are analyzed. Results: The newly published IAEA technical report provided special operational intervention levels for emergency at a light water reactor. The recommended values are practical that our country can use for reference. Conclusion: It is suggested that the related branches for response to nuclear emergency in China establish feasible operational intervention levels by referring to the IAEA reports and taking into account the special conditions of nuclear facilities in China, in order to improve the capability of medical response to nuclear emergency.

Cell Adhesion Molecules ; metabolism ; Cells, Cultured ; Endothelial Cells ; cytology ; metabolism ; physiology ; Humans ; Integrins ; metabolism ; Membrane Proteins ; metabolism ; Neoplasms ; metabolism ; pathology ; Organ Specificity ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Saphenous Vein ; cytology ; Tumor Cells, Cultured ; Umbilical Veins ; cytology

Pain just tolerable was caused intermittently by electric stimulation on rat's tail or by distented baloon in stomach for 30 min. The changes of intermediate lobe are summarized as follows: (1) The cell body, nucleus or nucleolus was hypertrophied. The number of rough endoplasmic reticulum, particularly of its vesicular cisternae was increased. Golgi complex enlarged. The ribosome multiplied. All of these change indicated that the activity of cells was increased and the rate of synthesis enhanced. (2) Both the secretory granules shifted to the periphery of cell body and some of them showed pale density. These changes were interpreted as increase of seretory activity. (3) The mitochondria became enlarged and the crsitae in them were changed into tubular form. This suggested an acceleration of cell metabolism, which might provide a large amount of energy. (4) There was no degenerative change. (5) The cytological changes caused by visceral pain were simillar with, but more striking than, those caused by somatic pain. (6) The secretions were increased, among which ?-endorphin and ACTH etc were known to be closely related to analgesia.

Our experiments were designed to observe the cytological changes of the anterior lobes in rats pituitary at electron microscopic level after electric pain-stimulation. obvious changes were founded in the ACTH, GH and PRL cells and slight changes in the FSH and TSH cells. However, the LH cells remained to resemble the normal cells. In the experiment group of the somatic painful stimulation the results were as follows: The ACTH cells were irregularly shaped, with extended thin and long plasmatic processes which interdigitated with GH cells. The processes filled with secretory granules. Some of the Secretory granules aligning along the plasmatic membrane showed pale density. The flat cisternae of rough endoplasmic reticulum (RER) appeared to be slightly dilated. The sizes of the bodies of GH and PRL cells increased. In the GH cells there were numera media cisternae in RER, their Golgi complex was well developed, and the secretory granules were distributed to the periphery of the cell bodies. All these phenomenous showed the secretory activity of these cells was enhanced. The PRL cells contained well developed Golgi complex and abundant RER. In the experiment group of the visceral painful stimulation the results were as follows: There were more striking changes of parenchymal cells of anterior lobes in rats pituitary than those of the experimental group of the somatic painful stimulation. The cell bodies of ACTH, GH and PRL cells showed hypertrophy, the nucleus enlarged and nucleolus were relatively prominent. The dense granules decreased in number and the pale or empty visicles almost situated near the plasmic membrane in ACTH cells. In the GH cells large vacuoles were distributed throughout the cytoplasma. The number and density of the secretory granules decreased. In PRL cells the Golgi complex were extended, the dictyosomes increased, in addition, RER, polyribosomes and mitochandria were more aboundant than that in these normal cells. These cytological changes suggested secretory and synthetic activity of these cells were enhanced. At last, pain in relation to several hormones such as ?-endorphin, ?-LPH, ACTH and ?-MSH etc. were discussed by the author.

The purpose of this research work is to study the influence of experimentally produced pain on the uhrastructural changes of the nerve endings of the neurosecretory fibers from hypothalamus to neurohypophysis.Twenty adult male rats, weighing 200～300 g were divided into 3 groups: (1) Six animals were stimulated through two electrodes fixed on the tail to produce somatic pain. (2) Six animals were stimulated by increasing pressure in the stomach through an air balloon to cause visceral pain. And 8 animals served as control.All the animals were sacrificed and their neurohypophysis were fixed in 40％ glutaraldehyde and 1％ OsO_4 successively. Sections were cut with an LKB microtome and observed under H500 electron microscope.The following conclusions were drawn from the observed results:1. The neurosecretory granules in the fibers from hypothalamus to neurohypophysis grew up to maturity by increasing in size gradually as they passing down from hypothalamus to neurohypophysis.2. Basing on the morphological exhibition of the neurosecretory granules, we get the impression that the neurosecretion might be released either by molecular dispersion or by exocytosis.3. There were a great number of discrete smooth vesicles grouped in cluster in the dilated parts of neurosecretory fibers. These vesicles had been called as "synaptic" or "synaptoid" vesicles. They might be really synaptic vesicles and containing acetylcholine. Other vesicles with irregular profile and varying in size, collapsing vesicles and vacuoles might be the residues of the neurosecretory granules after discharging their content.4. Many synaptic vesicles were concentrated at the axolemma of the dilated parts of neurosecretory fibers and the membranes were thickened and leaving a synaptic cleft.5. In the exprimental groups after pain stimulation the neurosecretory granules decreased in number and the synaptoid vesicles increased in number and the neurosecretory granules showed a wide pale halo. The number of irregular vesicles and ghost of granules were increased. This phenomenon might show that the excretion of neurosecretory material and neurotransmitters was enhanced.6. Neurohemal zones were the barrier for the neurosecretory material entering into blood. Acetylcholine, endogenous opiatelike substances and gamma-aminobutyric acid (GABA) might play a control role for neurosecretion at this site.7. This experimental results supported the action mechanism of endogenous opaitelike substance and morphine could produce an important role directly in neurohypophysis.

Objective To investigate the situation and change of antifungal resistance in clinical Candida and other fungal iso- lates from 5 hospitals in diverse geographic region of China.Methods Antimicrobial susceptibility testing of 8 000 fungat iso- lates collected during 2001 and 2005 were carried out with 25?g fluconazole disk and 1?g voriconazole disk using disk diffusion method as recommend by CLSI/NCCLS M44-A.Disk test plates were automatically read and results were recoded with the BIOMIC Image Analysis System.The equivalent MICs were automatically calculated by the BIOMIC System software.Results The proportion of Candida atbicans and non-Candida albicans (e.g.Candida glabrata) in the total fungal isolates did not change significantly from 2001 to 2005.The susceptibility rate of C.albicans to fluconazole and vorieonazole were stable during 2001 and 2005.However, the resistance to fluconazole and voriconazole increased variably in C.glabrata and other non-Can- dida albicans fungal isolates during the same period.Conclusions The voriconazole demonstrated higher activity against all yeast species in comparison with fluconazole.The increasing resistance to fluconazole and voriconazole in non C.albicans fungal isolates including C.glabruta suggests the importance of surveillance of fungal resistance in Candida isolates.

Decreasing trend has occurred in incidence of colorectal cancer in developed countries. A shift from left to right in location of colorectal cancer has been recognized,which may be associated with colonoscopy screening,aging population,diet structure modification,increased incidence of cancer and diabe-tes. Factors including sex,race and education may also play a role to some extent. Right-sided intestinal cancers have higher proportion of poor differentiation,terminal stage and mucinous component. Complications and second primary intestinal cancer are more common in right-sided intestinal cancers. Left-sided intestinal cancers tend to be well differentiated and at relatively early stage at diagnosis. With respect to molecular mecha-nism,right-sided cancers are associated with mismatch repair system,while left-sided cancers are related to p53 mutation. Based on the differences in clinicopathology and genetics,it′s implied that left-sided and right-sided intestinal cancers may belong to two different kind of disease. It′s suggested that attentions should be paid differently according to their respective characteristics in clinical practice and trials.

Objective To observe the effects of the compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion on hemodynamic and balance of Th1/Th2 cells of Qi deficiency and blood stasis rat model. Methods Qi deficiency and blood stasis rats were caused by restricted diet, forced swimming and norepinephrine subcutaneous injecting, and treated by compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion of 5∶1, 3∶1, 1∶1 and 1∶2 for 21 days. The indexes of hemorheology were detected with hemorheological analyser, and the level of IFN-γand IL-4 in serum were tested by ELISA. Results Compared with model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 1∶1, 3∶1 and 5∶1 groups reduced the low shear blood viscosity. The spleen index of model group decreased, and compatibility of Radix Astragali and Radix Angelicae Sinensis in 5∶1 proportion group increased spleen index. The level of IL-4 increased and IFN-γ decreased in the serum of model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 3∶1 group increased the level of IFN-γ. Astragalus angelica 5∶1 group decreased the level of IL-4 and increased the level of IFN-γ. Conclusion The compatibility of Radix Astragali and Radix Angelicae Sinensis can improve hemorheology, adjust the balance of Th1/Th2 cells of Qi deficiency and blood stasis rats. The effects were better when Radix Astragali’s dosage greater than that of Radix Angelicae Sinensis, and the group of 5∶1 proportion was the best.

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