One of the human GM-CSF cDNA was successfully isolated with a new screening method. Up 1/170 of positive rate was reached. The COS-7 cells were transfected with the cDNA clone and the resulting supernants contained colony-stimulatory activities. Human GM-CSF cDNA was also subcloned into a expression plasmid pKPL-2D and the unfused recombinant human GM-CSF was expressed in E. coli. The expressed products could maintain in vitro the growth of a cell line BMU- 2 and stimulate its colony formation. In addition, it could increase the colony formation of bone marrow cells from the patients with acute non-lymphatic leukemia (ANLL).

Intestine is one of the fastest cellular renewal tissues and organs in the body cells,and intestinal mucosal epithelial cells keep self-renewal constantly all the life.Its effect relys on stem cells continued proliferation,differentiation and replaces the outer terminal differentiated cells.Under the stimulation after injury,the repairment of stem cells is initiated,the proliferation and differentiation of intestinal stem cells are stimulated throughout molecular mechanisms.Studys found that intestinal stem cells involve in the repairment of the gut during stress,trauma,anoxia,radioactive damage,inflammatory bowel disease and short bowel syndrome.In this paper,we briefly overviewed the definition,location,and differentiated regulation stem cells,and influence factors in the intestinal repairment.

Objective To explore the impact of curcumin on antistreptolysin“O”(ASO)and interleukin 8 (IL-8)level changes in serum of psoriatic mice model induced by phorbol ester(TPA).Methods 132 SCID mice were selected and divided into three groups.43 mice in normal group, 46 mice in model group,and 43 mice in intervention group.The mice of model group and intervention group were set up psoriasis model induced by TPA. Mice of the three groups were bred in the same environment,curcumin was injected in the intervention group mice,0.02 g/100 g,once a day,two weeks for a period of treatment.ASO and IL-8 level were compared amorg the three groups of mice after treatment.Results Before the treatment,compared with the normal group,positive rate of ASO in model group and intervention group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,the level of intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05 );before the treatment,compared with the normal group,IL-8 level in intervention group and model group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,level of the intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05).Conclusion Curcumin could effectively reduce ASO and IL-8 level in serum of psoriatic mice model, improve the clinical symptoms of psoriasis mice,it is an effective drug for treatment of psoriasis.

Objective To establish the optimal extraction technology for Yishen Jiangu Granules.Methods The orthogonal test was employed for selecting the optimum of extraction technology. The investigation factors were the consumption of water, the times and time of extraction. The extraction technology of Yishen Jiangu Granules was screened by the transfer rate of icariin in herb epimedii and yield of extraction.Results The best extraction condition was as follows:10 times of water, extracted for 3 times , each time was 3 h,2 h and 2 h. Conclusion This extracting technology for Yishen Jiangu Granule was objective, feasible, stable,reasonable and with good reproducibility.

Objective To establish the optimum preparation process for Yinxingye Dropping Pills. Methods The preparation conditions, such as different kinds of primary substance, cooling agent, the proportion between the extracts and primary substance, etc, were studied with L9 (34) orthogonal design. Results At the following conditions could the best was 30 d/min, dropping distance was 8cm, and cooling temperature was 10℃. Conclusion This preparation process was suitable for both laboratory test and industrial production for Yinxingye Dropping Pills.

Objective To compare the speech perception performance of BTE hearing aids with different directional microphones. Methods 20 adults with sensorineural hearing loss participated in the research. Two digital hearing aids were programmed for adaptive directional, fixed directional, and omni-directional microphone patterns. The multi-talker noise was presented in three conditions: 180?,?90?, and 360?. Each subject was tested in nine listening conditions (three microphone patterns and three noise presentations) by using modified Hearing In Noise Test. SNR was obtained in each testing condition. Results Under each noise presentation, the sequence of microphone patterns that obtained SNR from low to high was: adaptive directional, fixed directional, and omni-directional. Conclusion Adaptive directional microphone worked best when the noise source was changing or the noise was coming not only from behind but also from other directions; fixed directional microphone could be employed when the noise came from the rear; omni-directional microphone was suitable for hearing in quiet.

Objective To detect exon deletions/duplications in the DMD gene in Duchenne and Becker muscular dystrophy pedigrees using multiplex ligation-dependent probe amplification method,and explore the usefulness of multiplex ligation-dependent probe amplification analysis as a method for genetic diagnostics in patients with Duchenne and Becker muscular dystrophy.Methods Exon deletions/duplications in the DMD gene were analyzed by multiplex ligation-dependent probe amplification for two pedigrees with Duchenne muscular dystrophy and Becker muscular dystrophy.Patients and carriers were diagnosed by multiplex ligation-dependent probe amplification.Results The pedigree with Duchenne muscular dystrophy was caused by DelEx45-50 mutation,while the pedigree with Becker muscular dystrophy was caused by Dup Ex3-4 mutation.Patients and carriers were diagnosed by multiplex ligation-dependent probe amplification method.Conclusion Exon deletions/duplications in the DMD gene can be indicated by probe copies using multiplex ligation-dependent probe amplification method under standard operating procedure.Multiplex ligation-dependent probe amplification should be considered as a rapid and accurate clinical method for an initial mutation analysis of DMD gene with exon deletions/duplications.

For a valid control of the finance management, the management system is developed again aiming at finance related control links and key control points. In this way, the control is rigorous and integral with simple procedures for check and examination. The key control point is set oriented to note management. Real-time operation is controlled. Post output processing is controlled. With business income and business expenditure as the main line, the control process of the related links are built up. Computer maintenance system is controlled.

Objective: To explore the defense mechanism of patients with Obsessive-Compulsive Disorder(OCD)and to measure the changes in defense mechanisms after the treatment. Methods: Before and after 10- week psychotherapy , 26 patients with OCD were assessed with the Defense Style Questionnaire(DSQ)and Yale-Brown Obsessive-Compulsive Scale(Y-BOCS); DSQ were also used to test 26 normal subjects. Results: (1)The scores of immature mechanism factor (4.5?0.8)were significantly higher in OCD patients than in normal(3.0?0.5),the scores of mature mechanism factor(5.0?0.8)were significantly lower in OCD patients than in normal (5.7?0.7); (2)After 10- week psychotherapy the scores of immature mechanism factor(4.0?0.9)were significantly lower than before (4.5?1.0),the scores of mature mechanism factor(5.7?0.7)were significantly higher than before (5.0?0.9).Conclusion: OCD patients often use immature mechanism, but after psychotherapy, they can use more mature mechanism.

Objective To summarize the experience of nursing cooperation in endoscopic sinus surgery for low temperature plasma radiofrequency ablation of skull base tumor, and to explore the safety and feasibility of this technique. Methods A total of 69 patients with skull base tumor were treated with low temperature plasma radio frequency technology to remove skull base tumor. Results Totally 62 cases of skull base tumors completed total resection in low temperature plasma radiofrequency ablation, 7 cases gained subtotal resection. The operation demonstrated superior advantage of less bleeding, short operation time, less postoperative pain and no adverse events. Conclusions The skull base tumor resection for patients with skull base tumors with low-temperature plasma radiofrequency possesses the advantages of short operation time, less bleeding and thermal damage to the surrounding tissues, besides it can reduce the postoperative discomfort, shorten hospitalization time and reduce the occurrence of nursing adverse events.