1.Study Progress on Embryonic Stem Cells and Adult Stem Cells Derivated into Male Germ Cells
Journal of Applied Clinical Pediatrics 2004;0(08):-
Embryonic stem cells(ESCs),a kind of totipotent cells,can differentiate into cell type of the 3 germ layers.Adult stem cells(ASCs) are pluripotent stem cells,which can self-renew and transdifferentiate into a completely different tissue derivative cells.Thus ASCs can transdifferentiate into germ cells in a certain condition.Great progress has made for the study on human ESCs.However,use of ESCs faces the immunological rejection,oncogenicity and ethical controversies problems,and these limit their uses for treatment of human diseases.On contrast,ASCs can advoid these problems,and they may have a better perspective.Germ cells as carriers of genetic materials,are worth of studying.If male germ cells can be derived from ASCs,this will open the possibilities for the use of these cells for male infertillty in clinical application.In this review,we will introduce the progress on derivation of male germ cells from ESCs and ASCs.
2.Observation of Erythropoietin Acrossing Blood - Brain Barrier of Premature Infants
ying-hong, WANG ; chang-lian, ZHU
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To observe whether recombinant human erythropoietin (rhu- EPO) could cross blood- brain barrier (BBB) of premature infants. Methods Thirty - six premature infants, with gestational age 28 - 35 weeks, birth weight0.05).Conclusion Rhu- EPO can cross the BBB of premature infants.
4.Comparison of China-and Germany-made Electromagnetic Lithotripters for Upper Urinary Tract Calculi:Preliminary Report of Perspective Study
Huibo LIAN ; Hongqian GUO ; Ying WANG
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To perspectively compare the clinical outcomes of China-and Germany-made electromagnetic lithotripters in the treatment of upper urinary tract calculi.Methods Between January 2005 and August 2005,60 cases of upper urinary tract calculi were randomly divided into two groups:China-made and Germany-made groups(30 in each).Extracorporeal shock wave lithotripsy(ESWL)was performed by a same group of surgeons.Results The stone-free rate was 73%(22/30)in China-made group,and was 90%(27/30)in Germany-made group 3 weeks after ESWL(?2=2.283,P=0.095);and was 100% in both the groups 3 months postoperation.The retreatment rate was 27%(8/30)in China-made group,and 10%(3/30)in Germany-made group(?2=2.283,P=0.095).The rate of auxiliary procedure in both the groups was 0.The efficacy quotient(EQ)was 0.79 in China-made group,and was 0.91 in Germany-made group.Totally,39 ESWLs were performed on the patients in China-made group,while 33 ESWLs were carried out in Germany-made group.The rates of macroscopic hematuria,skin injury,and renal colic were 87%(34/39),64%(25/39),and 8%(3/39)respectively in China-made group,and were 91%(30/33),6%(2/33),and 6%(2/33)in Germany-made group(?2=0.016,P=0.900;?2=25.693,P=0.000;and ?2=0.000,P=1.000;respectively).One patient in China-made group developed fever after ESWL,while no one in Germany-made group had the symptom.In both the groups,no patients had hematuria,renal edema,or subcutaneous perinephric hemorrhage.Conclusion Both China-and Germany-made electromagnetic lithotripters are effective,safety,and non-invasive.
5.Efficacy of rituximab combined with CHOP chemotherapy for 35 patients with aggressive B cell non-Hodgkin’s lymphoma
Ying LI ; Meijun LI ; Ming LIAN
Chinese Journal of Biochemical Pharmaceutics 2015;(12):168-170
Objective To analyze efficacy of rituximab combined with chemotherapy for 35 patients with aggressive B cell non-Hodgkin’s lymphoma.Methods 70 patients with aggressive B cell non-Hodgkin’s lymphoma were chosen from Hematology Department of First Affiliated Hospital of Liaoning Medical University and divided into observation group and control group at random, each group with 35 cases.The control group were treated with CHOP chemotherapy, and the observation group were treated with anti-CD20 monoclonal antibody rituximab combined with CHOP chemotherapy. The efficacy and toxicity of two group were compared.Results The total effective rate of observation group was significantly higher than that of control group (80.0%vs.37.1%;χ2 =13.246,P<0.001).Fever cases of observation group were significantly more than those of control group (15vs.4;χ2 =8.741,P=0.003), while the other adverse reactions of leucopenia and gastrointestinal reaction between two groups were not significantly different. Conclusion For patients with aggressive B cell non-Hodgkin’s lymphoma, rituximab combined with CHOP chemotherapy has better short-term effects and its adverse reactions are tolerable, which helps patients to improve the quality of life.
6.Effect of iohexol on the renal function in children undergoing cardiac angiography.
Xin-Ying ZHANG ; Yu-Lin WANG ; Xue-Lian ZHU
Chinese Journal of Contemporary Pediatrics 2009;11(11):935-936
Child
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Child, Preschool
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Contrast Media
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adverse effects
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Coronary Angiography
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Female
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Humans
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Infant
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Iohexol
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adverse effects
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Kidney
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drug effects
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physiology
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Male
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beta 2-Microglobulin
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urine
8.Case of ophthalmalgia caused by facial paralysis.
Qi-Tai LI ; Lian-Ying LU ; Yan-Yun LIU
Chinese Acupuncture & Moxibustion 2011;31(8):768-768
9.Preliminary study of the ultrasonographic imaging of urogenital hiatus in late pregnancy women
Qin LI ; Tao YING ; Lian XU ; Feifei LIU ; Bing HU
Chinese Journal of Ultrasonography 2012;21(6):496-499
ObjectiveTo observe the morphology and structure of the urogenital hiatus in late pregnancy women using transperineal three-dimensional (3D) pelvic floor ultrasonography.Methods Twenty five nulliparas and fifty late pregnancy women were examined by transperineal 3D pelvic floor ultrasonography.The images of urogenital hiatus were obtained and compared to study their differences between nulliparas and late pregnancy women.Results Compared to nulliparas,urogenital hiatus in late pregnancy women tended to be circular and with an offset position.The puborectalis were bended and could be avulsed.Pelvic floor connective tissue could be loose.The morphology of vaginal might be abnormal and pelvic organ might prolapsed slightly.ConclusionsThe morphology and structure of urogenital hiatus in late pregnancy women are different to those in nulliparas,the hiatus tend to be slackness.3D pelvic floor ultrasonography is an effective imaging method to observe the morphology and structure of urogenital hiatus in pregnancy women.
10.Investigation of Tryptophan in Gastric Juice by High Performance Liquid Chromatography Coupling with Ultraviolet,Fluorescence Detection and Mass Spectrometric Identification
Dongjie MA ; Xu XU ; Wei LIAN ; Ying CHEN ; Yunlin WU
Chinese Journal of Analytical Chemistry 2009;37(11):1617-1621
The HPLC-UV-Fluorescence technique and HPLC-UV-MS technique were used to determine fluorescence molecule in gastric juice of patients with malignant gastric cancer,which is identified as tryptophan. Lidocaine was detected in gastric juice of patients who were examined by gastric endoscopy. A method was developed to determine concentration of tryptophan and lidocaine in gastric juice using HPLC-UV technique,in which Kromasil C_(18) column(150 mm×4.6 mm,5μm) and methanol(containing 0.1% trifluoroacetic acid (TFA))-water(containing 0. 1% TFA) as gradient mobile phase were used. The linear relationships were obtained between the concentration of tryptophan in the range of 0.5 - 200 mg/L and its peak area at 278 run with correlation coefficient of 0.9994,and between the concentration of lidocaine in the range of 20 -5000 mg/L and its peak area at 254 nm with correlation coefficient of 0.9992. The limit of detection is 0.15 mg/L for tryptophan and 5 mg/L for lidocaine. The recovery is 70. 8% - 110. 4% for tryptophan and 87. 1% -116.2% for lidocaine. 38 gastric juice samples of patients with malignant gastric disease and 48 gastric juice samples of patients with benign gastric disease were tested and the concentration of tryptophan in those two groups of gastric juice samples was apparently different.