70 years old)(P

Acupuncture Therapy ; Aged ; Combined Modality Therapy ; Female ; Humans ; Male ; Middle Aged ; Parkinson Disease ; drug therapy ; therapy

Objective To improve the poor water-solubility of nimodipine,nimodipine-loaded PLEG copoly- met nanopartieles were prepared to avoid the sedimentation of its crystal from water,which could also shun the blood vessel stimulation caused by ethanol,a cosolvent,in the injection.Methods Solvent evaporation method was used to prepare water-dispersing nimodipine-loaded nanoparticles,whose lyophilized powder was further prepared,with PLEG2000 as drug carrier.Particle size,Zeta potential,embedding ratio,drug loading,shape and redispersion ability of the lyophilized powder were determined.Results Concerning the lyophilized nanoparticle powders,the particle sizes were 136nm,the Zeta potentials were -29.90mV,the embedding ratio was 67.9% and the drug loading was 6.17%.Conclusion Prepared nimodipine-loaded nanoparticles solved the problem of poor water-solubility of ni- modipine basically.It also avoided the recrystallization process of the drug and reduced the blood vessel stimulation caused by ethanol in the injection.

Objective　To observe the therapeutic effect of combination of penetrating keratoplasty, cataract extraction and intraocular lenses implantation.Methods　Penetrating keratoplasty was performed simultaneously with cataract extraction and intraocular lenses implantation on 134 cases. Results　One hundred and twenty cases were recorded after following up for 0.5～12 years. Ninety-nine cases (82.5%)of the corneas remained clear. Twenty-one cases (17.5%)of the corneas failed.Ninety-two cases (76.7%)achieved a better visual acuity postoperatively.Eighty-nine eyes(74.2%)were not blind any longer. Conclusion　The combination of penetrating keratoplasty, cataract extraction and intraocular lenses implantation offers advantages of rapid and better rehabilitation of visual acuity with low complication rate.

Objective To explore the change of growth hormone binding protein (GHBP) in children with idiopathic short stature(ISS).Methods Thirty children with ISS from the pediatric department in the Third Hospital of Hebei Medical University and 30 age-and sex-matched normal-stature children were selected from Sep.2006 to Jun.2008.The concentration of GHBP was measured by enzyme linked immunosordent assay.Results Serum GHBP levels in ISS subjects was (3 261.0?646.3) ?g/L,while the serum GHBP levels in normal control group was (2 026.0?497.2) ?g/L,and there was statistically significant difference between both groups(t=-20.67 P

Permeabilized cells containing trehalose synthase from Meiothermus sp.CBS-01 was immobilized by the integration of entrapment in alginate beads with cross-linking and electrostatic self-assembly coating technique.Coating on alginate beads with diazo-resin and poly(styrene sulfonate) can protect the beads from degradation in phosphate buffer,whereas cross-linking with carbodiimide improves the thermostability of trehalose synthase entrapped in alginate beads.By co-immobilization of permeabilized cells using entrapment-cross-linking- coating method,the enzyme recovery was 32%,and the optimum temperature of the enzyme was still 60℃,but the optimum pH was shifted from 6.5 to 7.In batch manner,a maximum conversion yield of 60% trehalose from maltose could be reached by the immobilized cells.Within 4 times repeated use of the immobilized cells,and each time was operated at 50℃ for 24h,more than 50% conversion yield of trehalose could be maintained with less than 20% loss of the enzyme activity.

0.05).(3)The average CTDIvol values were 60?5 mGy,88?10 mGy for 2C_2 and NC_2(C_2)groups,respectively.The corresponding ED values were(12.3?1.0)and(18.0?2.0)mSv,respectively.The CTDIvol and ED values for 2C_2 group were about 32% lower than those of NC_2 group and were statistically significant with P

0.05).Conclusion Three Hybridoma cell lines which secrete the target antibodies with satisfied affinities and specificities have been successfully raised,which provides a basis to produce a domestic-made HCMVpp65 antigen diagnosis kit.

Objective To investigate the event-based prospective memory(EBPM)and time-based prospective memory(TBPM)in schizophrenia patients with concomitant metabolism syndrome,and to provide theory basis for early intervention and treatment.Methods According to inclusion standard,50 schizophrenia patients with concomitant metabolism syndrome and 50 schizophrenia patients without metabolism syndrome matched in age,gender and education were assessed with a neuropsychological battery of tests including EB-PM and TBPM tasks.Results In the EBPM task,there was significant difference between schizophrenia pa-tients with and without metabolism syndrome (3.46±0.91 vs 3.86±0.81, t=-2.326, P=0.022).A statistically significant difference in TBPM was observed between schizophrenia patients with and without metabolism syn-drome (3.02±1.12 vs 3.78±0.89, t=-3.770, P=0.000).Conclusion The schizophrenia patients with me-tabolism syndrome have severer prospective memory impairment than those without metabolism syndrome.

Fluorescence ; Host-Pathogen Interactions ; Luminescent Measurements ; methods ; Luminescent Proteins ; chemistry ; genetics ; metabolism ; Plant Diseases ; virology ; Plant Viruses ; chemistry ; genetics ; metabolism ; Plants ; chemistry ; genetics ; metabolism ; virology