Objective:To investigate the difference of biological characteristics and responses to PDGF-BB between human ovarian carcinoma-derived microvascular endothelial cells and human umbilical vein endothelial cells.Methods:The ovarian carcinoma-derived microvascular endothelial cells were isolated and purifed from ovarian carcinoma tissues.Compared to the human umbilical vein endothelial cells,the biological characteristics and responses to PDGF-BB were observed in OdMEC for the changes of growth,PCNA and VEGF expression.Results:20ng/ml PDGF-BB could enhance both kinds of endothelial cells to proliferate,express PCNA and VEGF.The proliferation and PCNA protein expression in HUVEC were augmented more significantly than those in OdMEC,the protein and mRNA of VEGF expression were augmented more significantly than those in HUVEC.Conclusion:The responses of OdMEC to PDGF-BB were significantly different from those of HUVEC,which provides experiment basis for research on ovarian carcinoma vascularization and antiangiogenesis therapy.

Under the environment of network,the service mode of subject librarian becomes an important strategy of intelligence serves in university libraries.To provide reference for other librarians,this paper summarizes services provided for the academic researchers by using Web of Knowledge platform resources.

Objective To investigate the relationship between renal function and lipid metabolism in patients with chronic renal disease .Methods A total of 76 patients with chronic renal disease were enrolled and classified into chronic renal failure(CRF)group (29 cases) ,chronic nephritis(CN)group(21 cases) ,diabetic nephropathy (DN) group(14 cases) and nephrotic syndrome (NS) group (12 cases) ,and 65 healthy subjects were enrolled in control group ,the relationships between renal function ,lipid metabolism , hypersensitive C-reactive protein (hs-CRP) and 24 hours urinary albumin excretion rate (UAER) were analyzed .Results Urea ni-trogen(Urea) ,serum Cystatin C(CysC) ,cretinine(Cr) ,uric acid(UA) ,β2-microglobulin(β2-MG) ,hs-CRP ,low density lipoprotein (LDL-c) .Lp(a) .24h UAER were significantly higher in CN group;Urea ,Cr ,β2-MG ,CysC ,hs-CRP ,ApoA ,lipoprotein A[Lp(a)] , 24 h UAER were significant higher in DN group;Urea ,hs-CRP ,TC ,TG ,HDL-c ,LDL-c ,ApoB ,Lp(a) ,24h UAER were significant-ly highly in NS group;Urea ,Cr ,UA ,β2-MG ,CysC ,hs-CRP ,TG ,LDL-c ,Apolipoprotein b(ApoB) ,Lp(a) ,24 h UAER were signifi-cantly higher in CRF group .Conclusion With the changes of renal function in patients with chronic renal diease ,the lipid metabo-lism and hs-CRP also change .

ATP-tumor chemosensitivity assay(ATP-TCA)is a tumor chemosensitivity assay in vitro.Beacause of the special merites of A11P-TCA,it is seemed to be the most promising and effective method of tumor chemosensitivity in vitro.Application of ATP-TCA in clinic has got significant effects.ATP-TCA is a good method for individualized chemotherapy.

Objective To study the effect of binocular vision function training for fusion in the eye position and three levels of eye function in adult patients after concomitant strabismus surgery. Methods 56 cases with postoperative concomitant strabismus were randomly divided into training group and control group . In the training group, patients were trained with software to enhance the fusion. After training for 7, 30, 180 and 365 days, the eye position, the fusion and the stereoesthesia were observed and analyzed. Results After training for 365 days, the deteriorative ratio in the training group is 3%, which is significantly lower than control group (23%)(P<0.05). The correction ratio in eye position is 20%, which is significantly higher than control group (4%)(P<0.05). The recovery ratio in binocular vision function is 53%, which is significantly higher than control group (15%)(P<0.05). The recovery ratio in stereaeuity is 46%, which is significantly higher than control group (11%)(P<0.05). Conclusions Fusion training can enhance the recovery of fusion and stereaeuity, decrease the deteriorative ratio, and correct the eye position after surgery in concomitant strabismus patients.

Objective To prepare mouse anti-human monoclonal antibodies against B7-H6 and to identify their biological characteristics. Methods The B7-H6 gene was cloned by RT-PCR from a human lung adenocarcinoma cell line ( A549 ) and then subcloned into the eukaryote expression vector pCMV3 to construct the recombinant vector pCMV3-B7-H6. The recombinant vector pCMV3-B7-H6 that was verified with enzyme digestion and gene sequencing was transfected into NIH/3T3 cells by electroporation. BALB/c mice were immunized with the successfully transfected cells named 2H8 through intraperitoneal injection. The monoclonal antibodies against human B7-H6 with the advantages of high affinity and specificity were pre-pared by using hybridoma technology. Western blot assay and flow cytometry analysis were used to identify the specificity of prepared monoclonal antibodies. Results The recombinant eukaryotic expression vector encoding B7-H6 was successfully constructed. Two hybridoma clones that stably secreted monoclonal anti-bodies against B7-H6 were screened out by using flow cytometry analysis and the monoclonal antibodies se-creted by them were belonged to IgG2a isotype. Specific reactions between B7-H6 and the secreted mono-clonal antibodies were confirmed by Western blot assay and flow cytometry analysis. Conclusion The mon-oclonal antibodies which recognized B7-H6 specifically were prepared successfully.

Objective To investigate the effects of Astragalus membranaceus and Angelica sinensis on the hemodynamics and pathological features of arterial wall in restenosis of blood vessel in rats in order to find the potential mechanism of the effect of Astragalus membranaceus and Angelica sinensis in preventing restenosis after ablation of intima. Method Thirty SD rats were randomly divided into 5 groups (6 each):control group (C),model group (M),Astragalus membranaceus treatment group (H),Angelica sinensis treatment group (D),and combined Astragalus membranaceus with Angelica sinensis treatment group (HD). The restenosis model was reproduced by denuding rat's aorta endothelium with balloon catheter in all the groups except group C. After intramuscular injection of Astragalus membranaceus 0.42ml/(kg?d) in group H,Angelica sinensis 0.21ml/(kg?d) in group D,and Astragalus membranaceus 0.42ml/(kg?d) together with Angelica sinensis 0.21ml/(kg?d) in group HD for 21 days,the changes in hemodynamics and the pathological features of arterial wall were observed in all the groups. Result Compared with rats of group C,in which the intima was smooth and the smooth muscle cells (VSMCs) in media were orderly arranged,the intimal thickness of aorta,pulsatile index (PI) and resistance index (RI) increased,while the diameter of lumen,blood velocity decreased remarkably in group M. Compared with the rats in group M,the extent of hyperplasia and thickness was notably reduced,RI and PI were lower while blood velocity was lowered in group H,D and HD after being treated for 21 days. Conclusion Hyperplasia induced by remaval of endothelial cells may be reduced and blood circulation may be improved to different extents by individually or simultaneously use of Astragalus membranaceus and Angelica sinensis,thus preventing restenosis of the aorta after removal of its intima.

Blood Glucose ; analysis ; Diabetes Mellitus ; epidemiology ; prevention & control ; Humans ; Life Style

Objective To study the efficacy of complete hemihepatic vascular exclusion (CHVE) in the prevention of tumour dissemination during liver resection for hepatocellular carcinoma (HCC).MethodsA case-control study was carried out on 281 patients who underwent liver resection for HCC between February 2006 and April 2010.89 patients underwent hepatic resection using CHVE (group A) and 192 using Pringle maneuver (group B).The postoperative 1-,2-,3 year rates of intrahepatic recurrence and lung metastasis,and the overall disease-free survivals were compared between the two groups.ResultsThe incidence of lung metastasis at 1 year after operation was significantly lower in group A than group B (6.7％ vs.15.1％,P=0.048).There were no signilicant differences in the incidences of lung metastasis at postoperative year 2 and 3 between the two groups (15.7％ vs.25.0％ and 32.6％ vs.30.7％ ; P＞0.05).The differences in the postoperative 1-,2-,3- year intrahepatic recurrence rates were not significant between the two groups (20.2％ vs.31.8％,51.7％ vs.60.3％,68.5％ vs.79.2％,respectively; P＞0.05).The median survivals of the two groups were 25.9 months and 21.7 months,respectively.Patients in group A had a significantly higher overall disease-free survival rate than patients in group B (P=0.035).ConclusionsCHVE was efficacious in reducing the incidence of lung metastasis and in improving the overall disease-free survival in patients after liver resection for HCC.CHVE contributed in preventing intraoperative tumour dissemination and in improving prognosis.

Objective To explore the factors influencing health management demand by community residents.Methods Using data from the survey of Health Management Demand by Community Residents in Hangzhou,this paper examined the influence factors with the logistic model from such four aspects as individual characteristics,economic characteristics,health level and health awareness.Results The health awareness poses a positive impact on health management demand;the level of health has a negative impact while income has an inflection point(5 000 yuan per month).However,individual characteristics have no significant effect. Conclusion Such measures as enhancing health management awareness of the residents,building a health management system centering on communities and supported by health management firms,and building health management information platform,can effectively satisfy such demand.