1.EXPRESSION AND SIGNIFICANCE OF SIALYL-LeX IN COLORECTAL CARCINOMA
Peizhong SHANG ; Huaping GU ; Yinchen SUN
Chinese Journal of Bases and Clinics in General Surgery 2001;8(3):151-153
Objective To investigate the level of cell adhesion molecule sialyl-LeX expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis. Methods Sialyl-LeX expression and its optical density in colorectal carcinoma (n=90) and remote normal mucosa (n=30) were quantitatively studied with microwave-LSAB immunohistochemical method combined with image analysis technique. Fifty-three patients were followed up. Results The weaker staining in remote normal colorectal mucosa was observed in very limited parts of some deep crypts. Positive rate of sialyl-LeX expression was only 16.7%(5/30). The positive expression of sialyl-LeX was observed in 83 of 90 patients with colorectal carcinoma(92.2%). The apical cytoplasma of cancer tubules, the luminal contents, and the cytoplasma of the cancer cells were strongly stained. The mean integral optical density of sialyl-LeX positive cell in poorly differentiated adenocarcinoma was significantly higher than that in highly differentiated and mucinous ones (P<0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones (P<0.01). With followed-up for longer than 5 years, it was much lower in the alive cases than that in the dead (P<0.01). Conclusion These findings indicate that changes of sialyl-LeX expression and its optical density is related to carcinogenesis, differentiation, invasion and metastasis of colorectal carcinoma. It may be a good predicter for the prognosis of patients with colorectal carcinoma.
2.Expression of LeX ,Sialyl-LeX, LeA and Sialyl-LeA in Colorectal Carcinoma and Its Significance
Peizhong SHANG ; Huaping GU ; Yinchen SUN
Journal of Chinese Physician 2000;0(11):-
Objective To investigate the level of cell adhesion molecules LeX , sialyl-LeX, LeA and sialyl-LeA expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis.Methods The expression forms of LeX,sialyl-LeX,LeA and sialyl-LeA were studied by immunohistochemistry method with catalysis signal amplification (CSA)in 90 carcinomas and 30 normal mucosal specimens of colon and rectum, as well as in 51 metastatic lymph nodes.Fifty-three patients were followed up. Results Their antigens were expressed in normal mucosa distant from carcinoma lesions with the following frequencies: LeX, 93 3% (28/30); sialyl-LeX, 16 7% (5/30); LeA, 93 3% (28/30); and sialyl-LeA, 90 0% (27/30), whereas in colorectal carcinomas, the expression frequency rate were: LeX, 90 0% (81/90); sialyl-LeX, 94 4% (85/90); LeA, 78 9% (71/90); and sialyl-LeA, 80 0%(72/90). In metastatic lesion of lymphode LeX, sialyl-LeX, LeA and sialyl-LeA were expressed in 68 7% (35/51),96 1% (49/51),66 7% (34/51) and 66 7% (34/51), respectively.Conclusions The alteration of Lewis-related carbohydrate antigens in cancer cell membranes, including sialylation and/or aberrant glycosylation,these alteration may be related to metastatic behavior. These results suggested that sialyl-LeX may be used for estimating infiltration, metastasis and prognosis as a tumor-associated antigen in colorectal carcinoma.
3.Study of the Antigen Retrieval By Microwave and Expression of CD_(44)s and CD_(44v6) Genes Protein in Human Intracranial Metastastic Tumors and Glioblastomas
Zhengwen XIONG ; Yinchen SUN ; Hong SU
Journal of Chinese Physician 2001;0(01):-
Objective To investigate the effect of antigen retrieval by microwave and the expression of CD 44 s and CD 44v6 in human intracranial metastastic tumor and human glioblastomas, and the relationship between the expression of CD 44 adhesion molecules and the invasion or metastasis of these tumors.Methods After antigen retrieval by microwave-0.1%Triton X-100(MW-T) and buffer solution of microwave-citrate salts(MW-CA), CD 44 s and CD 44v6 were used to detect the expression of CD 44 adhesion molecules in 20 normal human brain tissues, 35 human glioblastomas, and 30 intracranial metastastic tumor by microwave-LSAB immunohistochemical staining technique.Results Integral optical density(IOD) value of CD 44 s and CD 44v6 positive cells restored by MW-T and MW-CA was higher significantly than that of without antigen retrieval (P
4.A study on the expression of sialyl Lewis X in colorectal carcinoma
Peizhong SHANG ; Huaping GU ; Yinchen SUN
Chinese Journal of General Surgery 2001;0(10):-
Objective To investigate the expression and distribution of sialyl Lewis X in colorectal carcinoma and its relationship with carcinogenesis, progression and metastatic proclivity.Methods Microwave LSAB immunohistochemical technique was used to detect the expression of sialyl Lewis X in colorectal carcinoma and in normal mucosa. Immunoelectromicroscopic localization of sialyl Lewis X labelled by colloidal gold was also observed.Results The positive rate of sialyl Lewis X expression in primary colorectal cancer was 92 2%(83/90), and 16 7%(5/30) in normal mucosa. The positive rate was 100% in patients with lymphatic nodes metastasis, compared with that of negative nodes of 82 1% ( P
5.Herniography in the diagnosis of occult or recurrent inguinal indirect hernia
Peizhong SHANG ; Zhenhai ZHANG ; Yinchen SUN ; Jingzhang LIU ; Dengkai LIU
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo improve the diagnosis of occult or recurrent inguinal indirect hernias. Methods 47 patients with obscure groin or incisional pain with normal physical examinations were undergone herniography. Results26 patients had hernias confirmed by herniography, including 22 indirect and 4 recurrent hernias. The symptoms disappeared after herniorrhaphy in the 26 patients. Eighteen of the other 21 patients with normal herniography were asymptomatic via other treatment 3 months after herniography. Conclusions Herniography is useful to diagnose the occult or recurrent inguinal indirect hernias in patients with inguinal region pain of unclear causes.
6.Clinical approach to the neuroischemic ulcers in diabetic foot
Jinan CHEN ; Yinchen CHEN ; Xinjuan SUN ; Wei WANG ; Ning LUO ; Jie ZHANG ; Aiping WANG
Chinese Journal of Endocrinology and Metabolism 2015;(9):819-823
[Summary] Neuroischemic diabetic foot ulcer ( NDFU) is characterized by infection, ulceration of deep tissues, neurological abnormalities, and various degrees of peripheral vascular disease in the lower limbs. The patients often have multiple risk factors such as older, longer duration, cardiovascular disease. The treatment is very difficult. The prognosis depends on the severity of complications, tissue range of infections, and the peripheral vascular disease. In this article, the treatment process of an old inpatient with NDFU and severe complications was reviewed and to propose a standard pathway for its management.
7.Application of 64-Row Volumetric CT Three Dimensional Image Formation in Skull Neoplasty
Jinguo YUAN ; Zhiming WANG ; Zangzhu CAO ; Zhenmei TAN ; Bin FENG ; Shusheng HAN ; Yinchen SUN
Chinese Journal of Rehabilitation Theory and Practice 2009;15(7):681-682
Objective To assess the application of 64-row volumetric CT three dimensional image formation to shape the titanium mesh for the skull neoplasty. Methods 40 cases were divided into the shaping before operation group (21 cases), in which the titanium meshes were shaped with the data from 64-row volumetric CT skull three dimensional image formation before operation; and the shaping during operation group (19 cases), in which the titanium meshes were shaped approximately before and exactly during operation. The time of shaping, anaesthesia and the whole operating were compared, and the qualities of the skull plasty were assessed. Results All the skull repaired satisfactorily. The time of shaping, anaesthesia and the whole operating was shorter in shaping before operation group than in shaping during operation group (P<0.05). No complication was observed. Conclusion Application of 64-row volumetric CT three dimensional image formation in the skull neoplasty may reduce the surgery time and risk.
8.Uncoupling protein 2 variants and cell proliferation and apoptosis of human umbilical vein endothelial cells
Yinchen SHEN ; Feng'e CHEN ; Tao SUN ; Qing GU ; Kun LIU ; Zhi ZHENG ; Yihui CHEN ; Ning WANG ; Xun XU
Chinese Journal of Ocular Fundus Diseases 2017;33(1):52-56
Objective To observe the influences of uncoupling protein 2 (UCP-2) rs660339 variants transfection on cell proliferation and apoptosis of human umbilical vein endothelial cell (HUVEC). Methods Two UCP-2 green fluorescent protein (GFP) lentivirus constructs were created with the rs660339 locus carried C or T (UCP-2C or UCP-2T), respectively. HUVEC were cultured after lentiviral infection of UCP-2C or UCP-2T. The expression of UCP-2C or UCP-2T was detected with real time polymerase chain reaction. Cell proliferation and cell apoptosis were compared among negative control (NC) group, UCP-2T group and UCP-2C group using CCK-8 cell viability and flow cytometry. Western blot and immunostaining were employed to examine the expression of Bcl-2 gene. Results The lentivirus constructs were successfully created.>80%of the transfected cells were found to express GFP under fluorescent microscope. The mRNA levels of UCP-2 gene were significantly increased (F=29.183, P=0.001) in the UCP-2T group and UCP-2C group. The CCK-8 assay revealed that on day two (F=15.970, P=0.004), day three (F=16.738, P=0.004), day four (F=5.414, P=0.045) post-infection, UCP-2T and UCP-2C group showed significantly greater proliferation than the NC cells. The apoptotic rate in the UCP-2T and UCP-2C group was significantly lower than NC group (F=277.138, P=0.000), and the apoptotic rate of UCP-2T was significantly lower than that of UCP-2C (P=0.003). The protein levels of Bcl-2 in the UCP-2T and UCP-2C group were significantly greater than that in the NC group (F=425.679, P=0.000), and the Bcl-2 expression of UCP-2T was greater than that of UCP-2C (P=0.002). The Bcl-2 density in the UCP-2T and UCP-2C group were greater than that in the NC group (F=11.827, P=0.008), while there was no difference between UCP-2T and UCP-2C group (P=0.404). Conclusion The variants of UCP-2 rs660339 may influence HUVEC proliferation and apoptosis, and UCP-2T showed a stronger effect of inhibiting apoptosis than UCP-2C.
9.64 Row Volumetric CT 3D Reconstruction in Severe Craniocerebral Injury
Jinguo YUAN ; Bin FENG ; Caozhu CAO ; Shusheng HAN ; Yinchen SUN ; Fuzeng LIU ; Hongyu ZHU ; Liyuan LUO ; Zhibo LI
Chinese Journal of Rehabilitation Theory and Practice 2011;17(8):717-718
Objective To explore 64 row volumetric CT 3D reconstruction in the clinical application of severe craniocerebral injury.Methods 60 patients with craniocerebral injury in our hospital were retrospectively analyzed who accept craniocerebral 64 row volumetric CT 6 to 24 hours after injury. Results All cases got timely diagnosis and treatment, especially for the special parts of brain lesions, ventricle base of skull fractures and pool, and various angles show. Conclusion 64 row volumetric CT reconstruction for the diagnosis of craniocerebral injury shows important significance and may estimate the prognosis in diagnosis, guiding treatment and surgery, reducing misdiagnosis.
10.Interpretation and comparison of guidelines for diagnosis and treatment of diabetic foot between International Working Group on Diabetic Foot and Chinese Diabetes Society
Hui YANG ; Xinjuan SUN ; Zhiwei HU ; Yinchen CHEN ; Aiping WANG
Chinese Journal of Endocrinology and Metabolism 2020;36(3):201-206
This exploratory study attempted to establish the rules in diagnosis and treatment of diabetic foot, through interpretation and comparison of the guidelines for diabetic foot from domestic with international ones. The document provided comparison between Guidelines of International Working Group on Diabetic Foot (IWGDF) and Chinese Diabetes Society (CDS) in 2019, related to diabetic foot disease on: methodology, content, prevention, offloading, peripheral artery disease (PAD), infection, wound healing interventions, and classification of diabetic foot ulcers. Prevention of ulcers in persons with diabetes foot is very important, and a non-removable offloading device is the first-choice of offloading treatment; Surgical indications and reasonable treatment should be mastered in PAD; Different anti-infection treatments, including surgical debridement, should be used base on the severity of foot infection; There are lots of treatments to improve healing, however the Grand Standard of medical evidence is not very high; There are a larger number of proposed classifications and scoring systems for diabetic foot, but none of them could cover all the needs of diagnosis and treatment. Thus, the principles outlined have to be adapted or modified by our health care professionals, based on local circumstances, to develop a standardizated procedure in diagnosis and treatment of diabetic foot.