Objective To study the relationship between the expression of inflammatory factors and joint function in patients with traumatic arthritis and the expression of inflammatory factors in joint fluid.Methods 90 cases of knee joint trauma in our hospital for the treatment of traumatic arthritis patients from September 2015 to September 2016 as the object of study,90 healthy volunteers as control group,two groups were detected in the synovial fluid of patients with inflammatory factors,to explore the level of inflammatory factors in synovial fluid (TNF-α,IL-6,IL-8) in patients with X-ray classification and joint function.Results TNF-α,IL-6 and IL-8 levels in the synovial fluid of patients with traumatic arthritis were significantly higher than that in healthy adults(P<0.05),radiographic evidence of traumatic osteoarthritis in patients with grade Ⅰ 37 cases,Ⅱ 23 cases,Ⅲ grade 21 cases,9 cases of grade Ⅳ,with bone damage aggravated statistically significant TNF-α,IL-6,IL-8 level is high and the differences between groups,patients with HSS score and KSS score in patients with joint X-ray classification,TNF-α and IL-6 in synovial fluid and IL-8 level is negatively related to(P<0.05).Conclusion The expression of inflammatory factors and joint function in patients with traumatic arthritis were closely related to the expression of inflammatory factors.

Apoptotic mechanism of Pathogenic fungi has become a focus of study due to the worsening situation of clinical drug resistance in fungi. Apoptosis of Pathogenic fungi is a complicated network involving many pathways, regulators and effectors. Besides, it is closely related to the autophagy, mitochondrial apoptotic pathways, and changes of endogenous and exogenous factors. As a new target of antifungal agent, apoptotic pathways are of great clinical importance. This paper reviews the apoptotic pathways of Pathogenic fungi.

Objective To study the changes of plasma brain natriuretic peptide(BNP)level in patients with atrial fibrillation(AF),and explore the relationship between plasma level of BNP and paroxysmal AF,persistent AF,rheumatic AF and non-rheumatic AF.Methods In total,158 outpatients and inpatients diagnosed as heart diseases were selected from the Affiliated Hospital of Inner Mongolia Medical College during October 2005 to January 2007,83 with AF and 75 without AF.Their cardiac function Was evaluated according to the classification system of the New York Heart Association(NYHA)in the United States.Plasma level of BNP was measured with bi-monoclonal antibody microparticulate enzyme immunoassay(MEIA).Results Plasma BNP level was significantly higher in AF group[(112±75)ng/L]than that in non-AF group[(39±26)ng/L,P＜0.05]with same ventricular function.No significant difference in plasma level of BNP between the groups with paroxysmal AF[(113±84)ng/L]and with persistent AF[(110±69)ng/L]was found.Plasma level of BNP Was(84±73)ng/L in the group with rheumatic valvular AF,not significantly different from that in non-valvular AF[(117±76)ng/L,P＞0.05].Conclusion Plasma BNP level is higher in AF group than that in non-AF group,but no significant difference in plasma BNP level between patients with paroxysmal AF,persistent AF,rheumatic valvular AF and non-rheumatic AF,under same ventricular function,was found.

Objective To investigate the expression levels of forkhead transcription factor 3 (FOXP3) and cytotoxic T lymphocyte-associated antigen-4(CTLA-4) in the peripheral blood of children with newly diagnosed type 1 diabetes mellitus(T1DM),and explore their roles in the pathogenesis of T1DM. Methods Fifty children with newly diagnosed T1DM and 30 healthy children( control group) were select-ed. FOXP3 and CTLA-4 mRNA expressions were detected using real-time PCR. Soluble FOXP3 ( sFOXP3 ) and CTLA-4(sCTLA-4) protein levels in the serum were assayed by ELISA,and the levels of diabetes anti-bodies,HbA1C and C-peptide were determined using Western blotting,high performance liquid ion-exchange chromatography,and electrochemiluminescence, respectively. Results The expressions of FOXP3 mRNA and protein in T1DM patients were lower than those in the control group[0. 95 ± 0. 48 vs. 2. 11 ± 0. 79,(6. 27 ± 1. 49) ng/ml vs. (9. 02 ± 2. 37) ng/ml,respectively,both P <0. 01],while the expressions of CTLA-4 mRNA and protein were higher than those in the control group[2. 43 ± 0. 83 vs. 1. 94 ± 0. 84,(77. 88 ± 22. 34) ng/ml vs. (65. 97 ± 12. 11) ng/ml,respectively,both P<0. 01]. In T1DM patients,the expressions of FOXP3 and CTLA-4 genes presented positive correlation with the protein expression( r=0. 758,0. 396, P<0. 05), and FOXP3 protein expression presented correlation with CTLA-4 protein expression ( r =-0. 624,P<0. 05). Conclusion There are abnormal expressions of FOXP3 and CTLA-4 genes as well as their proteins in the peripheral blood of the children with newly diagnosed T1DM,and FOXP3 regulated the expression of CTLA-4 in T regulatory cells,indicating the involvement of immune mechanisms in the devel-opment of T1DM.

Objective To investigate pregnancy complications,cesarean delivery and abnormal fetal presentation in women with uterine anomalies.Methods Fifty women diagnosed with uterine malformation and sixty women with normal uterus were retrospectively analyzed .Results The group comprised women with uterine malformation had significantly higher stillbirth or fetal death (P <0.05) and lower rates of the full-term delivery(P <0.05) than that of the control group.The subgroup comprised women with uterine anomalies had significantly higher rates of cesare -an section(P <0.05) and abnormal fetal position(P <0.05) than the control group.The birth weight and length of infant in observation group were significantly lower than those in control group (P <0.05).The rate of placenta abnormalities in uterine anomalies was significantly higher than that in control group (P <0.05).The incidences of premature rupture of membrane,cord entanglement,oligohydramnios,postpartum hemorrhage and neonatal asphyxia in observation group were no significant difference than those in control group .The rate of premature rupture of membrane(PROM) in uterus unicornis was significantly higher than that in other types (P <0.05).Conclusion Compared with women with normal uterus ,the incidences of abnormal fetal position ,the placenta abnormalities,pre-mature delivery,and stillbirth or fetal death in women with congenital uterine anomalies are high .Uterine anomalies are associated with a decrease in the birth weight and length .We should pay more attention to finding the uterine malformation and treatment before pregnancy .Antenatal care should be reinforced in pregnancy women with uterine anomalies.

Objective To observe the effect of the joint treatment containing single sialic acid four hexose gangliosides,clopidogrel,atorvastatin in the treatment of cerebral infarction.Methods 88 acute cerebral infarction patients met the inclusion criteria were randomly divided into the observation group of 44 cases and the control group of 44 cases.The control group were given clopidogrel and atorvastatin,and the observation group were give single sialic acid four hexose gangliosides on the base of the control group,100mg/time,qd,4 week for a course.The symptoms before and after treatment were observed.The NIHSS and ADL score were calculated.Blood lipids,CRP,IMT was inspected.Results The total effective rate of the observation group was 90.91％,the control group was 77.27％,the observation group was significantly higher than the control group (x2 =11.967,P ＜ 0.05) ; the TC,TG,LDL-C,HDL-C,CRP,IMT,ADL,NIHSS of both group after treatment were significantly improved than before treatment,there were a statistically significant differences (all P ＜ 0.05).After treatment,the TC,TG,LDL-C,HDL-C,CRP,IMT,ADL NIHSS of observation group and the group were (4.06/ ± 0.98) mmol/L vs (4.18 t 1.02) mmol/L,(1.35 ±0.73)mmol/L vs (1.42 ±0.81)mmol/L,(1.68 ±0.82) mmol/L vs (1.74 ±0.85)mmol/L,(1.44 ±0.58) mmol/L vs (1.41 ±0.52)mmol/L,(40.55 ±3.12)mg/L vs (47.27 ±3.57)mg/L,(0.78 ±0.08)mm vs (0.87 ±0.09)mm,(28.9 ± 11.3) scores vs (22.3 ± 10.0) scores,(8.2 ± 5.3) scores vs (15.5 ± 6.2) scores.the CRP,IMT,ADL,NIHSS of observation group were improved more significantly than the control group,the differences were statistically significant(all P ＜ 0.05).Conclusion Jointing treatment containing single sialic acid four hexose gangliosides,clopidogrel,atorvastatin can improve significantly lipid levels,inhibit platelet aggregation,make a stability of carotid atherosclerotic plaque,improve activities of daily living and neurological deficit,but also can prevent secondary brain damage after traumatic brain injury,which is the ideal solution for the treatment of cerebral infarction.

Objective: To observed the efects of estrogen and wall-shear stress alone or in combination on the proliferation and function of rat osteoblast in vitro. Methods: Isolated and purified osteoblast from the calvaria of newborn SD rats were cultured and passaged.The cells of passes three to four were treated with 0.1 nmol/L estrogen (goup E),wall-shear stress at 80 r/min (group WSS) or 0.1 nmol/L combined with wall-sher stress at 80 r/min (group EWSS) respectively.Cell proliferation was studied by MTT assay and alkline phosphatase (ALP ) by a ALP test kit. Results: Both the estrogen and wall-shear stress alone caused the increase of cells proliferation and alkaline phosphatase (ALP) activity.But long period of wall-shear stress decreased the cell proliferation.Wall-shear stress increased ALP activity more quickly and more remarkably than the estrogen did.The combination of estrogen and wall-shear stress increased the cell proliferation and the ALP activity.In the early stage (6～24 h) of the cell proliferation, the combination functioned synergicly.The combination functioned antagonistically on ALP from 6 to 12 h while synergicly after 12 h. Conclusion: Estrogen and wall-shear stress can elevate the cell proliferation, ALP activity of osteoblasts in vitro.

Objective To investigate the efficacy of intracytoplasmic sperm injection (ICSI) combined with percutaneous testicular sperm aspiration (PTSA)in the treatment of severe male infertility. Methods From October 1998 to December 2000, 162 couples were enrolled, and ICSI and PTSA were adopted. Ovarian stimulation was achieved by the short protocol. All metaphase Ⅱ (MⅡ) oocytes were selected for ICSI. Results 1 517 MⅡ oocytes were injected in 185 cycles, 990 fertilized (65.3%), and 152 embryo implanted. 54 couples achieved clinical pregnancy (35.5%). Conclusion PTSA combined with ICSI is a rapid, convenient, painless and effective approach for the treatment of severe male infertility.

To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups: control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.

Objective To evaluate the role of intercellular gap junction in lung injury induced by hemorrhagic shock and resuscitation in rabbits. Methods Twenty-four healthy rabbits of both sexes weighing 1.5-2.2 kg were used in this study. The animals were anesthetized with iv 3% pentobarbital 30 mg/kg,tracheostomized and mechanically ventilated. Femoral artery was cannulated for MAP monitoring, blood-letting.Right external jugular vein was cannulated for fluid infusion. Hemorrhagic shock was induced according to the method described by Wiggers. MAP was maintained at 35-40 mm Hg for 60 min. The animals were then randomly divided into 2 groups (n = 12 each):traditional treatment group (group A) and octanol group (group B). Both groups received rapid iv infusion of lactated Ringer's solution (LR solution) 1.5 ml·kg-1 ·min-1 for 30 min (T3 ).In group B 99.5% octanol (a specific gap junction inhibitor) 5 mmol/kg was injected intraperitoneally (IP) in addition to iv LR solution infusion. Thirty minutes later the animals were resuscitated with infusion of the blood withdrawn and LR solution (the volume was equal to the volume of blood loss). Then LR solution was infused iv at 2.5 ml· kg-1 · h-1 for 150 min ( T4 ). Left ventricular systolic pressure (LVSP) and HR were recorded before blood letting (T1), immediately after successful establishment of the model (T2), and at T3 and T4. The mortality during resuscitation was calculated. The animals were killed at T4. Blood samples were taken from femoral artery for determination of the serum protein concentration. The lungs were removed. The left lower lobe was lavnged. The protein concentration in broncho-alveolar lavage fluid (BALF) was measured. Lung permeability index (LPI) was calculated (LPI = protein concentration in BALF protein concentration in serum). Na+-K+-ATPase and Ca2+-ATPase activities and W/D lung weight ratio were determined and microscopic examination was performed. Results HR was significantly lower at T3 and T4 and LVSP higher at T4 in group B than in group A. The pulmonary permeability index, W/D lung weight ratio and mortality were significantly lower and the activities of Ns+-K+-ATPase and Ca2+ -ATPase were significantly higher in group B than in group A. The lung tissue danage was significantly ameliorated in group B. Conclusion Intercellular gap junction is involved in the development of lung injury induced by hemorrhagic shock and resuscitation in rabbits.