Objective To observe effect of personalized health education on drug compliance and efficacy in patients with multidrug-resistant pulmonary tuberculosis. Methods From October 2015 to May 2016, 100 cases with multidrug-resistant tuberculosis were selected. The patients were randomly divided into two groups, 50 cases in each group. The contorl group was given a routine clinical health education, the observation group were received personalized health education . Awareness of disease and medication, medication compliance and satisfaction after education in the two groups were compared. Results The total awareness rate in the observation group was 96.00% (48/50), the total medication compliance rate was 94.00% (47/50), the satisfaction degree was (87.62±6.95) points. The above three indicators in the observation group were better than those in the control group (P<0.05). Conclusion It is possible to improve the patient's awareness and adherence to disease and medication after taking the personalized health education program in patients with multidrug-resistant tuberculosis disease clinically. It can get a better satisfaction test result.

Objective To study the frequency of lymphocyte cells in hypertrophic adenoid in children with otitis media with effusion. Methods The study group consisted of 45 children suffering from hypertrophic adenoid coexisting with otitis media with effusion (HA/OME). The control group consisted of 34 children with adenoid hypertrophy(HA),but without otitis media. Adenoids excision was implemented. Cells were stained with fluorescently labeled protein-specific antibody. The lymphocyte of adenoids was determined by flow cytometry. Results Lymphocyte cells(CD3+CD4+CD69+T cells(1.29%),CD3+CD4+HLA-DR+T cells(0.61%),CD3+CD8+CD69+ T cells (1.39%),CD3+CD8+HLA-DR+ T cells (0.09%),CD19+CD69+ B cells (2.07%) and CD19+CD62L+B cells(0.21%)in HA/OME group were higher than in HA group:(CD3+CD4+CD69+T cells(1.94%), CD3+CD4+HLA-DR+T cells(0.84%),CD3+CD8+CD69+T cells(2.08%),CD3+CD8+HLA-DR+T cells(0.18%), CD19+CD69+ B cells (3.98%),CD19+CD62L+ B cells (0.46%). In addition ,there are the higher percentage lymphocyte cells(CD3+CD4+ T cells(8.97%),CD3+CD8+ T cells(4.27%),CD3+CD8+ Fas+ T cells(1.24%) and CD19+Fas+ B cells (3.23%) in HA/OME group than those in HA group:(CD3+CD4+ T cells (6.37%), CD3+CD4+CD69+ T cells (1.29%),CD3+CD4+HLA-DR+ T cells (0.61%) and CD3+CD8+ T cells(3.61%). Conclusions The local immune dysfunction resulting from hypertrophic adenoids may have a relationship with otitis media.

OBJECTIVE:To compare therapeutic efficacy and safety of dexamethasone with different administration routes in the treatment of chronic secretory otitis media. METHODS:A total of 150 patients with chronic secretory otitis media were random-ly divided into control group(75 cases)and observation group(75 cases). Control group was given Dexamethasone sodium phos-phate injection 5 mg by auripuncture under endoscope. Observation group was given Dexamethasone sodium phosphate injection 5 mg via eustachian tube under endoscope. Both groups were given relevant medicine every 2 days,for 3 times in total. Clinical effi-cacies of 2 groups were observed as well as bone conduction threshold under 1,2,4,8 kHz,the levels of water channel protein 1 and water channel protein 4,recurrence,the occurrence of tympanic cavity effusion before and after treatment. The occurrence of ADR was recorded. RESULTS:The total response rate of observation group(93.33%)was significantly higher than that of control group(80.00%);recurrence rate and the incidence of tympanic cavity effusion of observation group were significantly lower than those of control group,with statistical significance(P<0.05). Before treatment,there was no statistical significance in bone con-duction threshold,the levels of water channel protein 1 and water channel protein 4 between 2 groups(P>0.05). After treatment, the levels of bone conduction threshold in 2 groups were significantly lower than before;the observation group was significantly lower than the control group. The levels of water channel protein 1 and water channel protein 4 in 2 groups were significantly high-er than before;the observation group was significantly higher than control group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:The administration of dexa-methasone via eustachian tube under endoscope is significantly better than auripuncture in terms of therapeutic efficacy for chronic secretory otitis media,relieving clinical symptom,improving bone conduction threshold,up-regulating water channel protein level, reducing the risk of recurrence and tympanic cavity effusion. The two routes of adimistration have similar safety.

Congenital hydronephrosis is the main cause of childhood chronic renal disease and renal failure,which is also one of the most common anomalies in pediatric urology division.Although the operation is performed to treat obstruction,the renal function of patients get worse and worse,postoperatively.Up to date,there are lots of controversies in evaluation of hydronephrosis and treatment at prenatal and postnatal.According to recent articles,the recent examinations,evaluation system,follow-up and treatment strategies of hydronephrosis are introduced.

Objective To detect serum levels of 25 ? hydroxyvitamin D (25 [OH] D) and matrix metalloproteinase 9(MMP?9)in patients with chronic spontaneous urticaria(CSU), and to explore their significance. Methods Totally, 56 patients with CSU and 25 healthy controls were enrolled into this study. High?performance liquid chromatography tandem?mass spectrometry(LC?MS/MS)and enzyme?linked immunosorbent assay(ELISA) were performed to measure serum levels of 25(OH)D and MMP?9 respectively in these subjects. Statistical analysis was carried out by two independent samples t?test or Wilcoxon rank sum test for comparisons of the above indexes between the two groups, and by Spearman rank correlation analysis for assessment of relationships between the serum levels of 25(OH)D and MMP?9 as well as between the two indexes and disease severity. Results Of the 56 patients, 19 were diagnosed with mild CSU, 26 with moderate CSU and 11 with severe CSU. Compared with the control group, the patient group showed significantly lower serum levels of 25(OH)D(21.74 ± 6.04 vs. 30.17 ± 2.21μg/L, P<0.05), but significantly higher serum levels of MMP?9(Median[P25-P75]:291.55[166.18-594.46]vs. 138.46[94.27-233.12]μg/L, P<0.05). In the patient group, the serum level of 25(OH)D was negatively correlated with that of MMP?9 (rs=-0.26, P<0.05), but uncorrelated with the severity of CSU(rs=-0.20, P>0.05);the serum level of MMP?9 was positively correlated with the severity of CSU(rs=0.27, P<0.05). Conclusion Vitamin D and MMP?9 may play a role in the occurrence of CSU.

Objective To discuss the safety management of hemodialysis equipment for safe and effective treatment to patients.Methods The management methods were summarized in aspects of the installation,utilization and maintenance of hemodialysis equipment.Results The hemodialysis patients received the safe and effective treatment through the strict management and the standardized system.Conclusion The standardized management can not only reduce the unnecessary failures of the equipment,and prolong the service life,but also increase the utilization rate of the equipment,and provide the best,safest and most satisfactory treatment for patients.

An improved method for the determination of Vitamin D2 in baby food (fortified dried milk) was established by using two steps of high-performance liquid chromatography (HPLC). The sample was directly saponified and extracted with benzene. The extracted unsaponifiable matter was firstly subjected to preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (3:2) as a mobile phase. This first step was for the purpose of clean-up, and a fraction containing Vitamin D2 was collected. The separate fraction was subsequently subjected to analytic HPLC with a Zorbax SIL column (straight-phase type) with isopropanol-hexane (0.8 : 99.2) as a mobile phase. The Vitamin D2 was separated from other contaminants and determined with ultraviolet spectrograph by the peak height estimated. The recovery rate was 84%, standard deviation 12.57 and coefficient of variation 4.57%. The proposed method was rapid, accurate, and suitable for the determination of baby food.

Objective To investigate the clinical characteristics,treatment options and prognosis of duplex kidneys in children.Methods Data were retrospectively collected from all patients who were hospitalized at Shengjing Hospital Affiliated to China Medical University from January 2008 to May 201 5.The surgery to retain moie-ty nephron was performed in 30 cases:1 2 cases underwent non common sheath ureter reimplantation,4 cases of the patients had common sheath ureter reimplantation,1 2 patients underwent transurethral resection of the cyst,1 case of them had pyeloureterostomy,and the other patients underwent lower -pole pyeloplasty.Removal of renal moiety was performed in 52 cases:43 patients underwent upper -pole heminephrectomy,8 patients underwent upper pole hemi-nephrectomy and lower -pole ureter reimplantation,and 1 case of the patients had upper -pole heminephrectomy and lower -pole ureteroplasty.Results A total of 54 cases were followed up,the follow -up rate reached 65.9%,the follow -up time lasting 2 months to 4 years,with an average time of 9.53 months.All clinical symptoms disappeared after remove moiety operation.There were small ureteral cysts but compared with the preoperative condition,the symp-toms reduced significantly in 4 cases.All clinical symptoms disappeared in patients whose nephron was retained after operation,and hydronephrosis was alleviated.De novo 2 degree of vesicoureteral reflux was found in 3 out of 1 2 pa-tients who underwent transurethral puncture of the cyst.Conclusions The clinical manifestations of duplex kidney have some characteristics which include recurrent urinary tract infection,normal intermittent voiding urine dripping, abdominal pain,urethral orifice mass,abdominal mass and aggravation of hydronephrosis.Duplex kidney is often asso-ciated with cardiac malformation and cryptorchidism.The preoperative testicular examination and echocardiography should be performed.The choice of operation depends on the clinical manifestation,auxiliary examination results and comprehensive analysis.The prognosis of duplex kidney is well,but patients need to be further followed up af-ter operation.

Vaccination in children suffering neurological diseases is a troublesome issue that people es-pecially pediatricians are reluctant to mention. Due to worrying about the emergence of adverse reactions and concerning on the heat induced seizure,doctors and parents tend to cancel or do not recommend these children to be vaccinated. Additionally,the heat sensitive seizures,such as febrile seizure( FS) ,generalized epilepsy with fe-brile seizure plus(GEFS+),Dravet syndrome account for the largest proportion of the seizure related disease and epilepsy syndrome. Their pathogenesis has been proved relating to the mutation of SCN gene of the sodium channel. For children with FS,GEFS+,and Dravet syndrome,vaccination may lead to fever,which even may bring about convulsions,but it will not result in worse prognosis.

OBJECTIVE:To classify the herbs for promoting blood circulation to remove blood stasis.METHODS:Database of 33 commonly used herbs for promoting blood circulation to remove blood stasis was established by means of SPSS10.0 software package;and the nature,tastes,meridian attributions and actions of the herbs were collected as variables showing the characteristics of classification,and the names of the herbs served as variables for measurement to proceed Q Cluster in Hierarchical Cluster.RESULTS:When the herbs were classified into 4 groups,the first group included the herbs for promoting blood circulation to restore menstrual flow,the second the herbs for promoting blood circulation to arrest pain and dissipate tumor,the third the herbs of herbs for activating blood circulation to dissipate blood stasis and kill pain,the fourth the herbs for severely activating blood circulation to dissipate blood stasis.When the herbs were classified into 5 groups,the fifth group included herbs for activating blood circulation to cure wound.When the herbs were classified into 6 groups,the third one included the herbs for activating blood circulation to exclude wind and the sixth the herbs for activating blood circulation to stanch blood.CONCLUSION:Fuzzy grouping analysis can be applied to classify herbs of different kinds yet with the same efficacy and to classify the same group of herbs according to their drug actions.