Female ; Fetal Diseases ; etiology ; Hemangioma ; complications ; Humans ; Infant, Newborn ; Splenic Rupture ; etiology ; Thrombocytopenia ; complications

Female ; Hemangioma ; therapy ; Humans ; Infant, Newborn ; Syndrome ; Thrombocytopenia ; therapy

OBJECTIVE: To investigate the effect of vitamin K2 combined with benazepril on human gastric cancer in nude mice. METHODS: SGC-7901 human gastric cancer cells were implanted into BALB/c nude mice to establish the cancer model. Fourteen days after the subcutaneous implantation of cancer cells, 40 nude mice were randomly divided into 4 groups; control group, vitamin K2 therapy group, benazepril therapy group and combination therapy group (vitamin K2 + benazepril). After 14 d, the tumor growth was evaluated, apoptosis indices were examined by TUNEL assay, and VEGF and caspase 3 expression were assessed by immunohisto-chemical staining. RESULTS: Compared to the control group, tumor weights were decreased and apoptosis indexes was significantly increased in the therapy groups (P < 0.05), the protein expression of caspase 3 were up-regulated, and VEGF were decreased in the therapy group (P < 0.05), and all the changes above were more significant in combination therapy group than vitamin K2 or benazepril group (P < 0.05). CONCLUSION: The combination of vitamin K2 and benazepril enhances anti-tumor efficacy possibly through angiogenesis suppression and apoptosis induction. Copyright 2012 by the Chinese Pharmaceutical Association.

To investigate the effects of Tongxinluo capsule on sciatic nerve apoptosis in spontaneous type II diabetic KK/Upj-Ay mice, in order to explore its mechanism for improving diabetic peripheral neuropathy (DPN). KK/Upj-Ay mice were selected as the DPN animal model and randomly divided into the model, Tongxinluo low, middle and high group (1, 2, 4 g x kg(-1)). C57BL/6 mice were selected as the control group. Mice were given intragastrically for 12 weeks. Paw withdrawal latency, motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were detected. Apoptotic rate were detected by FCM. Bcl-2, Bax, Caspase-3 mRNA and protein expression in sciatic nerve were examined by Real-time PCR and Western blot. p38MAPK, p-p38MAPK expression were examined by Western blot. In this study,the authors found that Tongxinluo capsule could increase paw withdrawal latency, MNCV and SNCV. Apoptotic rate of sciatic, the expression of Bax and caspase-3 were lower, while Bcl-2 expression was higher in Tongxinluo group than those in model mice. The expression of p-p38MAPK significantly decreased in Tongxinluo group. The results showed that Tongxinluo capsule has protective effects on diabetic peripheral neuropathy of mice via inhibiting cell apoptosis and suppressing the expression of p-p38MAPK.
Animals ; Apoptosis ; drug effects ; Capsules ; administration & dosage ; Diabetic Neuropathies ; drug therapy ; physiopathology ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Sciatic Nerve ; cytology ; drug effects

Animals ; Disease Models, Animal ; Female ; Fibrosis ; metabolism ; pathology ; Hydroxyproline ; metabolism ; Lung ; drug effects ; metabolism ; pathology ; Mice ; Thrombospondin 1 ; pharmacology

The aim of this study is to elucidate the protective and anti-apoptotic effects of insulin-like growth factor 1 ( IGF-1 ) against β-amyloid (Aβ) and investigate the effect of IGF-1 on Aβ-induced tau phosphorylation. Cell viability was measured using the MTT (3-(4,5-dimethylthiazolyl-2 )-2,5-diphenyltetrazolium bromide) assay, early apoptosis and late apoptosis/necrosis were analyzed by flow cytometry using Annexin V-FITC and propidium iodide (PI) double staining, and morphology was examined by Hoechst 33342 staining. Tau phosphorylation was detected using AT8 immunostaining. Preincubation of cultured rat hippocampal neurons with IGF-1 for 24 h prevented cytotoxicity induced by Aβ25-35 for 48 h. The MTT value significantly increased from 54.51% to 61.8% of the control group, and the percentage of Hoechst 33342-positive cells decreased from 30.77% to 22.81%. Incubation with Aβ25-35 for 48 h caused a marked increase in the percentages of Annexin V-FITC single-labeled cells (Annexin V +/PI-) and Annexin V/PI double-stained cells (Annexin V +/PI + ) (3.41% and 19.47% , respectively), which were significantly decreased by pretreatment with 100 ng/ml of IGF-1 for 24 h (to 2.98% and 15.16% , respectively). Aβ25-35 treatment increased tau phosphorylation and AT8 positive cells were 41.84%. This effect could be inhibited by different concentrations of IGF-1. Our findings showed that IGF-1 protected against Aβ-induced cytotoxicity, decreased the percentage of early and late apoptosis/necrosis cells, and inhibited tau phosphorylation, which may be the cellular mechanisms for its neuroprotective action.

OBJECTIVE:To analyze the rationality of the preventive use of antibiotics in Chinese ophthalmologic patients with type Ⅰ incision.METHODS:By means of retrospective study,a total of 1 273 ophthalmologic cases from 113 hospitals of 2006～2007 from National Monitoring Network of Ministry of Health for Clinical Application of Antibiotic with type Ⅰ incision involving preventive administration of antibiotics via different routes were analyzed.RESULTS:The local administration (eye drops and local injection) was rational on the whole,but the systemic administration (intravenous or oral administration) was irrational to some degree in which the drug grade was on the high side,the antibacterial spectrum was inappropriate,and the medication time was delayed.CONCLUSION:Preoperative local administration and rational use of dosage forms should be emphasized in the preventive use of antibiotics for patients undergoing ophthalmologic surgery.

Objective To establish the quality control method for Youqing Granules. Methods Qualitation discrimination of Rhizoma Cyperi and Raidix Paeoniae in this formula was identified by TLC. Quantitive analysis of Astragaloside A which is the effective element of the formula was adopted by RP-HPLC-ELSD. Result The specks in TLC were clear and easy to discriminate. The precision and reproducibility of the RP-HPLC-ELSD method were fine. In 1.032～5.16 ?g, the content of Astragaloside A had good linear relationship. The average recovery rate of sample was 99.0516 %, and RSD=1.05%. Conclusion The quality standard established on this study can control the quality of the products, the method is viable.

Objective Our study is to observe the effects of conjugated equine estrogen (CEE) combined with medroxyprogesterone acetate (MPA) on hemorheology in postmenopausal women in order to evaluate mechanism responsible for the increase in the incidence of cardiovascular disease after menopause and also to provide proper instruction and advice of hormone replacement therapy (HRT) for postmenopausal women Methods 60 healthy postmenopausal women were assigned to 3 groups randomly, 20 cases in each group Group A: CEE 0 625 mg/d+MPA 2 mg/d+calcium carbonate co. (Ca D) 600 mg/d Group B: CEE 0 3 mg/d+MPA 2 mg/d+Ca D 600 mg/d Group C: Ca D 600 mg/d only served as control Measurements of hemorheological parameters were performed before and 6 months after treatment Results After 6 months of HRT, group A: blood viscosity under the shear rate of 94 5 s -1 dropped from (5 23? 0 37) mPa?s to (5 03? 0 43) mPa?s ( P 0 05) Conclusion Both different dosages of CEE have favor effects on blood viscosity and erythrocyte deformabillity, which could play the role in preventing cardiovascular disease in postmenopausal women

Background Nomal binocular vision is achieved through accommodation and vergence.Anisometropia is associated with abnormal visual development,but its impact on accommodation and vergence is unknown.This study is helpful to make a reaonable correction plan for anisometropia.Objective This clinical study was to examine the binocular vision function in myopic anisometropia and evaluate the influence of myopic anisometropia on binocular vision function.Methods Seventy subjects with myopic anisometropia ≥ 1.0 D were recruited in Tianjin Eye Hospital and Tianjin Vocational Institute from 2006 to 2009.The subjects were assigned to the low anisometropia group with the 1.0 D ≤ spherical equivalent difference ＜ 2.5 D and the moderate and high anisometropia group with spherical equivalent difference ≥2.5D,and other 35 individuals with spherical equivalent difference ＜ 1.0 D were enrolled as the without anisometropia group.Binocular vision function parameters were examined in all the individuals,including comprehensive refractometer to check the diopter,Worth 4 dot to examine the binocular vision function,different distance of vertical fusional amplitudes,accommodative convergence/accommodation (AC/A) and stereoscopic vision.Written informed consent was obtained from each subject prior to the study.Results After anisometropia was completely corrected,the normal rate of Worth 4 dot examination was 94.3％,80.0％ and 60.0％ in the without anisometropia group,low anisometropia group,moderate and high anisometropia group,respectively,showing a statistical difference among groups (x2 =12.137,P＜0.05),and the normal rate of 4 dot was less,and that of 5 dot was higher in the moderate and high anisometropia group compared with the without anisometropia group and low anisometropia group (P＜0.05).No significant difference was found in the normal rate for phoria of distance among the three groups (x2 =4.489,P=0.344).The normal rate for near phoria of distance was 65.7％,42.9％ and 37.1％ in the without anisometropia group,low anisometropia group,moderate and high anisometropia group,respectively,with significant difference among them (x2 =6.045,P＜0.05).The normal rate of stereopsis was 91.4％,77.1％ and 54.3％ in the without anisometropia group,low anisometropia group,moderate and high anisometropia group,respectively,which was statistically different among groups (x2 =12.863,P =0.002),and that in the moderate and high anisometropia group was significant declined in comparison with the without anisometropia group (x2 =12.208,P ＜ 0.05).The difference of distance phoria,AC/A,negative relative accommodation (NRA),positive relative accommodation (PRA),accommodation amplitude and binocular accommodative lag were statistically different among groups (P＞0.05).Conclusions The normal rate of the stereopsis,Worth 4 dot and the phoria of near distance are decreased with the increase of anisometropia,but other ocular motor parameters are normal in anisometropic subject.