The gene of preferentially expressed antigen of melanoma(PRAME) is a gene marker of leukemia,which can be used to monitor the minimal residual disease,and can make us to comprehend the curative effect,prognosis,the grade of risk and can predict relapse.In addition,PRAME as a human tumour antigen,can be recognized by autologous cytolytic T lymphocytes,is a ideal target of immunotherapy in leukemia,and investigate the methylation level of PRAME gene CpG island,which can help us comprehend the relation of the mechanism of PRAME and leukemia.

Objective: To summarize the major progress in medical image registration in recent years. Furthermore, based on the recent advances in this field, this paper can provide a reference in following domains: three-dimensional medical image reconstruction, medical image visualization, quantitative analysis. Methods: Firstly, referring to a myriad of latest papers on medical image registration. Secondly, analyzing traits and exiting problems of techniques which presented in those papers. Finally, putting forward some efficient methods for solving these problems. Results: This paper compares the characteristics of some typical algorithms and its application and looks forward to the future research work. Conclusion: Using optimization strategy to improve the quality of image registration and studying on non-rigid image registration are the directions for future research in medical image registration field.

OBJECTIVE To analyze pathogen distribution of lower respiratory tract infection after tracheotomy in patients with severe brain injury to offer prevention and control measures.METHODS The data of patients with severe brain injury after tracheotomy were collected and analyzed in our hospital from Jun 2005 to Dec 2005,Bacteria culture were analyzed in 74 cases twice a week.RESULTS Eighty-four strains of pathogens were identified.Gram-negative bacilli,Gram-positive cocci and fungi accounted 73.04%,11.23% and 15.73%,respectively.Through control measures,the infection rate was reduced from 90% in 2004 to 48.65%.CONCLUSIONS The infection rate of patients with severe brain injury can be reduced through effective methods of prevention and control.

To explore the expression and function of MG 7 Ag on gastric cancer cell line SGC7901 and its drug resistant subline,SGC7901/VCR, with different resistance index (0 3, 0 7, 1 0) The expression of MG 7 Ag on SGC7901 and SGC7901/VCR cells was determined by immunohistochemical method and flow cytometry (FCM) MTT assay was used to explore the effects of monoclonal antibody MG 7 on drug sensitivity of gastric cancer cells FCM was used to explore the effects of MG 7 on adriamicine accumulation of SGC7901/VCR cells SGC7901/VCR cells exhibit increasing positive staining of MG 7 than SGC7901, according to the increasing resistance index Sensitivity of vincristine of both SGC7901 and SGC7901/VCR cells was reduced after being treated with MG 7 And SGC7901/VCR cells showed decreased adriamicine accumulation when they were incubated with MG 7 Therefore, the conclusion is that MG 7 Ag may be a novel molecule associated with multidrug resistance of gastric cancer, and play an important role in maintaining the drug resistant phenotype of SGC7901/VCR cells.

Objective To evaluate the effects of fentanyl transdermal system on intractable postherpetic neuralgia .Methods 30 patients(after 1 course of conventional drugs with nerve block therapy ,VAS score>5 points)were randomly divided into tramadol group(group T ) and fentanyl group(group F) ,15 cases in each group .Results VAS of the two groups decreased after treatment (P<0 .05) ,group F decreased more significantly(P<0 .05) .The quality of life scores after treatment in two groups were signifi-cantly increased(P<0 .05) ,group F scoring was higher(P<0 .05) .The incidence of side reaction ,nausea and vomiting was lower in group F(P>0 .05) .The incidence of dysuria was 26 .67% in group T ,and there was no dysuria in group F(P<0 .05) .Constipation of group T was 40% after treatment(P<0 .05) .The incidence of somnolence of group F was higher than T group(P<0 .05) .The two groups were no respiratory depression .Conclusion Fentanyl transdermal system for intractable postherpetic neuralgia has good analgesic effect ,less side effect ,and well-tolerated .

Objective To study the effects of Celastrus orbiculatus extracts (COE) on the expression of VEGF and CECs of Hepal-6 tumor bearing mice.Methods Animal Models of Hepal-6 portability liver tumor bearing mice were established and randomly assigned to six groups (eight mice per group) as follows:untreated control group,solvent vehicle control (DMSO) group,physiological saline control group,cisplatin-treated group,and different dosages COE-treated groups (40 mg/kg,20 mg/kg,10 mg/kg).To approach the effects of COE on the expression of VEGF and CECs in Hepal-6 toumor bearing mice.Results The ELISA and flow cytometer showed that COE could significantly inhibit the expression of VEGF(45.24±21.36) pg/ml and CECs (0.83 ± 0.10) ％ compared with the physiological saline control group (P＜ 0.01 ) with the dose of 20mg/kg.Conclusion COE could significantly down-regulate the expression of VEGF and CECs.It may be related with the effect of COE in inhibiting angiogenesis of tumor.

ObjectiveTo study the characteristics of nonfunctioning parathyroid cysts (NFPTC) for its diagnosis and treatment.Methods 5 cases of NFPTC were hospitalized during 1985～1999. The clinical features, serum calcium evaluation ultrasonography and nuclide scanning were performed. Postoperative pathologic and immunihistochemistry detection in NFPTC were made.ResultsTumours in the 5 cases were removed by operation and diagnosis was verified by pathology. Tumors were all positive for chromogranin A (CgA),synaptophysin(Syn),and neuron specific enolase(NSE). Follow up found no recurrence. ConclusionsSurgical resection is most effective in treating NFPTC and diagnosis is made by pathologic examination.

Objective Investigate the relation between decrease of apoptosis caused by delayed preconditioning and expression of SMAC and XIAP.Methods Sprage-Dawleyt rats were divided into four groups: control,sham,I/R and IPC/SWOP.The rats in I/R group underwent ischemia for 1 hour by classic artery ligation and reperfusion for 1 hour.The rats in IPC/SWOP group underwent tree cycles of 5-minute ischemia and 5-minute reperfusion 24 hours prior to the index occlusion.Cell apoptosis was measured by flow cytometry,the activity of caspase-3 was also measured.The expression of SMAC and XIAP in cytosol of myocardial cell was measured by Western blot.Results Cell apoptosis rate,activity of caspase-3 and expression of SMAC significantly increased in I/R as compared with control(P

Objective:Combining the reality of school, to explore the method of training on the implementation of the outstanding doctor education.Methods: Deepening the reform of teaching, curriculum integration, constructing reasonable curriculum system, strengthen the innovation ability and practice ability, build a outstanding doctor training system, revision of the talent training scheme, the implementation of outstanding physician education program.Results:in our school outstanding doctor the actual situation of the education training, and further analysis and research, explore the method of training on the implementation of the outstanding doctor education.Conclusion: by deepening the reform of teaching, constructing curriculum system, strengthen the innovation ability and practice ability, build a outstanding doctor training system, revision of the talent training scheme, the implementation of outstanding physician training plan, so the method of training on the implementation of the outstanding doctor education is feasible.

Objective To investigate the diagnosis and treatment of cerebral venous sinus thrombosis ( CVST) in early pregnancy.Methods The clinical data of 4 patients with CVST in early pregnancy were analyzed retrospectively.Results The age of the 4 patients with CVST during early pregnancy was 22 -28 years old.The clinical symptom was headache, and 1 case with seizure.The clinical sign was papilledema.The MRI and MRV examination showed that the superior sagittal sinus thrombosis in 1 case, the superior sagittal sinus and left transverse sinus thrombosis in 1 case, and the left transverse sinus and sigmoid sinus thrombosis in 2 cases.The patients were received anticoagulant therapy with low molecular weight heparin, 1 case was added endovascular thrombolytic therapy.After therapy, 2 cases were cured, and 2 cases improved.Conclusions CVST in early pregnancy is easy to be misdiagnosed.MRI and MRV are helpful to diagnose it.The mainly therapies are endovascular thrombolysis and anticoagulation therapy with low molecular weight heparin.