Objective To investigate the modified herniorrhaphy method for adult oblique inguinal hernia. Methods From June 2001 to June 2005,the clinical data of 56 paitients with oblique inguinal her-nias were analyzed retrospectively. Results Rapid recovery,with few complication as well as low cost were achieved.Follow-up time was in 6 months to 5 years.Postoperative recurrence was not found. Conclusion With modified herniorrhaphy,the normal anatomical structure of internal ring were restored and the trans-verse fascia defect are strengthened at the same time. Normal anatomical structure of inguinal canal are main-tained. This procedure possesses a higher cure rate and postoperative recurrent rate may be reduced obviously.

The precocious puberty in female rats were induced by subcutaneous injection of N-methyl-DL-aspartate (NMA). The female rats with induced precocious puberty showed the same sexual feature as the rats that had normal puberty. NMA plays its role at the level of hypothalamus. Subcutaneous injection of NMA is an effective means to induce true precocious puberty in female rats.

60 mm Hg and PaCO2

Objective　The aim of this paper was to investigate endothelial dysfunction，platelet activation, and inflammation in elderly hypertensive patients and those complicated with cerebral infarction. Methods　Twenty-eight elderly hypertensive patients complicated with cerebral infarction(within 72 hours after onset of neurological symptoms),thirty-one Stage Ⅰ～Ⅱelderly hypertensive patients and twenty-eight elderly healthy individuals were selected as subjects.Their plasma concentrations of von Willebrand factor(vWF), alpha granule membrane protein-140(GMP-140), and serum concentrations of C-reaction protein(CRP) were examined. Results　Elderly hypertensive patients complicated with cerebral infarction had significantly higher plasma vWF, GMP-140, and serum CRP than elderly hypertensive patients and elderly healthy individuals. Plasma vWF and GMP-140 were higher in elderly hypertensive patients than elderly healthy individuals, whereas serum CRP was increased slightly and there was no significant difference between elderly hypertensive patients and elderly healthy individuals. Conclusion　This study suggests that elderly hypertensive patients complicated with cerebral infarction have obvious endothelial dysfunction, platelet activation, and inflammatory change. Stage Ⅰ～Ⅱ elderly hypertensive patients have obvious endothelial dysfunction and platelet activation as well.

OBJECTIVE To explore the incidence rate,pathogen distribution and characteristics of drug resistance of ventilator-associated pneumonia(VAP) patients due to acute lung injury and acute respiratory distress syndrome(ALI/ARDS) after severe injury,and analyze impact factors on VAP.METHODS All 183 cases with ALI/ARDS after severe injury from Jan 2004 to Mar 2008,were admiitted and given mechanic ventilation over 48 hours in our ICU and EICU.RESULTS VAP was found in 98 cases,accounted for 53.56%.Among them,42 were early-onset VAP and the other 56 were late-onset VAP.Altogether 276 pathogens were isolated,and most of them were Pseudomonas aeruginosa,Staphylococci aureus,Klebsiella pneumoniae,Acinetobacter baumannii and Escherichia coli.In 41 cases only one pathogen was isolated,the other 57 cases were with two or above pathogens.The revealed important risk factors were coma,prolonged mechanical ventilation,tracheotomy,antiacid treatment,use of antimicrobial agents and low albumin.CONCLUSIONS The incidence rate of VAP due to ALI/ARDS is high,and most of the isolated pathogens are drug resistant.Mixed infections are found commonly in these cases.

Objective To establish the preparation techniques for preparing the reference substance of platycodin-D.Methods Raw material of platycodi radix was extracted with 70 %MeOH.The concentrated extract passed through D101 macroporous resin column,then the 40 %alcohol eluate was separated by silica gel column chromatography with chloroform-methanol gradient elution.The fractions contained platycodin-D were collected and purified by HPLC.Results HPLC analysis and normalization of peak areas showed the purity of the platycodin-D was 98.8 %.Conclusions The preparation techniques are simple and high yield,can be used for preparing the reference substance of platycodin-D.

Object To study the causes for the occurrence of red skin and dark green skin of Panax quinquefolius L. during processing and its mechanism. Methods To observe the phenomena and analyze the result which simulated the processing condition of P. quinquefolius based on its components. Results The cross section of P. quinquefolius began to appear light red at 40 ℃, lasting 72 h. Whereas the time of browning was shortened with the temperature rising. The cross section of P. quinquefolius turned green while dipping in solution of Fe 3+ ion of 0 01 mol/L for 25 min. The time of turning green was shortened with the increasing concentration of Fe 2+ , Fe 3+ solution. Conclusion The results show that red skin of P. quinquefolius was caused by the Maillard reaction while drying at the excessive higher temperature. Whereas the complex reaction between phenolic substances in P. quinquefolius and metal ions during processing might result in dark green skin of P. quinquefolius. This expounds the mechanism of red skin and dark green skin turning during the P. quinquefolius processing in the theory.

Objective To develop a set of indicators for measuring the performance of China's Healthcare Improvement Initiative.Methods Such methods as literature review,expert consultation and onsite preliminary experiment were used,to study and demonstrate the indicators,the evaluation framework,the weight,evaluation indicators,and evaluation standards.Results The final evaluation indicators for medical institutions included nine class-1 indicators,29 class-2 indicators,and 56 class-3 indicators;those for healthcare administrations included six class-1 indicators,nine class-2 indicators,and 13 class-3 indicators.Conclusions The system takes into full account the special nature of healthcare,and mission of the Initiative,as the well as the quality,safety,efficiency and equity of healthcare,to make the system scientific and operational.

Objective:To investigate the effect and mechanism of transcription factor Foxp 3 on the proliferation and cell cycle of human lung adenocarcinoma cell line A 549.Methods:We knocked down the expression of Foxp 3 using siRNA.Foxp3 inhibition was detected by RT-PCR.Cell proliferation was detected by MTT.Cell cycle of A549 cells were detected by flow cytometry after the transfection of siRNA.Cell cycle-related checkpoint genes were filtered by RT-PCR.The regulation of Foxp3 on cell cycle-related checkpoint genes were detected by immunofluorescence and dual -luciferase reporter assay system.Results: The proliferation of A549 cells were inhibited after silencing Foxp3,and A549 cells were arrested in G0/G1 cycle.G1/S cycle checkpoint gene CCND1 was down regulated.Mechanism research show that Foxp 3 can regulate the expression of CCND 1 directly.Conclusion: Foxp3 can promote the proliferation of A549 cell line by up regulating G 1/S cycle checkpoint gene CCND 1.This provides a new target for the therapeutic targets of lung adenocarcinoma.