1.Improving teaching effects of medical image by exploring the teaching strategy
Bingqi SHEN ; Liqing XIAN ; Zhongjiao YIN ; Jianping CHU
Chinese Journal of Medical Education Research 2006;0(07):-
The purpose of the present study was to investigate teacher-dominant action and focus on learning-oriented group.Medical teachers should pay more attention to three important aspects,such as optimizing teaching pattern,dealing with the relationship carefully among pre-class,inter-class and post-class.It is important to attach importance to pre-class,giving prominence to inter-class and also giving attention to post-class,which can form a harmonious,interactive and collaborate class.In addition,it stimulates deep processing interactions between teachers and students by increasing students learning interests,which is very helpful in improving teaching effects of medical image.
2.Amine-lyases and Their Applications in Preparation of Pharmaceutical Intermediates
Bi-Bo HE ; Xiao-Long CHEN ; Yu-Guo ZHENG ; Yin-Chu SHEN ;
Microbiology 2008;0(07):-
Carbon-nitrogen lyases (E.C.4.3) are a group of enzymes that release ammonia, amidine or amino group etc, and also show ability to form double bond or ring structure. Specifically, enzymes forming amino group are called amine-lyases (E.C.4.3.3), which are critical in the industrial production of many medicine intermediates. In this review is a summary of four major amine-lyases in terms of their source, enzymatic characteristics and their applications in preparation of pharmaceutical intermediates.
3.Biosynthesis of Tautomycin and Its Inhibition Region of Protein Phosphatase
Xiao-Tao CHAI ; Xiao-Long CHEN ; Yu-Guo ZHENG ; Yin-Chu SHEN ;
Microbiology 2008;0(10):-
Tautomycin is one of well-known specific protein phosphatase inhibitors and exhibiting potent antifungal ability, especially to Sclerotinia sclerotiolum. This article reviews the recent research progress of tautomycin, focusing on its inhibition region and biosynthesis.
4.BREEDING OF PHAFFL4 RHODOZYMA FOR ASTAXANTHIN OVER-PRODUCTION
Pu WANG ; Juan-Ping QIU ; Yu-Guo ZHENG ; Yin-Chu SHEN ;
Microbiology 1992;0(01):-
Phaffia rhodozyma is a good strain for astaxanthin production. An over-producing mutant YB-20-29 was obtained by means of Cs137-?ray and N-methy1-N'-nitro-N-nitrosoguanidin (NTG) treatment. The biomass for this strain by shake culture was 36.32 g/L, the pigment content was 1216.0 ?g/g, an increase of 308% compare to o-riginal strain. The astaxanthin content in broth was 30.9?g /mL. It was a potential strain for astaxanthin over-production.
5.Experimental study of the bioresorbable collagen membrane used for guided bone regeneration around dental implants.
Wei FEI ; Xiao-min YANG ; Zheng LI ; Ming-ping YIN ; Zhi-hao SHEN ; Chu-hang LIAO
West China Journal of Stomatology 2008;26(5):494-498
OBJECTIVETo investigate the effects of using guided bone regeneration (GBR) technique with an bioresorbable collagen membrane for repairing bone defects around dental implants.
METHODSIn 12 mongrel dogs, pure titanium implants were immediately implanted in mandibles after extraction of the mandibular third and fourth premolars of both sides, in which standard bone defects of 3 mm x 3 mm x 5 mm were created at mesial side of the implants. The defects on right side were covered with Co membrane, the left side was uncovered as control. The dogs were sacrificed 1, 2, 4, 6 months postoperatively. The specimens were removed and studied by gross observation, X-ray radiograph, histological examination, scanning electron microscope (SEM) and pull-out test.
RESULTSThe quantity and quality of new bone formation in experimental side which bone defects covered with Co membrane were much better than that in the control side.
CONCLUSIONWith the biodegradable property and excellent biocompatibility, Co membrane can be used for guided bone regeneration to promote the bone repair progress, and the promotion acts mainly at early stage of bone healing.
Animals ; Bone Regeneration ; Collagen ; Dental Implantation, Endosseous ; Dental Implants ; Dogs ; Guided Tissue Regeneration, Periodontal ; Mandible ; Membranes, Artificial ; Titanium
6.Development of Dipolar Proton Transfer Reaction Mass Spectrometer for Real-time Monitoring of Volatile Organic Compounds in Ambient Air
Qiang-Ling ZHANG ; Xue ZOU ; Qu LIANG ; Ya-Ting ZHANG ; Ming-Jian YI ; Hong-Mei WANG ; Chao-Qun HUANG ; Cheng-Yin SHEN ; Yan-Nan CHU
Chinese Journal of Analytical Chemistry 2018;46(4):471-478
Volatile organic compounds (VOCs) in ambient air can participate in photochemical reactions, which lead to the generation of secondary pollutants such as ozone and aerosol. So real-time and accurate monitoring of atmospheric VOCs plays an important role in the study of the causes of air pollution. On the basis of proton transfer reaction mass spectrometry (PTR-MS) research, a novel dipolar proton transfer reaction mass spectrometer (DP-PTR-MS) for real-time and on-line monitoring atmospheric VOCs was developed. Compared with the conventional PTR-MS with one kind of reagent ion H3O+, DP-PTR-MS had three kinds of reagent ions H3O+, OH-, (CH)2COH+, which could be switched according to the actual detection need. So DP-PTR-MS can improve the qualitative ability and expand the detection range effectively. The reagent ion H3O+can be used for detecting VOCs whose proton affinities are greater than that of H2O. The reagent ion OH-can be used to identify VOCs cooperating with the reagent ion H3O+,and can also be used for detecting some inorganic substances such as CO2. The reagent ion (CH3)2COH+can be used for accurately detecting NH3under interference elimination circumstances. The limit of detection (LOD) and sensitivity of DP-PTR-MS were measured by using six kinds of standard gases. The results showed that the LOD for detecting toluene was 7×10-12(V/V) and the sensitivity for detecting ammonia has reached 126 cps/10-9 (V/V). The ambient air in Hefei city was on-line and real-time monitored for continuous 78 hours with DP-PTR-MS. The results showed that the newly developed DP-PTR-MS could be used for long-term and real-time monitoring atmospheric VOCs with the concentration of 10-12(V/V) level. DP-PTR-MS is an important tool for the study of the causes of atmospheric pollution and the monitoring of trace VOCs emissions.
7.Value of cerebral small vessel disease burden in predicting prognosis after endovascular therapy for acute ischemic stroke
Gao MA ; Zixin YIN ; Xiaoquan XU ; Shanshan LU ; Guangchen SHEN ; Yue CHU ; Sheng LIU ; Haibin SHI ; Feiyun WU
Chinese Journal of Radiology 2024;58(1):41-47
Objective:To assess the value of cerebral small vessel disease (CSVD) burden in predicting prognosis in acute ischemic stroke (AIS) patients with anterior circulation large vessel occlusion (LVO) after endovascular therapy (EVT).Methods:The study was a cross-sectional study. A total of 242 patients with AIS due to anterior circulation LVO received EVT in the First Affiliated Hospital of Nanjing Medical University from February 2018 to September 2022. The clinical and imaging data of all patients were analyzed retrospectively. On follow-up MRI within 7 days after EVT, CSVD features [white matter hyperintensity (WMH), lacune, perivascular space, cerebral microbleed, cerebral atrophy] and CSVD burden score (0-5) was evaluated. Modified Rankin scale (mRS) score at 90 days after EVT was assessed. Patients were categorized into a mild burden group (0-1 points) and a moderate-severe burden group (2-5 points) based on CSVD burden score. Meanwhile, patients were categorized into a good prognosis group (0-2 points) and a bad prognosis group (3-6 points) based on mRS score at 90 days after EVT. Mann-Whitney U test and χ2 test were used to compare the difference of clinical and imaging indexes between the 2 groups, and variables with P<0.1 in the univariate analysis were included in the multifactorial logistic regression to screen for independent factors to predict the prognosis. Results:There were 169 patients in the good prognosis group and 73 patients in the bad prognosis group out of 242 patients. Compared with the good prognosis group, age, incidence of hyperlipidemia, baseline National Institutes of Health Stroke Scale (NIHSS) scores, incidence of hemorrhagic conversion, CSVD burden scores, incidence of periventricular WMH scores of 3 and/or deep WMH scores≥2, and incidence of moderate-severe cerebral atrophy of patients in the bad prognosis group were higher, and the incidence of complete recanalization was lower (all P<0.05). Multivariate analysis showed hyperlipemia ( OR=8.438, 95% CI 1.691-42.119, P=0.009), baseline NIHSS score ( OR=1.103, 95% CI 1.047-1.162, P<0.001), complete recanalization ( OR=0.131, 95% CI 0.038-0.454, P=0.001) and hemorrhage transformation ( OR=1.952, 95% CI 1.031-3.697, P=0.040) were independent factors for the prognosis of EVT in patients with LVO AIS. There were 157 cases in the mild burden group and 85 cases in the moderate-severe burden group. The 90-day mRS score was higher in the moderate-severe burden group compared with the mild burden group ( Z=-2.24, P=0.025). Conclusion:CSVD burden has some clinical implications in predicting the prognosis of EVT in patients with anterior circulation LVO AIS.
8. Multicenter epidemiological investigation of hospitalized elderly, young and middle-aged patients with severe burn
Yong TANG ; Liangxi WANG ; Weiguo XIE ; Chuan′an SHEN ; Guanghua GUO ; Junjie CHEN ; Chunmao HAN ; Licheng REN ; Zhigang CHU ; Meifang YIN ; Yuan WANG ; Dongxia ZHANG ; Yuesheng HUANG ; Jiaping ZHANG
Chinese Journal of Burns 2017;33(9):537-544
Objective:
To compare and analyze the epidemiological characteristics of hospitalized elderly, young and middle-aged patients with severe burn in recent years, so as to provide reference for the prevention and treatment of elderly patients with severe burn.
Methods:
Relying on the entry system of epidemiological case data and biological sample of severe burn from multicenter in clinic, medical records of patients with severe burn, aged above 18, hospitalized in 8 burn wards from January 2012 to December 2015 were collected. Six hundred and fifteen patients who were more than 18 years old and less than or equal to 65 years old were included in young and middle-aged group (YM). Eighty-two patients aged more than 65 years old were included in elderly group (E). Data of age, gender, residence, education level, cause of injury, location of injury, season of injury, total burn area, occurrence and area of full-thickness burn injury, wound site, inhalation injury incidence and severity, post burn admission time, proportion of delayed resuscitation, proportion of escharectomy or tangential excision and skin grafting, preinjury systemic disease, system complication during hospitalization, length of hospital stay, outcome of treatment, and reason of abandoning treatment of patients were analyzed. Data were processed with chi-square test and Mann-Whitney
9.Extracorporeal shock wave therapy in cubital tunnel syndrome: A pilot study
Yu-Ping Shen ; Yin-Yin Wu ; Heng-Yi Chu ; Tsung-Ying Li ; Liang-Cheng Chen ; Yung-Tsan Wu
Neurology Asia 2018;23(3):233-238
To investigate the clinical therapeutic effect of extracorporeal shock wave therapy in the
treatment of cubital tunnel syndrome. Methods: Seven patients (10 elbows) with moderate cubital
tunnel syndrome participated in this study. Three sessions of radial extracorporeal shock wave therapy
(2,000 shots, 4 Bar, 5 Hz) (once a week) were administered to the ulnar nerve at the proximal cubital
tunnel region. The primary and secondary outcomes were assessed using the Visual Analog Scale
(VAS) and the shortened Disabilities of the Arm, Shoulder and Hand questionnaire (Quick DASH),
respectively, at the 4th, 8th, and 12th week, following the 3rd session of shock wave therapy. Results:
The VAS and Quick DASH scores demonstrated improvements at all follow-up time points, in all
treated elbows. The mean VAS and Quick DASH score improved from 4.7±0.3(mean±SE) to2.2±0.2
and 16.6±2.1to 6.8±1.6 respectively during 12 weeks follow up (all p <0.01).
Conclusion: This pilot study revealed the safety and efficacy of extracorporeal shock wave therapy
in patients with moderate cubital tunnel syndrome.
10.Clinical guidelines for the treatment of ankylosing spondylitis combined with lower cervical fracture in adults (version 2024)
Qingde WANG ; Yuan HE ; Bohua CHEN ; Tongwei CHU ; Jinpeng DU ; Jian DONG ; Haoyu FENG ; Shunwu FAN ; Shiqing FENG ; Yanzheng GAO ; Zhong GUAN ; Hua GUO ; Yong HAI ; Lijun HE ; Dianming JIANG ; Jianyuan JIANG ; Bin LIN ; Bin LIU ; Baoge LIU ; Chunde LI ; Fang LI ; Feng LI ; Guohua LYU ; Li LI ; Qi LIAO ; Weishi LI ; Xiaoguang LIU ; Hongjian LIU ; Yong LIU ; Zhongjun LIU ; Shibao LU ; Yong QIU ; Limin RONG ; Yong SHEN ; Huiyong SHEN ; Jun SHU ; Yueming SONG ; Tiansheng SUN ; Yan WANG ; Zhe WANG ; Zheng WANG ; Hong XIA ; Guoyong YIN ; Jinglong YAN ; Wen YUAN ; Zhaoming YE ; Jie ZHAO ; Jianguo ZHANG ; Yue ZHU ; Yingjie ZHOU ; Zhongmin ZHANG ; Wei MEI ; Dingjun HAO ; Baorong HE
Chinese Journal of Trauma 2024;40(2):97-106
Ankylosing spondylitis (AS) combined with lower cervical fracture is often categorized into unstable fracture, with a high incidence of neurological injury and a high rate of disability and morbidity. As factors such as shoulder occlusion may affect the accuracy of X-ray imaging diagnosis, it is often easily misdiagnosed at the primary diagnosis. Non-operative treatment has complications such as bone nonunion and the possibility of secondary neurological damage, while the timing, access and choice of surgical treatment are still controversial. Currently, there are no clinical practice guidelines for the treatment of AS combined with lower cervical fracture with or without dislocation. To this end, the Spinal Trauma Group of Orthopedics Branch of Chinese Medical Doctor Association organized experts to formulate Clinical guidelines for the treatment of ankylosing spondylitis combined with lower cervical fracture in adults ( version 2024) in accordance with the principles of evidence-based medicine, scientificity and practicality, in which 11 recommendations were put forward in terms of the diagnosis, imaging evaluation, typing and treatment, etc, to provide guidance for the diagnosis and treatment of AS combined with lower cervical fracture.