The position competency of students majoring in health information management was assessed by inves-tigating its trainees, graduates, related staff and experts with questionnaires according to the position competency theory, based on which, the basic health information management curriculum system framework was constructed with certain suggestions put forward for it.

Objective To study the correlation among acute cerebral infarction area ,serum high sensitivity C reactive protein(hs‐CRP)andhomocysteine(Hcy)concentrations.Methods 112acutecerebralinfarctionpatientsreceivedinneurologicaldepartment of the hospital were enrolled in the study .They were divided into small infarction size group(n=56) and large infarction size group (n=56) ,according to the cerebral infarction area determined by using MRI and MRA .Common information of patients such as age , sex ,smoking ,drinking ,hypertension ,hyperlipidemia and diabetes history were recorded ,serum hs‐CRP and Hcy concentrations were also determined .The comparison between the 2 groups were performed on those common information and test results .Results There was no statistically significant difference on age ,alcohol consumption ,smoking ,hypertension and high cholesterol between the two groups(P>0 .05) .Serum concentrations of hs‐CRP ,Hcy and blood glucose in small infarction size group were lower than those in large infarction size group(P<0 .05) ,and the infarct areas were positively correlated with hs‐CRP ,Hcy and blood glucose levels(r=0 .625 ,0 .833 ,0 .651 ,P<0 .05) .Conclusion Serum hs‐CRP ,Hcy level are high risk factor for atherosclerosis plaque for‐mation of acute cerebral infarction patients ,the serum levels help predict the infarction area ,and diabetes is an important cause of cerebral infarction .

Cochlear Implantation ; instrumentation ; Cochlear Implants ; Electrodes ; Female ; Humans ; Infant ; Prosthesis Failure

Breast Neoplasms ; classification ; genetics ; metabolism ; pathology ; Chromosome Aberrations ; Female ; Gene Deletion ; Humans ; Phyllodes Tumor ; classification ; genetics ; metabolism ; pathology ; Twist-Related Protein 1 ; metabolism ; Wnt Signaling Pathway

Purpose The aim of this study was to reveal the role of SGT,the small glutamine-rich tetratricopeptide repeat (TPR)-containing protein,in the developing mouse brain through examining the expression profile of SGT during the development stage. Methods In this study, quantitative RT-RCR and Western blot were applied to investigate the expression of SGT mRNA and protein in the mouse whole brain. Western bolt was also used to detect the expression of SGT in the hippocampus, cerebral cortex, striatum and cerebellum. Immunohistochemical analysis on postnatal and adult mouse brain was performed to examine the subcellular localization of SGT. Results Our data showed that the levels of SGT mRNA and protein in the mouse whole brain were both high during the postnatal stage and declined in the adult. Regional expression of SGT protein in the hippocampus, cerebral cortex, striatum and cerebellum showed a similar expression profile.Immunohistochemical analysis found that in the P14 mouse brain, SGT was abundant in all the CA regions of hippocampus as well as most regions of cerebral cortex and striatum. In the cerebellum, SGT was mainly distributed in Purkinje cells. In the sections of the adult mouse brain, faint expression was observed in the regions mentioned above. Conclusions Our findings firstly exhibit the expression pattern of SGT in the mouse brain development,which might shed new light on further functional analysis of SGT in the central nervous system.

BACKGROUND: Whole blood viscosity is the keystone in hemorheological research, which has important significance in the exposure of the generation of ischemic diseases including myocardial infarction (MI) and cerebral infarction(CI) due to hemorheological changes.OBJECTIVE: To investigate the characters of macroscopical and microscopical hemorheology to explore the standardization of hemorheological measuring method.DESIGN: An observatory comparative study based on the venous blood of healthy individual.SETTING: Department of physics in a medical college.MATERIALS: Venous bloods of 86 healthy individuals were randomly selected for the study, which was conducted in the Laboratory of Hemorheology of Guilin Medical College.METHODS: Totally 86 whole blood samples were tested under 7 shear rates (0.6 s-1,2 s-1,5 s-1, 10 s-1, 20 s-1, 40 s-1, 100 s-1) from low shear rate to high shear rate, and then from high shear rate to low shear rate. The measuring value of the two processes was compared.Casson viscosity and yield stress in two measuring process.RESULTS: There was no significant difference of apparent viscosity( P＞ 0.05 ) except the 0.6 s- 1 -corresponding apparent viscosity ( P ＜ 0.05 )at some shear rate in two measuring process. There was no significant difference in Casson viscosity and yield stress( P ＞ 0.5, P ＞ 0. 3).CONCLUSION: The measurement of Casson viscosity and yield stress is good for the optimization of the standardization of hemorheological indicators and measuring method; and it is feasible to test apparent viscosity, Casson viscosity and yield stress repeatedly in same blood sample, which has important significance in the exposure of the hemorheological changes and the generation and development mechanism of ischemic diseases including MI,CI, hypertension, and coronary heart disease(CHD).

Objective To assess whether the plaque volume of carotid artery evaluated by three-dimensional ultrasound can predict the risk of cardiovascular events earlier in patients with coronary heart disease, in comparison with plaque thickness.Methods A total of 99 patients were enrolled, including 66 patients with asymptomatic carotid plaque and 33 patients with coronary heart disease (CHD) and carotid plaque.Three-dimensional ultrasound was performed in all the patients.Coronary angiography was performed in all the patients in CHD group.Coronary artery Gensini score was calculated.Plaque thickness, plaque area and plaque volume were compared between the two groups.We analyzed the relationship of plaque volume with Gensini score and other cardiovascular risk factors.Results Plaque volume was significantly higher in CHD group [(1312.38±513.70)mm3] than in asymptomatic group [(947.54±321.41)mm3] (P=0.023).However, plaque thickness and plaque area were not significantly different between the two groups (P>0.05).Plaque volume was positively correlated with Gensini score (r=0.519, P=0.002), homocysteine (r=0.569, P=0.002), and cardiovascular risk factors such as diabetes, blood glucose and glycosylated hemoglobin.There was a negative correlation between plaque volume and high-density lipoprotein (HDL), a factor protecting against cardiovascular events (r=-0.387, P=0.038).Conclusion Compared with plaque thickness, the volume of carotid plaques assessed by three-dimensional imaging technique may be a better predictor of the risk of cardiovascular events in patients with coronary heart disease.

Objective To investigate the potential roles of 14 -3 -3 in the pathogenesis of the cervical cancer.Methods Tissues from 107 patients with cervical cancer(cervical cancer group),50 cases of cervical intra-epithelial neoplasia (cervical intraepithelial neoplasia group)and 50 cases of chronic cervicitis (chronic cervicitis group)were obtained.Immunohistochemistry was performed to determine the expression of 14 -3 -3 protein in tissues of the three groups.Results The positive rate of 14 -3 -3 in cervical cancer group(75.70%)was signifi-cantly higher than that in cervical intraepithelial neoplasia group and chronic cervicitis group(42.00%,30.00%, respectively)(χ2 =17.00,29.96,all P <0.01),while there was no significant difference of 14 -3 -3 level between the cervical intraepithelial neoplasia group and chronic cervicitis group(χ2 =1.56,P >0.05).Moreover,in the cervi-cal cancer group,the positive rate of 14 -3 -3 in lymph node metastasis group was 88.89%,which was higher than that in negative lymph node metastasis group (63.16%)(χ2 =5.15,P <0.05).Conclusion The expression level of 14 -3 -3 protein in cervical cancer is increased,it may be correlated with cervical cancer cell malignant differenti-ation and metastasis.Perhaps,14 -3 -3 will play a key role as a novel target for tumor therapy.

Objective To generate a prokaryotic expression system of Salmonella paratyphi A nmpC gene that encoding an outer membrane protein(OMP),and to determine immunogenicity and immonuprotection of the recombinant expressed product rNmpC and carrying and expression frequencies of the nmpC genes in isolates of S.paratyphi A.Methods A nmpC gene clone was obtained from a clinical S.paratyphi A strain JH01 by PCR and T-A cloning method,and then a prokaryotic expression system of the gene clone was generated.SDS-PAGE and Bio-Rad Agarose Image Pattern Analysis System were applied to examine the expression and yield of rNmpC.Antigenicity and immunoreactivity of rNmpC were determined by immunodiffusion test,Western blot assay and micro-Widal's test.The carrying and expression rates of nmpC genes in 98 S.paratyphi A isolates were detected by PCR and ELISA.By a mouse infection model,the immunoprotective effect of rNmpC against the lethal challenge of S.paratyphi A was determined.Results All the cloned nmpC genes had 100% nucleotide and putative amino acid sequence identities compared to the reported sequencing data.The expression yield of rNmpC was approximately 30% of the total bacterial proteins.rNmpC could efficiently induce rabbits to produce specific antibody and present positive Western hybridization signals with S.paratyphi A antiserum.All the tested S.paratyphi A strains have nmpC gene as well as express NmpC protein,but no nmpC gene could be detectable in S.typhi,S paratyphi B and S paratyphi C.Immunization with 100 μg and 200 μg rNmpC contributed 41.7%(5/12) and 66.7%(8/12) immunoprotective rates in mice,respectively.The sera from rNmpC immunized mice and survival mice in the NmpC is an unique OMP antigen of S.paratyphi A with conserved sequence,extensive distribution and natural expression.This OMP can be used as one the candidate antigens for developing multiple-valence genetic engineering vaccine of S.paratyphi A based on its fine immunogenicity and certain immunoprotection.

Objective To explore the preventive effect of early bowel intervention on the gastrointestinal dysfunction in patients with invasive mechanical ventilation.Methods Sixty patients with invasive mechanical ventilation were equally randomly divided into the observation group and the control group.patlents in the control group were treated by conventional nursing care,while Patients in the observation group were given the early bowel intervention including abdominal massage and stimulation of rectum.The two groups were compared in terms of gastrointestinal dysfunction.Result The rate of gastrointestinal dysfunction in the observation group was significantly lower than that of the control group(P<0.05).Conclusion The early bowel intervention may effectively promote intestinal peristalsis,protect the intestinal digestion, absorption of nutrients and mucosal barrier function and prevent gastrointestinal dysfunction in patients with invasive mechanical ventilation during enteral nutrition.