Objective To study the influence of Clopidogrel combined Aspirin to treat cerebral artery stenosis of young people and P-selectin in blood plasma. Methods 142 cases were randomly divided into therapy group (70 cases) and control group (72 cases). The two groups were given Aspirin ( 0. 1/d, Bayer company) by peroral administration or nasal feeding( deglutation disturbance) and simultaneously given tatin drug, Venoruton injection, Citicoline and Salvia Miltiorrhiza injection. The therapy group were added clopidogrel(75mg/d,Shenzhen Salubris Pharmaceuticals Co, Ltd). Venous blood was taken suction to detect platelet packing fraction, P-selectin and Doppler ultra-sound and carotid artery blood flow velocity before medication,24 hours after medication ,7 days after medication. Results Platelet packing fraction, P-selectin were significantiy decreased in the two groups than before treatment( P <0. 05)and therapy group were sigrificantly lower than control group( P < 0. 01 ). The control group after 7 days of treatment, transcranial Doppler ultrasound and carotid artery blood flow velocity significantly improved ( P < 0. 05 ), but the treatment group more significantly (P < 0. 01 ). Conclusion Blood plate was activated in patients of cerebral artery stenosis of young people, Clopidogrel combined with Aspirin could improve the blood rheology and hemodynamics, reduce the volume of cerebral lesions and improve the prognosis of patients.

With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.
Bioengineering ; Industrial Microbiology ; Pichia

Objective:To investigate the effect of porcelain bake cycles on oxide layer form ed on cobalt-chromium alloy surface.Methods:Fixed spot analysis of EPMA methods was adopted to measure the oxygen at o mic percentage inward from the edge of cross-section interface of alloy pieces. The adherent state of oxide layer on the surface of substrate alloy was observed .Results:The oxide layer on the surface of alloy had stable thickness and contain ed more and more oxide as porcelain firing times increased.In some area,oxide la yer fractured from the substrate metal.Conclusion:Cobalt-chromium alloy frame should be abrased and polished thoroughl y after porcelain bake cycles.

About the malignant tumor in China,the hepatocellular carcinoma mortality is second only to lung cancer and serious threat to the life and health of the masses.Furthermore,because most patients has been in advanced cancer during medical treatment,so had lost the chance of one-stage surgical resection.However,the sensitivity of hepatocellular carcinoma to chemotherapy,radiotherapy and other treatments are poor.Transcatheter arterial chemoembolization is the main method of the treatment for patients those have lost the chance of operation,though the clinical effect is significant,the inadequate is also presence,such as tumor necrosis,incompletely clear,residual tumor nidus and the damage of the immune function after operation.Recombinant adenovirus p53 gene can validly infect tumor cells,transcription and expression of p53 protein,it also regulate the expression of related genes inhibiting tumor cell growth and induce cell apoptosis directly or indirectly.It is increasingly highly attention that recombincanting rAd-p53 with transcatheter arterial chemoembolization(TACE) to treat hepatocellular carcinoma.In order to evaluate the clinical value and promising future,we will make a brief summary for the research progress in this area in recent years.

Through the simulation based on a two-dimensional whole-heart electrophysiological model, the effect of ventricular electrical activity on the precordial cardioelectrical field as well as the ECG manifestation have been discussed. Such effect can vary with the changed excitation sequence of ventricles and the changed active and passive electrical properties of cardiac tissues. As the result, many abnormal ECG waves can form. By carefully analyzing such effect, the correlation among ECG waveforms recorded at different lead locations can be revealed and the significance of ECG manifestation can be fully explored.As a case of analyzing, some ECG waves at certain right chest lead locations can get a proper explanation.

G-protein-coupled receptors (GPCRs) are major targets for drug discovery. The regulator of G-protein signaling (RGS) proteins is a recently identified protein family,which strongly modulates the activity of G proteins. RGS proteins accelerate the deactivation of G proteins to reduce GPCR signaling; however, some also have an effector function and transmit signals. Combining GPCR agonists with RGS inhibitors should potentiate responses and markedly increase the regional specificity of agonist. In the central nervous system, RGS proteins have unique tissue distributions and are strongly regulated by signal transduction events. Thus the diversity of RGS makes them attractive targets for pharmacotherapy of neurological disorders.

Objective To study the effectiveness of long-range electrocardio-monitoring system for aged people outside the hospital.Methods Long-range electrocardio-monitoring network system was adopted to perform monitoring 3 times every day for 320 retired leaders in Tianjin.Results The coincidence rate was 92.4%,and the morbidity rate 1.1%.Conclusion The long-range electrocardio monitoring network system can transfer patient's real-time information.Patients can be monitored by hospital at any time and in any place.

Objective To observe effect of personalized health education on drug compliance and efficacy in patients with multidrug-resistant pulmonary tuberculosis. Methods From October 2015 to May 2016, 100 cases with multidrug-resistant tuberculosis were selected. The patients were randomly divided into two groups, 50 cases in each group. The contorl group was given a routine clinical health education, the observation group were received personalized health education . Awareness of disease and medication, medication compliance and satisfaction after education in the two groups were compared. Results The total awareness rate in the observation group was 96.00% (48/50), the total medication compliance rate was 94.00% (47/50), the satisfaction degree was (87.62±6.95) points. The above three indicators in the observation group were better than those in the control group (P<0.05). Conclusion It is possible to improve the patient's awareness and adherence to disease and medication after taking the personalized health education program in patients with multidrug-resistant tuberculosis disease clinically. It can get a better satisfaction test result.

Penile vascular endothelial damage, vasomotor dysfunction, and blood flow deficiency are the major causes of erectile dysfunction (ED). Current management of ED mostly depends on selective phosphodiesterase type 5- (PDE5) inhibitors, which fail for some ED patients. For Peyronie's disease-induced ED, surgical and physical therapies are used in addition to PDE5I medication, but frequently it is difficult to achieve satisfactory results. Recent studies show that the low-intensity extracorporeal shockwave therapy can promote angiogenesis and improve blood flow to the penis, which promises to be a novel effective therapy for ED and Peyronie's disease.
Erectile Dysfunction ; therapy ; High-Energy Shock Waves ; therapeutic use ; Humans ; Male ; Penile Induration ; therapy

Objective To investigate the prevalence of metabolic syndrome (MS) among residents in Shuimogou district of Urumqi city and to compare the differences between the Uygur and Han Populations. Methods Adopting cluster sampling, a survey of diabetes mellitus was conducted in Shuimogou district in June, 2008. Data of total 2420 subjects aged over 18 years with complete data, including background information of each individual, blood pressure, lipid profile, blood glucose and so on, were analyzed in this study. The criterion of the China Diabetes society (CDS) in 2004 was applied for diagnosis. Results The crude prevalence rates of MS were 12.85% in total, 14.91% in male and 7.39% in female, respectively. The age-standardized prevalence rates of MS were 9.93% in total, 13.58% in male and 6.11% in female. The prevalence rates of MS in male was higher than that in female (P0.05). The prevalence rates of components of MS were 43.31% for obesity, 30.08% for hypertension, 25.79% for hypertriglyceridemia and 16.28 % for hyperglycosemia. Among the four factors above, there were about 65% subjects who had at lest one abnormal factor according to the CDS criterion. Conclusion There were no differences in the prevalence rates of MS between the Uygur and Han populations. However, the prevalence rate of MS in male was higher than that in female. More than half subjects had at least one abnormal metabolic factor.