Objective:To study the influence of Taurine on proliferation of neural stem cells after focal cerebral ischemia in rats and to explore the mechanism of taurine in treating ischemic brain injury.Methods:The model of middle cerebral artery occlusion was made by using opening ranium hot coagulation in 80 rats,who were divided randomly into normal group,sham operate group,model group and taurine group.Taurine was administrated once daily by i.p.in taurine group,and immunohistochemistry method was used to observe the number of Brdu positive cells on the 3rd,7th,14th and 21st day after ischemia.Results:There were Brdu positive cells in the cortex,hippocampal dentate gyrus(DG) and striatum at different time points after cerebral ischemia,and the number of Brdu positive cells in various brain regions increased significantly on the 7th day as(18.65 ?4.38),(25.63?3.58),(19.46 ?2.98) and having statistical significance(P

AIM: To observe the difference in the application of proportion of pinellisa decoction for purging stomach-fire, ginger rind and licorice root with different BP (back propagation,BP) method of artificial neural network. METHODS: The influence of gastric mucuqas content in normal rats was measured, and the groups of different flavor of a drug and dosage were divided according to homogeneous design. This research was programmed with MATLAB6.5, conformed the experimental data with BP artificial neural network, and the research compared the different structural topology of 8-3-1?8-8-1?8-12-1, and compared the different influence with the different BP method to network. And then, the expected mode of gastric mucus content was formed, which based on the BP neural network. RESULTS: The artificial neural network model that had 8-10-1 structural topology and improved BP method was formed, and it was a kind of good method to uniform the studied samples and to forecast the unstudied samples, Moreover, the expected effect of variable learning rate back propagation was best. CONCLUSION: Using the studying of pinellisa decoction for purging stomach-fire, ginger rind and licorice as an example, and give birth to the new model in studying proportioning of compound prescription. This model will found its position in studying proportioning of compound prescription.

Pulse shape and pulse force are difficult to detect in pulse taking study. But the application of visualized technology extends the space acquisition of pulse taking information, and it is possible to realize the objective detection of pulse shape and pulse force. Rational research thoughts and strategies could be informed by combining image information and other data, and it is a necessary method in implementing the objective detection of pulse.

AIM: To explore the relationship between berberine content of Banxiaxiexin Decoction in rat serum and gastric motility. METHODS:Berberine content of Banxiaxiexin Decoction was determined by HPLC; Gastric residue was observed in all the rats with a marker of dextran blue 2000 to determine the capacity of gastric evacuation. RESULTS: Banxiaxiexin Decoction markly promoted gastric evacuation ( P

AIM: To investigate the contractile effect of berberine on antral smooth muscle cells in rat stomach. METHODS: Smooth muscle cells were isolated from the antrum of the SD rat stomach. Different doses of berberine were added into cell suspension. Contractibility of smooth muscle cells were measured by micrometry. RESULTS: Compared with the control group the length of the treated cells(0.5g?L -1 ,1g?L -1 ) was significantly short( P

The paper discussed a variety of experimental designs of compound compatibility law of traditional Chinese medicine (TCM): study of whole formula and different ingredients of formula. The latter includes study of single ingredient, study of functional ingredient group, orthogonal design, clustering analysis, homogeneous design, factorial analysis and so on. It was proposed that experimental designs of formula should be based on the theory of TCM, and combined with modern sciences.

Objective To study the mechanism of Hezhong Jiangni Formnla(HJF) in protecting esophageal mucosa.Methods Rat models of reflux esophagitis were established by cardioplasty,pyloric ligation and roux-Y type gastrojejunostomy to evaluate the degree of esophageal mucosa injury.After treatment of HJF,the gastric juice was collected with the method of pyloric ligation to determine its volume and acidity and the activity of pepsin.The gastrointestinal motility was observed after oral use of charcoal powder.The level of malondialydehyde(MDA)in esophageal mucosa was measured to evaluate the esophageal antioxidative ability.Results HJF relieved the injury of esophageal mucosa dose-dependently;it had no obvious influence on the volume and acidity of gastric juice and the activity of pepsin,but it could obviously improve the gastrointestinal motility.MDA level of esophageal mucosa in the model group was increased significantly as compared with the control group.Pretreatment with HJF could greatly inhibited the formation of MDA.Conclusion HJF has protective effect on esophageal mucosa,which may be related to the improvement of gastrointestinal motility and its antioxidant action.

Objective: To observe the protective effects of Gastrodine on the cultivated rat brain microvessel endothelial cells damage by mimic cerebral ischemia.Methods: The endothelial cells activity,survival rate,the change in NO content,and the effects of Gastrodine were observed in the cultivated rat brain microvessel endothelial cells(BMEC) damaged by mimic cerebral ischemia.Results: The activity and survival rate of BMEC in the ischemia groups are obviously lower than that in the normal groups;compared with the normal groups,the activity,survival rate and NO content of BMEC in the Gastrodin groups have the increasing tendency;comparing to the ischemia groups,the activity of BMEC in the Gastrodin groups obviously increasing(P

Objective:To study the relationship between different pulse forces and cyclical power(CP)of radial artery motion.Methods:39 patients’and 20 healthy persons’ ECG and pressure sphygmograms were collected synchronously with NX-1 sphygmograph.On the basis of automatic detecting R waves of ECG and FFT analysis of pressure sphygmograms with SPTool of MATLAB 7.0.1,change in frequency domain of radial artery motion with different forces was observed and CP was worked out.Results: There is signifi cant deviation between CP of pulse tracings with different forces(pulse tracings with powerful force〉pulse tracings with normal force〉pulse tracings with weak force).Conclusion: CP of radial artery motion can be used to discriminate pulse force.

Objective:To establish experimental teaching model for slippery pulse by mini-pig.Methods:The model of slippery pulse was established by driping low molecule dextran in vein.The normal and slippery pulse were extracted from axil artery of mini-pig by two experienced traditional Chinese physician through double blind method.Meanwile,the correlative parameters of pulse graph of axil artery such as MSAB,MSBC,HFF,HE/HB and TW were extracted through optimizational extraction method by using NX-8 multifunctional sphygmograph.Results:The pulse rate of slippery pulse of mini-pig was slightly fast than that of normal pulse.The rhythm of slippery pulse was regularity,the nger sensation was powerful,and the pulse syate was smooth.Compared with the normal pluse,the pulse graph of slippery pluse displayed a steep ascend ramus,high and narrow B wave,tiny D wave,lower E valley and obvious F wave.HB,MSAB and MSBC increased(P