Objective To explore the effect of hydrogen peroxide (H2O2) on the expression of human leukocyte antigen-G ( HLA-G) in placental trophoblasts in pregnant women with pre-eclampsia.Methods Forty pregnant women,delivered through cesarean section in the Department of Obstetrics of and Gynecology,the First Affiliated Hospital of Nanjing Medical University from October 2008 to October 2009,were enrolled,including 20 women with pre-eclampsia and 20 healthy gravidas (control group).Colorimetry and western blot were applied,respectively,to determine the level of H2O2 and the expression of HLA-G protein in placental tissues and the correlation between them were analyzed.After 24 hours of seeding,JEG-3 cells (the HLA-G positive cell line of choriocarcinoma) were divided into two groups:intervention group (exposure to 175 μmol/L H2O2) and control group (without H2O2).Immunofluorescence and western blot were used to investigate the expression of HLA-G protein in JEG-3 cells at 24 hours and 48 hours after incubation.Results (1 )The level of H2O2 in placenta in the pre-eclampsia group was significantly higher than that in control group[(105 ±13) nmol·mg-1·prot-1 vs (62 ± 18) nmol·mg-1 ·prot-1,P < 0.05].(2) The expression of HLA-G protein in placenta of the pre-eclampsia group was reduced by88%compared with that of the control (0.20 ± 0.08 vs 1.67 ± 0.65,P < 0.05).( 3) Negative correlation was found between HLA-G level and H2O2 expression in the placenta in both groups(r =-0.895,P =0.000).(4) Compared with the control group,the expression of HLA-G protein in JEG-3 cells,after 24 hours and 48 hours exposure to H2O2,reduced by 39% and 80%,respectively,(3.21 ±0.33 vs 1.95 ±0.25 and 0.65 ±0.08,P <0.05,respectively) and 67% reduction was detected from 24 hours to 48 hours of H2O2 exposure (P <0.05).Fluorescence microscope observed reduced expression of HLA-G in JEG-E cells in the intervention group at 48 hours compared to the control group (P<0.05).Conclusion High level of H2O2 could down-regulate HLA-G expression in the placental trophoblasts in pre-eclampsia which may be involved in the pathogenesis of pre-eclampsia.

The aim of this paper is to investigate the effects of protocatechuic acid (PCA) on the expression of D2DR, iNOS, and TH in striatum and midbrain of Parkinson's disease (PD) mice induced by MPTP. C57BL brown mice were used as experimental animals and were injected with MPTP (25 mg/kg. d) for 7 d to build PD model mice. PCA (10 mg/kg. d) and Madopar (125 mg/kg. d) had been ip administered to the PD mice for the precaution and treatment at the first and lasted for 7 d. The expression of D2DR, iNOS, and TH in the striatum and midbrain of PD mice induced by MPTP was detected by Western blotting method. The results showed that iNOS expression increased while D2DR and TH expression decreased in the striatum and midbrain of MPTP-induced PD mice. However, PCA significantly decreased the expression of iNOS while obviously increased the expression of D2DR and TH in the striatum and midbrain of MPTP-induced PD mice. PCA has a neuroprotective effect on the PD mice induced by MPTP, and its protective effects on mice brain are achieved at least partly by increasing TH and D2DR expression while decreasing iNOS expression.

Objective: To investigate the changes of alpha adrenergic receptors (α-AR) mRNA expression in heart and mesenteric artery in autonomic dysreflexia (AD) rats after spinal cord transection, so as to explore the possible mechanism of AD. Methods: The spinal cord of rats was exposed and the fourth thoracic spinal cord was transected; 4 weeks later, rats' rectum was stimulated by self-made catheter and those with a mean arterial blood pressure increased by more than 15 mmHg (1 mmHg=0.133 kPa) were chosen as AD group (n=16). Heart and mesenteric arteries along with their branches were harvested. mRNA expression of α1A-, α1B-, α1D-, α2A-, α2B- and α2C-AR was quantified by real time PCR and the result was compared with that in sham-operated group (the fourth thoracic spinal cord was exposed but not transected). Results: Compared with sham-opera ted group, rats in AD group had a lower expression of α1A-AR mRNA (P<0.05) and α1B-AR mRNA (P<0.01) in their heart, but the expression of α1D-AR mRNA had no obvious change. Also, rats in AD group had a higer expression of α1A-, α1D-, α2B-AR mRNA (all P<0.01) and α2C-AR mRNA (P<0.05) in their mesentric artery, but α1B- and α2A-AR mRNA expression had no obvious change. Conclusion: AD may occur in the chronic stage of spinal cord transection in rats. The abnormal expression of α1A- and α1B-AR mRNA in the heart and α1A-, α1D-, α2B- and α2C-AR mRNA in the mesenteric artery may be related to AD.

Based on detailed analysis of the status of and problems in the use and management of implantable medical devices and equipment in clinical departments of hospital, we propose some clinically operable solving strategies and methods. It is important to further regulate the use and management of implantable medical devices for timely and safe use the devices in clinical departments.

To investigate the genotype distributions of PLA1/PLA2 polymorphism in Chinese Han population from Beijing and Hebei Province and to study the correlation between the platelet membrane glycoprotein IIIa polymorphism and coronary heart disease (CHD) or CHD with blood-stasis syndrome.

Objective To study the treatment of bladder neck obstruction. Methods Thirty nine cases of bladder neck obstruction were reviewed from 1990 to 1999. There were 25 males and 14 females. 39 cases were treated with Transurethral resection of the bladder neck. Results Symptoms were disappeared in 37 cases. Conclusions Transurethral resection is effective for bladder neck obstruction.

Objective:To investigate the changes of plasma moderate molecule substance (MMS)level in pediatric patients with congenital heart disease during open heart surgery. Method: Siteen cases were studied. Blood samples were taken before anesthesia, after tracheal intubtion, after sternotomy. and rewarming during cardiopulmonary bypass, to measure the plasma MMS levels with ultraviolet absorption spectrophotometry. Result: As compared with that before anesthesia, the MMS levels after tracheal intubtion,sternotomy,and rewarming during cardiopulmonary bypass were significantly elevated(P

ObjectiveTo compare the correlation and agreement of international normalized ratio (INR) measured by INRatio coagulometer and standard laboratory venous blood measurement in order to judge the clinical application value of measuring INR by INRatio coagulometer.MethodsOne hundred who received warfarin were chosen randomly.Venous blood and finger capillary blood were taken simultaneously for measurement of INR by laboratory coagulation analyzer SYSMEX CA-7000 and INRatio coagulometer respectively.The correlation and agreement of INR results tested by the two methods were analyzed.Results When INR ≤5,there was a good correlation between INRatio coagulometer and laboratory coagulation analyzer SYSMEX CA-7000 (r =0.898,P =0.000).The mean bias of the two methods was 0.36 and INR measured by INRatio coagulometer were higher than those tested by laboratory coagulation analyzer SYSMEX CA-7000.By Bland-Altman,it showed a good agreement for 91.2％ (62/68) of the difference INR located 95％ the limited of agreement when the mean INR 1.60 - 3.50,and a bad agreement for only 50.0％(1/2) of the difference INR located 95％ the limited of agreement when the mean INR ＞ 3.50.ConclusionsIn the therapeutic range,INR measured by INRatio coagulometer shows a good correlation and agreement with INR measured by the established laboratory method.INRatio coagulometer can be used to monitor INR in the patients received oral anticoagulant therapy.

Objective To know about the current status of the work engagement of the clinical nurses,and discuss the relatioaships among hardiness,perceived organizational support and work engagement so as to provide basis for improving the level of the work engagement of the clinical nurses.Methods The work engagement scale,hardiness scale and perceived organizational support scale were used to investigate a total of 630 clinical nurses.Results The general score of work engagement,hardiness and perceived organizational support were showed respectively as following:(52.54±8.08),(70.09±14.44) and(82.41±24.98).Every two items of hardiness,perceived organizational support and work engagement were in significant positive correlation.Regression analysis showed after the variable of demographic was controlled,the hardiness and perceived organizational support respectively had significant positive predictive function on work engagement as respective independent variables.Conclusions Hospital managers should create a good working environment for clinical nurses,measures should be taken from individual and organizational aspects so as to improve the hardiness and perceived organizational support and the work positivity of the clinical nurses,in order to make the nurses more positively devoted to their work.It had important practical significance to improve the quality of their services and stabilize nursing group.

0.05).[Conclusion]The factors causing reductions should be removed arthroscopically.Arthrocopy may be a useful micro-treatment method for DDH.