Objective To investigate the relationship between renal function and lipid metabolism in patients with chronic renal disease .Methods A total of 76 patients with chronic renal disease were enrolled and classified into chronic renal failure(CRF)group (29 cases) ,chronic nephritis(CN)group(21 cases) ,diabetic nephropathy (DN) group(14 cases) and nephrotic syndrome (NS) group (12 cases) ,and 65 healthy subjects were enrolled in control group ,the relationships between renal function ,lipid metabolism , hypersensitive C-reactive protein (hs-CRP) and 24 hours urinary albumin excretion rate (UAER) were analyzed .Results Urea ni-trogen(Urea) ,serum Cystatin C(CysC) ,cretinine(Cr) ,uric acid(UA) ,β2-microglobulin(β2-MG) ,hs-CRP ,low density lipoprotein (LDL-c) .Lp(a) .24h UAER were significantly higher in CN group;Urea ,Cr ,β2-MG ,CysC ,hs-CRP ,ApoA ,lipoprotein A[Lp(a)] , 24 h UAER were significant higher in DN group;Urea ,hs-CRP ,TC ,TG ,HDL-c ,LDL-c ,ApoB ,Lp(a) ,24h UAER were significant-ly highly in NS group;Urea ,Cr ,UA ,β2-MG ,CysC ,hs-CRP ,TG ,LDL-c ,Apolipoprotein b(ApoB) ,Lp(a) ,24 h UAER were signifi-cantly higher in CRF group .Conclusion With the changes of renal function in patients with chronic renal diease ,the lipid metabo-lism and hs-CRP also change .

Objective To study the effect of binocular vision function training for fusion in the eye position and three levels of eye function in adult patients after concomitant strabismus surgery. Methods 56 cases with postoperative concomitant strabismus were randomly divided into training group and control group . In the training group, patients were trained with software to enhance the fusion. After training for 7, 30, 180 and 365 days, the eye position, the fusion and the stereoesthesia were observed and analyzed. Results After training for 365 days, the deteriorative ratio in the training group is 3%, which is significantly lower than control group (23%)(P<0.05). The correction ratio in eye position is 20%, which is significantly higher than control group (4%)(P<0.05). The recovery ratio in binocular vision function is 53%, which is significantly higher than control group (15%)(P<0.05). The recovery ratio in stereaeuity is 46%, which is significantly higher than control group (11%)(P<0.05). Conclusions Fusion training can enhance the recovery of fusion and stereaeuity, decrease the deteriorative ratio, and correct the eye position after surgery in concomitant strabismus patients.

Under the environment of network,the service mode of subject librarian becomes an important strategy of intelligence serves in university libraries.To provide reference for other librarians,this paper summarizes services provided for the academic researchers by using Web of Knowledge platform resources.

ATP-tumor chemosensitivity assay(ATP-TCA)is a tumor chemosensitivity assay in vitro.Beacause of the special merites of A11P-TCA,it is seemed to be the most promising and effective method of tumor chemosensitivity in vitro.Application of ATP-TCA in clinic has got significant effects.ATP-TCA is a good method for individualized chemotherapy.

Objective:To investigate the difference of biological characteristics and responses to PDGF-BB between human ovarian carcinoma-derived microvascular endothelial cells and human umbilical vein endothelial cells.Methods:The ovarian carcinoma-derived microvascular endothelial cells were isolated and purifed from ovarian carcinoma tissues.Compared to the human umbilical vein endothelial cells,the biological characteristics and responses to PDGF-BB were observed in OdMEC for the changes of growth,PCNA and VEGF expression.Results:20ng/ml PDGF-BB could enhance both kinds of endothelial cells to proliferate,express PCNA and VEGF.The proliferation and PCNA protein expression in HUVEC were augmented more significantly than those in OdMEC,the protein and mRNA of VEGF expression were augmented more significantly than those in HUVEC.Conclusion:The responses of OdMEC to PDGF-BB were significantly different from those of HUVEC,which provides experiment basis for research on ovarian carcinoma vascularization and antiangiogenesis therapy.

The important role of library in reading popularization was elaborated, with the main ways for reading popularization and the principal information literary education methods for reading popularization librarians listed, including learning of professional knowledge and skills, training of career virtue, and reading brand popularization.The training of reading popularization librarians and selection of characteristic brand plans can provide the theoretical basis for library to carry out this innovative service.

Objective To explore the factors influencing health management demand by community residents.Methods Using data from the survey of Health Management Demand by Community Residents in Hangzhou,this paper examined the influence factors with the logistic model from such four aspects as individual characteristics,economic characteristics,health level and health awareness.Results The health awareness poses a positive impact on health management demand;the level of health has a negative impact while income has an inflection point(5 000 yuan per month).However,individual characteristics have no significant effect. Conclusion Such measures as enhancing health management awareness of the residents,building a health management system centering on communities and supported by health management firms,and building health management information platform,can effectively satisfy such demand.

Objective To understand the prevalence and awareness of Toxoplasma gondii of the pregnant women in Bazhou City,Hebei Province,so as to provide the evidence for formulating effective prevention and control interventions. Methods The pregnant women with prenatal screening in the Fourth People’s Hospital in Langfang City were selected and investigated with questionnaires. A part of the pregnant women were randomly sampled and received the ELISA test of the antibodies against T. gondii. Results Totally 672 pregnant women accepted the questionnaire survey,with an awareness rate of 19.49%(131/672). Totally 526 pregnant women of them were randomly selected to take the ELISA test,with a positive rate of 13.88%(73/526). The awareness rate and infection rate of T. gondii were related to the education level,occupation and place of residence (c2=5.89-17.62,all P<0.05),and the infection rate was negatively related to the awareness rate(c2=5.37,P<0.05). Con?clusion Increasing the awareness rate of toxoplasmosis knowledge is one of the effective methods to control the disease. There?fore,the health education about T. gondii should be carried out actively.

Objective To prepare mouse anti-human monoclonal antibodies against B7-H6 and to identify their biological characteristics. Methods The B7-H6 gene was cloned by RT-PCR from a human lung adenocarcinoma cell line ( A549 ) and then subcloned into the eukaryote expression vector pCMV3 to construct the recombinant vector pCMV3-B7-H6. The recombinant vector pCMV3-B7-H6 that was verified with enzyme digestion and gene sequencing was transfected into NIH/3T3 cells by electroporation. BALB/c mice were immunized with the successfully transfected cells named 2H8 through intraperitoneal injection. The monoclonal antibodies against human B7-H6 with the advantages of high affinity and specificity were pre-pared by using hybridoma technology. Western blot assay and flow cytometry analysis were used to identify the specificity of prepared monoclonal antibodies. Results The recombinant eukaryotic expression vector encoding B7-H6 was successfully constructed. Two hybridoma clones that stably secreted monoclonal anti-bodies against B7-H6 were screened out by using flow cytometry analysis and the monoclonal antibodies se-creted by them were belonged to IgG2a isotype. Specific reactions between B7-H6 and the secreted mono-clonal antibodies were confirmed by Western blot assay and flow cytometry analysis. Conclusion The mon-oclonal antibodies which recognized B7-H6 specifically were prepared successfully.

Objective To investigate the perioperative nursing demands of elderly patients receiving laparoscopic cholecystectomy ( LC) . Methods A total of 195 elderly patients receiving LC were interviewed .The nursing care demands at admission, preoperation, postoperation, and discharge were collected respectively . Results On the day of admission, all the 195 patients (100%) wished to be aware of their surgeons , 191 patients ( 97.9%) wished to understand causes of disease and its prevention, 189 patients (96.9%) were willing to know the operation costs , and 124 patients (63.6%) wanted to know the influences of basic diseases on operation.During preoperative periods, 194 patients (98.5%) expected to understand the safety and time of operation, 187 patients (95.9%) were interested in the preparedness before operation , and 135 patients (69.2%) wanted to know about the strategy for treating operation stress .During postoperative periods , 195 patients ( 100%) wanted to know the operation process and outcomes , 185 patients ( 94.9%) wanted necessary cares from nurses , 172 patients ( 88.2%) wanted to know the management for postoperative pain , 148 patients ( 75.9%) expected to understand the drug prescription after operation , and 146 patients (74.9%) were interested in diets and physical activities .At the time of discharge, 195 patients (100%) wanted to be aware of reexamination time and follow-up context, 193 patients (99.0%) wanted to know dietary recommendation , and 177 patients (90.8%) were willing to know the exercise options and time . Conclusion Nurses should be aware of the perioperative care demands in elderly patients receiving LC and provide specific care services to reduce complications and improve rehabilitation .