1.Effects of Qiwei Qianliening Tablets on nonbacterial chronic prostatitis and its anti-inflammatory and analgesia activity
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: To investigate the effects of Qiwei Qianliening Tablets(QLN)(Radix paeoniae rubra,Semen Vaccariae,Semen Citri reticulatae,etc) on nonbacterial chronic prostatitis and its anti-inflammatory and analgesia activity. METHODS: Nonbacterial chronic prostatitis model was induced by injecting 25% Xiao Zhiling Injection into the prostate of rat and then coefficent of prostate and pathologic changes were observed.Anti-inflammatory effects were tested by cotton pelle-induced granuloma in rat.Analgesia activity was tested by hot-plate method. RESULTS: QLN inhibited the increase of prostate tissue weight and the histopathology showed resolving prostatitis in QLN-treated groups compared with the controls.Anti-inflammatory activity was confirmed in cotton pelle-induced granoluma in rat and QLN-treated groups extended the licking-paw latency in hot-plate method compared with the controls. CONCLUSION: Qiwei Qianliening Tablets has prevention and treatment effect on experimental nonbacterial chronic prostatitis.
3.The application of podcast in nursing education in China and abroad
Shaoying DU ; Ying WANG ; Haoying DOU
Chinese Journal of Practical Nursing 2014;30(11):5-7
Podcast,a versatile web-based technology,has been widely employed in international nursing education.In China,however,it is only applied based on its video-related function.The other functions,such as subscribing,participating,mobile,and sharing learning option have not been fully exploited.The studies on application of podcast demonstrate that it can excite the learning interest,alleviate stress and anxiety,and enhance the preclinical educational experience.Moreover,it facilitates the self-study ability.Therefore the podcast is expected to be applied more widely in the domestic nursing education.
4.Toll-like receptors expression and function of human metapnemnovirus infected human lung epithelial cells
Ying DOU ; Xiaodong ZHAO ; Yao ZHAO
Chinese Journal of Microbiology and Immunology 2009;29(12):1122-1127
Objective To investigate the expression changes of Toll-like receptors(TLR) and the signaling pathway function on airway epithelial cells infected with human metapneumovirus(hMPV), and to explore the mechanisms of hMPV-induced airway inflammation. Methods A549 cells were infected with hMPV live or co-cultured with UV-inactivated hMPV in vitro. Growth curve of the virus in A549 cells was established and mRNA expression of TLR were detected by RT-PCR assay and real-time PCR assay at 3, 6, 9, 12, 24 h post infection. IFN-α and TNF-α in the culture supernatants were detected by ELISA. Results hMPV was able to replicate in A549 cell, growing to peak titer of 10~(5.2) TCID_(50)/ml at 3 d after infection. Most of TLR mRNA levels were up-regulated by hMPV infection but not UV-inactivated viruses via RT-PCR assay (P<0.05) after 6 h of stimulation. Real-time PCR assay showed that TLR3-4, TLR7-9 mRNA increased after being infected by hMPV in a time-dependent manner. The expression of IFN-α and TNF-α in the cul-ture supernatants were significantly up-regulated after 24 h infected by hMPV. Conclusion hMPV infection up-regulates the expression of TLR in lung epithelial cells. The inflammatory response to hMPV may associ-ate with part of TLR signal transduction pathway.
5.Relationship between Quantity of Alcohol Intake and Blood Pressure
Xianyan JIANG ; Xiangfeng DOU ; Ying YANG
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(02):-
Objective To explore the association between the daily intake of alcohol and blood pressure in Chinese.Methods With the baseline data of the Qingdao Port Health Study,all subjects aged 18 to 54 yrs including all in-service and retired employees in Qingdao port were selected and divided into seven groups according to their daily intake of alcohol: groups of 0,0~,5~,10~,20~,40~ and 60~g alcohol/d,also stratified by ages.The amount of alcohol intake was assessed by a questionnaire.The cross-sectional association between blood pressure and intakes of alcohol was analyzed with general linear model and Logistic regression.Results In the groups of 25~34,35~44,45~54 yrs old both before and after adjustment,systolic and diastolic blood pressure were linearly increased with the amount of alcohol intake from the 0 g/d group to 60~g/d group.In the group of 18~24 yrs old,there was no obvious relationship between BP and alcohol intake.Logistic regression model showed that odds ratios in the groups of 0~g/d,5~g/d,10~g/d,20~g/d,40~g/d and 60~g/d versus the abstainers was 1.05,1.14,1.08,1.24,1.30 and 2.02,respectively,and the prevalence of hypertension in the 20~g/d group was significantly higher than that in the 0 g/d group and groups of low or moderate alcohol intake.Conclusion An increasing and linear relationship between alcohol consumption and blood pressure was established,and alcohol intake ≥20 g /d is an independent risk factor for hypertension.
6.Mechanism studies underlying the alleviatory effects of isoliquiritigenin on abnormal glucolipid metabolism triggered by type 2 diabetes
Zi-yi CHEN ; Xiao-xue YANG ; Wen-wen DING ; Dou-dou WANG ; Ping HE ; Ying LIU
Acta Pharmaceutica Sinica 2024;58(1):105-118
Isoliquiritigenin (ISL) is an active chalcone compound isolated from licorice. It possesses anti-inflammatory and anti-oxidative activities. In our previous study, we uncovered a great potential of ISL in treatment of type 2 diabetes mellitus (T2DM). Therefore, this study aims to reveal the mechanism underlying the alleviatory effects of ISL on T2DM-induced glycolipid metabolism disorder. High-fat-high-sugar diet (HFD) combined with intraperitoneal injection of streptozotocin (STZ) were used to establish T2DM mice model. All animal experiments were carried out with approval of the Committee of Ethics at Beijing University of Chinese Medicine. HepG2 cells were used in
7.Isoliquiritigenin alleviates energy metabolism imbalance in type 2 diabetic mice
Wen-wen DING ; Xiao-xue YANG ; Zi-yi CHEN ; Dou-dou WANG ; Ping HE ; Ying LIU
Acta Pharmaceutica Sinica 2023;57(11):3339-3348
Isoliquiritigenin (ISL) is a flavonoid compound isolated from licorice. It possesses excellent antioxidant and anti-diabetic activities. This study aims to investigate the molecular mechanism underlying the alleviatory effect of ISL on energy metabolism imbalance caused by type 2 diabetes mellitus (T2DM). 8-week-old male C57BL/6J mice were used in
8.Establishment of mouse model for human metapneumovirus infection
Ying DOU ; Yao ZHAO ; Zhiyong ZHANG ; Xiaodong ZHAO
Chinese Journal of Microbiology and Immunology 2009;29(4):312-315
Objective To establish a mouse model for human metapneumovirus (hMPV) infec-tion, and to explore the course of viral replication and pathology and lay ground for vaccine development. Methods BALB/c mice were infected intranasally with hMPV, and sacrificed on day 1,3, 5, 7, 9 and 16 post inoculation. Lungs were used for viral isolation, viral titration by an improved plaque assays, pulmonary histopathology and detection of hMPV mRNA expression by RT-PCR. Results Live viruses were successful-ly isolated from the lungs of infected mice, hMPV replicate well in the lungs and viral titers peaked on the 5th day post inoculation (5.16 ± 1.09)×105 PFU/g, remaining to be detectable on the 9th day post inocu- lation (2.79 ± 1.22)×102 PFU/g. Genomic RNA of hMPV was able to be detected on the 16th day post in-oculation by RT-PCR targeting F gene. Histopathology of lungs was characterized by interstitial pneumonia in particular during 3 ~ 7 days post inoculation. Conclusion hMPV infected BALB/c mice model was success-fully established, hMPV infected mice showed similar patterns of viral replication and pulmonary histopathol-ogy as in infected humans.
9.The effect of stent implantation on the quality of sexual activity in middle and old age male patients with exertional angina pectoris
Zhe YAN ; Ying DOU ; Mingtai WANG ; Fucheng SUN
Chinese Journal of Postgraduates of Medicine 2013;36(28):20-22
Objective To explore the effect and mechanism of stent implantation on the quality of sexual activity in middle and old age male patients with exertional angina pectoris.Methods One hundred and seventy-two male patients with exertional angina pectoris were enrolled in this study,and they were divided into control group (94 patients) and percutaneous coronary intervention group (78 patients).The patients in control group were given routine pharmacotherapy and the patients in percutaneous coronary intervention group were given drug-eluting stent implantation and routine pharmacotherapy.The sexual activity function was evaluated by international index of erectile function (IIEF)-5 scale before treatment and 3 months after treatment.The times of sexual intercourse was added up.The evaluation of angina pectoris was performed by the grade of Canadian cardiovascular society (CCS).At the same time,the walking distance and heart rate were measured through 6-minute walking test.The left ventricular ejection fraction(LVEF) was detected by echocardiogram.Results After treatment for 3 months,the scores of IIEF-5,the degree of sexual satisfaction,the times of sexual intercourse and the grade of CCS in percutaneous coronary intervention group were significantly improved compared with those in control group [(20.58 ± 7.36) scores vs.(16.38 ± 6.35) scores,(4.32 ± 1.38) scores vs.(2.51 ± 1.89) scores,(6.3 ± 3.2) times/month vs.(3.5 ± 1.6) times/month,(1.10 ± 0.43) grades vs.(2.50 ± 1.1 0) grades] (P < 0.05).The walking distance and heart rate after 6-minute walking test and LVEF in percutaneous coronary intervention group were significant improved compared with those in control group [(386 ± 82) m vs.(281 ± 86) m,(95 ± 18) times/minute vs.(122 ± 25) times/minute,(65.2 ± 11.2)% vs.(55.6 ± 13.8)%] (P < 0.05).Conclusions Stent implantation can significandy improve the quality of sexual activity in middle and old age male patients with exertional angina pectoris.The mechanisms are associated with the exercise capacity increasing and the frequency of angina pectoris attacks decreasing during sexual intercourse.
10.Effect of lansoprazole and rabeprazole on dual-antiplatelet therapy in patients with coronary heart disease
Zhe YAN ; Ying DOU ; Mingtai WANG ; Fucheng SUN
Chinese Journal of Postgraduates of Medicine 2013;36(31):7-9
Objective To investigate the influence of proton pump inhibitors (lansoprazole and rabeprazole) on dual-antiplatelet therapy (DAPT) in patients with coronary heart disease.Methods One hundred and eighty-one cases of coronary heart disease with DAPT were selected,and they were divided into control group (66 cases,no proton pump inhibitors administered),lansoprazole group (65 cases,lansoprazole administered) and rabeprazole group (50 cases,rabeprazole administered).100 mg/day of aspirin and 75 mg/day of clopidogrel were concomitantly used in all patients.Platelet aggregation rate,platelet aggregation threshold index (PATI) and adenosine diphosphate (ADP) was measured.Results The ADP-PATI in control group was (3.47 ± 0.96) μ mol/L,lansoprazole group was (3.28 ± 1.05) μ mol/L,rabeprazole group was (3.32 ±0.83) μ mol/L,and there was no statistically significant difference among three groups (P>0.05).There was also no statistically significant difference in platelet aggregation rate and collagen-PATI among three groups (P > 0.05).Conclusion In patients with coronary artery disease,the concomitant use of lansoprazole or rabeprazole does not affect antiplatelet action during DAPT.