AIM: To investigate the effect of Haikun Shenxi Capsule(Fucoidan) on tubule function in chronic kidney disease(CKD) patients. METHODS: One hundred and one patients with CKD 2、3 stage were randomly divided into two groups dom: control group were given routine therapy and therapy group were given routine therapy plus Haikunshenxi Capsule.We observed the changes of the level of UOP、NAG、?_1-MG in two groups. RESULTS: The level of NAG、?_1-MG significantly decreased,urine osmoticpressure significatly increased(P0.05). CONCLUSION: Haikun Shenxi Capsule has a protective effect on tubule in CKD patients.

Objective:To investigate the protective effect of activated AMP-activated protein kinase(AMPK) on the human umbilical vein endothelial cell(HUVEC) damage induced by palmatic acid(PA) and the related mechanism.Methods: HUVEC were divided into 8 groups according to the culture media(cultured for 24 to 72 h): blank control(conventional culture),300 ?mol/L PA,1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR),PA+AICAR,2 mmol/L metformin,PA+metformin,10 ?mol/L pioglitazone,and PA+pioglitazone groups.Survival rates of HUVEC were determined by MTT at 24,48 and 72 h after culture.Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture.Results: Compared with blank control group,cell survival rates in PA group were decreased by 58.95%,36.68% and 15.09% at 24,48 and 72 h(P

For a long time, people have different ideas about whether asthmatic children can participating in sports as others and how to exercise they need. As restricted by the possibility that exercise may induce asthma, the level of asthmatic children' s exercise is lower than their classmates. Research in recent years in abroad have showed that exercise is not only helpful to children's growth and development psycho`gicat health,but also helpful to asthmatic children's sympton control, reducing asthma exacerbation, reducing medication use and impoving their quality of life, and asthmatic children can exercise safely as long as been managed appropriatly. Scholars have paied more and more attention to the importance of exercise in children asthma' s management, and exercise prescription had been recommended as an important part of children asthma' s management in abroad.

Objective: To investigate the protective effect of activated AMP-activated protein kinase (AMPK) on the human umbilical vein endothelial cell (HUVEC) damage induced by palmatic acid (PA) and the related mechanism. Methods: HUVEC were divided into 8 groups according to the culture media (cultured for 24 to 72 h): blank control (conventional culture), 300 μmol/L PA, 1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR), PA+AICAR, 2 mmol/L metformin, PA+metformin, 10 μmol/L pioglitazone, and PA+pioglitazone groups. Survival rates of HUVEC were determined by MTT at 24, 48 and 72 h after culture. Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture. Results: Compared with blank control group, cell survival rates in PA group were decreased by 58.95%, 36.68% and 15.09% at 24, 48 and 72 h (P<0.05), respectively. Seventy-two hours after culture, HUVEC survival rates of AICAR+PA group, Met+PA group and PGZ+PA group were obviously higher than that of PA group (P<0.05); AICAR, metformin or pioglitazone alone had no obvious influence on HUVEC survival rate. Twenty-four hours after culture, Phospha-AMPK expression was increased in AICAR, metformin and pioglitazone groups compared with that of control group (P<0.05). Conclusion: AICAR, metformin and pioglitazone can decrease the damage of HUVEC induced by PA, possibly through activating AMPK.

Objective:To analyze the analgesic effectiveness of dezocine after laparoscopic cholecystectomy. Methods: Totally 80 patients with laparoscopic cholecystectomy were randomly divided into the observation group and the control group. The control group was given tramadol 0. 2 mg·kg-1 for anesthetize, the observation group was given dezocine 0. 1 mg·kg-1 analgesia. Conducted according to a simgle drug additional pain status of the patient. After the surgery, the time of surgery, VAS and BCS pain condition score of the pa-tients were analyzed and compared, and the incidence of adverse reactions of the two groups were recorded. Results:After the surgery, the operation time, opening eyes, awaking time and extubation time showed no significant difference in the two groups (P>0. 05). VAS scores of the observation group in 30min, 1h, 2h and 6h after the surgery were significantly lower than those of the control group, and BCS score was significantly higher than that of the control group (P<0. 05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group (χ2 =4. 020 5,P=0. 045 0). Conclusion:The analgesic effect of dezocine after lap-aroscopic cholecystectomy is promising, which can significantly alleviate the pain after the operation and is worthy of wider application.

Objective To assess the feasibility and safety between ALPPS and two-stage hepatectomy (TSH).Methods An electronic search was performed of the MEDLINE,EMBASE using subject heading to identify nearly three-years articles published that related to this topic.Pooled odds ratio was calculated for binary data and mean differences for continuous outcomes,using fixed-effects and random-effects models for meta-analysis.Results Four studies involving a total of 312 patients were used in the analysis.We found that ALPPS produced a higher increase rate of FLR than TSH (MD =24.78;95％CI:0.63 to 48.94;P =0.04).Comparing with TSH,ALPPS produced a shorter FLR growth time (MD =-26.55;95％ CI:-37.13 to -15.97,P ＜ 0.05).There was no statistical significance in overall mortality (OR =2.43;95 ％ CI:0.94 to 6.31;P =0.07),while ALPPS produced a more severe complication rate (≥ Ⅲ b) than TSH (OR =2.47;95 ％ CI:1.14 to 5.36;P =0.02).Conclusions It was better to make the FLR increasing to safe resection for ALPPS than TSH in a short period of time.There was no statistical significance in overall complication and mortality between ALPPS and TSH,but ALPPS produced more severe complication rate (≥ Ⅲb) than TSH.

Objective To investigate the hemostatic effect of balloon compression on the puncture site in cancer patients with peripherally inserted central catheter (PICC) inserted by ultrasound-guided modified Seldinger technique (MST). Methods Between October 2015 to January 2016, 50 cancer patients who accepted PICC inserted by ultrasound-guided MST were assigned into test group and control group respectively according to the admission time.Each group contained 25 cases. Balloon compression method was used to stop bleeding of the puncture point in the test group, while bandage compression hemostasis adopted in the control group. Results The incidence of bleeding on puncture site in the last 24 h of PICC placement was 28% (7/25) in the test group, while 68% (17/25) in the control group, and there were statistical differences between the groups (Z=-2.653,P<0.01). The incidence of bleeding on puncture site in 24-72 h of PICC placement was 16% (4/25) in the test group, while 36% (9/25) in the control group, and the difference had statistical significance (Z=-1.679,P>0.05). The incidence of limb swelling or numbness in the test group was 8% (2/25),which was lower than 32% (8/25) in the control group, and the difference had statistical significance (χ2=4.5,P < 0.05). Conclusions Balloon compression method can reduce the bleeding ratio of puncture site in cancer patients with PICC inserted by ultrasound-guided MST,and can relieve limb swelling or numbness .It is simple and safe in operation and can be easily accepted by the patients .

Objective To analyze the test results of hepatitis B virus surface antigen(HBsAg) ,alanine aminotransferase(ALT) and anti-hepatitis C virus (HCV) ,anti-human immunodeficiency virus (HIV) ,anti-treponema pallidum (TP) antibodies among voluntary blood donors ,and to provide basis for recruiting low-risk voluntary blood donors and reducing blood abandonment . Methods 551 133 blood samples derived from voluntary blood donors which had accepted enzyme-linked immunosorbent assay (ELISA) screening in Chongqing Blood Center from 2008 to 2012 were collected and were subject to HBsAg ,ALT and anti-HIV , anti-HCV ,anti-TP antibodies detection .Laboratory information management system ITSWELL was employed to read ,save and gather the test results ,and whether the samples were qualified was determined .Results Total of 37 534(6 .81% ) substandard blood samples were detected .Among them ,the substandard rates of HBsAg ,ALT and anti-HCV ,anti-HIV ,anti-TP antibodies were 1 .10% ,3 .79% ,0 .51% ,0 .33% and 1 .08% ,respectively .Conclusion Strengthening the screen of blood detection reagents and building the team of voluntary blood donors will contribute to the safety of clinical blood transfusion .

AIM: To evaluate the efficacy of simvastatin on exercise-induced myocardial ischemia in patients with stable exertional angina pectoris and hypercholesterolemia. METHODS: Ninety patients with stable exertional angina pectoris, positive rate of exercise test and hypercholesterolemia were randomly divided into two groups: the therapy group assigned to a 12-week treatment period either diet plus simvastatin (n=45); the control group with diet alone (n=45). All the patients underwent exercise testing before and after the treatment period. RESULTS: (1) Simvastatin-treated patients had a significant variation in total cholesterol, triglyccrides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol after treatment (P

Acupuncture Therapy ; Apoptosis ; Brain Ischemia ; pathology ; therapy ; Humans