Objective:To discuss the applicative value of 1.5TMR-diffusion weighted imaging (DWI) sequence and apparent diffusion coefficient (ADC) measurement in the diagnosis and differential diagnosis of endometrial carcinoma.Methods: Thirty seven cases were collected in endometrial carcinoma group, 11 cases were selected in benign group and other 20 normal volunteers. Data of the DWI and ADC value, common-MRI(c-MRI) sequence in the 48 patients and 20 normal volunteers was contrasted and analyzed.Results: The differences between endometrial malignant tumors, benign lesions and the normal have statistical significance.Conclusion: 1.5 T MR-DWI sequence and ADC value has a superior ability in detecting endometrial lesions. It can be used to differentiate the endometrial carcinoma from either benign or normal cases, it can provide reliable assistance in the clinical cure.

Circulating tumor DNA (ctDNA) is emerging as a novel biomarker for tumor evaluation, offering advantages such as high sensitivity and specificity, minimal invasiveness, and absence of radiation. Currently, various techniques including gene sequencing and PCR are employed for ctDNA detection. The utilization of ctDNA for monitoring minimal residual disease (MRD) enables comprehensive assessment of tumor status and early identification of tumor recurrence, achieving a remarkable detection sensitivity of 0.01%. Therefore, ctDNA holds promise as a biomarker for early diagnosis, treatment response monitoring, and prognosis prediction in solid tumors. This article reviews the commonly used methods for detecting ctDNA and their advantages in evaluating tumor MRD and guiding clinical diagnosis and treatment.
Humans ; Circulating Tumor DNA/genetics* ; Neoplasm, Residual/genetics* ; Biomarkers, Tumor/genetics* ; Neoplasm Recurrence, Local ; Prognosis

Despite that survival rates have been improved in childhood malignant solid tumors,progres sion during treatment or relapse after complete remission remain a major cause hindering patients from getting long-time survival.Minimal residual disease(MRD) is closely related to relapse of solid tumors.This review discusses the advance and value of various methods used in MRD detection,aiming to establish a stable and reliable method for MRD detection.

Objective:To prepare vascular endothelial growth factor receptorⅡ ( VEGFRⅡ)-mediated targeted sulphur hexafluo-ride microbubble image agent and evaluate its targeting ability in human breast cancer MCF-7 in vitro. Methods:The sulphur hexafluo-ride microbubble image agent was prepared by a membrane dispersion-ultrasonic method and optimized by Box-Behnken design using design-expert 8. 0. 6 software. VEGFRⅡ-mediated targeted microbubble image agent was prepared by an absorption method. VEGFRⅡ-mediated targeted microbubble image agent was characterized by the morphology, particle size distribution and zeta potential. The targeting ability of the image agent in human breast cancer MCF-7 in vitro was detected by an immunofluorescent assay. Results:The particle size, polydispersion index and zeta potential of VEGFRⅡ-mediated targeted microbubble image agent was (3. 81 ± 0. 32) μm, 0. 261 ± 0. 037 and ( -25. 7 ± 2. 8) mV, respectively. The microbubbles were small and spherical with smooth surface as seen under a transmission electron microscope. VEGFRⅡ-mediated targeted microbubble image agent could combine with human breast cancer MCF-7 specifically in vitro. Conclusion: The absorption preparation technology of the targeted microbubble image agent is feasible, and the microbubble image agent has strong and especial targeting function in vitro.

Three methods to become a member of PubMed Commons and the ways to add, reply and share reviews using PubMed Commons were described in order to help its users to master how to use it, and the significance of PubMed open reviews was pointed out.

Rotavirus is the most common cause of infantile diarrhea in autumn and winter.Rotavirus not only induces severe gastrointestinal symptom,but also arise multisystem complication,such as circulatory system,nervous system,respiratory system.Mastering multisystem damage mechanism of rotavirus has important significance to our future treatment.Not only we should correct severe dehydration,acidosis and electrolyte disorder,but protect important organ functions.

The influencing factors of EPS produced from LAB were studied by using the Phenol-H_(2)SO_(4 )method. It was demonstrated that the strain produced EPS at the most amount when it was incubated to 40~48h and pH4 under 30℃. Glucose is the most suitable sugar source for LAB producing EPS. The rough EPS was obtained from LAB' culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed of two polysaccharides that were different completely from the molecule weight and the amount. The purified EPS was got through the SephadexG-200 column and it was showed that it was a purified sample by thin layer chromatograghy.

Objective:To investigate the frequency and antihiotic-resistance of inducible-clindamycin-resistant Staphylococci for rational prescription of antibioties. Methods:Erythromycin-inducible-Clindamycin-resislant staphylococci were detected by using D test recommended by Clinical Laboratory Standard Institution(CLSI/NCCLS), and the resistance of meihicillin-resisiant Staphylococcus and Methicillin-sensitive Staphylococcus to 12 kinds of antibioties were detected. Results:Among 310 strains of Staphylococci, in strains of erythromycin-resistani-and-clindamyein-sensitive Staphylococci, the percentage of MRS with positive D test was 6.6%(12/183), and that of MSS was 18.1%(23/127). There is significant difference between them(x~2=9.99, P

Bronchi ; surgery ; Bronchoscopy ; Child ; Foreign Bodies ; surgery ; Humans ; Infant ; Pneumothorax ; Trachea

Objective To study the impact of unilateral spatial neglect (USN) on the rehabilitation outcome of stroke patients with hemiplegia. MethodsNinety-three stroke patients with hemiplegia were treated with routine interventions. Of the 43 patients with USN, 22 patients served as an experimental group and received intervention for USN, and the other 21 patients served as an observation group and were not intervened for USN. The remained 50 patients without USN served as a control group. All the patients were evaluated by Barthel index(BI) before and after rehabilitation treatment to assess their performance in activities of daily living (ADL). ResultsBefore treatment, the patients in the experimental and observation groups scored significantly lower with BI than those in the control group ( P 0.05). ConclusionUSN had significant impact on the recovery of the independent living of stroke patients. The rehabilitative intervention for USN may improve ADL performance of stroke patients with USN.