BACKGROUND:In various clinical treatments of soft tissue defects, vacuum sealing drainage technology can effectively control wound infection, shorten the treatment time, and accelerate wound healing. OBJECTIVE:To further understand the features and characteristics of hydrated ethanol polyethylene foam vacuum sealing drainage treatment, and compare the treatment effects against soft tissue defects with the conventional dressing change treatment. METHODS:A total of 140 cases of large soft tissue defects were divided randomly into two groups. Observation group received hydrated ethanol polyethylene foam vacuum sealing drainage treatment, while control group was treated by conventional dressing change. The degree of pain relief during treatment, the family satisfaction, the survival rate of first skin-grafting, the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, the complication rate and other clinical effectiveness indicators were compared between the two groups. RESULTS AND CONCLUSION: In the observation group, visual analog scale scores decreased 5.76±0.68, in the control group visual analog scale score decreased 2.85±0.53, with significant differences between the two groups (P < 0.05), and family satisfaction degree of the observation group was higher (P < 0.05). The survival rate of first skin-grafting of the observation group was higher than the control group (P < 0.05), the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, and the complication rate were lower than the control group (P < 0.05). The results showed that, compared with conventional dressing change treatment, hydrated ethanol polyethylene foam vacuum sealing drainage has advantages of light pain, short treatment time, low rate of complications, and high efficacy.

Objective To find out the correlation between MMP-13 and clinicopathological parameters of breast cancer and identify clinical significance of MMP-13 overexpression on overall survival of breast cancer.Methods Immunohistochemistry was performed on paraffin-embedded tissue microarray containing 159 tissue dots from breast cancer patients.The intensity and the extent of IHC were scored by pathologists blind to clinicopathological parameters of the specimens.Different expression profiles of MMP-13 in breat cancer tissues and paraneoplastic tissues,and correlation between MMP-13 and breast cancer clinicopathological parameters were analyzed for statistical significance respectively.The impact of MMP-13 overexpression on overall survival of breast cancer.Results MMP13 expression were significantly higher in breast cancer tissues(54.4％) than in their corresponding paraneoplastic tissues(27.5％)(P =0.003).Expression of MMP-13 in breast cancer positively correlated with lymphma node metastasis(r =0.257,P =0.006),clinical TNM classification (r =0.310,P =0.001),HER2 expression (r =0.192,P =0.041).However,no significant correlation were oberserved between MMP-13 expression and tumor size,MMP-13 expression and tumor grade,MMP-13 expression and ER expression,MMP-13 expression and PR expression respectively.Conclusions Overexpress of MMP-13 is more common in breast cancer tissues than in their corresponding paraneoplastic tissues,and is an independent prognosis indicator of breast cancer.

Humans ; Neurofibromatosis 2

Ear, Inner ; surgery ; Humans ; Neuroma, Acoustic ; surgery ; Otologic Surgical Procedures ; methods

Humans ; Neuroma, Acoustic ; surgery

Inservice Training ; Otolaryngology ; education ; Physicians

BACKGROUND: Although cultured technique and purified method of Schwann cells (SCs) are changed successively,purity is still limited. Whether double negative selections can culture high-purified SCs still needs a further study.OBJECTIVE: To investigate the effects of double negative selections on growth and purity of SCs.DESIGN: Controlled animal study.SETTING: Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University,and the Beijing Institution of Otolaryngology.MATERIALS: The experiment was carried out in the Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University and the Beijing Institution of Otolaryngology between May 2006 and July 2006. Twenty new-born Wistar rats of 3 days (without gender limitation) were provided by the Experimental Animal Center of the Chinese Medical Academy.METHODS: The sciatic nerves of rats were digested by 0.2% collagenase. ① Grouping: All the rats were divided into 4 groups with 5 each. The experimental group was depurated by differential adhesion and Ara-c for 30 minutes. SCs in the control group 1 and 2 were managed by differential adhesion and Ara-c for 30 minutes, respectively. Cells in the control group 3 were not purified. ② Evaluation: The cell growth was observed under invert microscope; growth curves were measured with MTT method in the experimental group and control group 3 to observe cell proliferation. In addition, the SCs were identified by immunocytochemistry; the culture purity was calculated (ratio of S100 positive cells in each sight).MAIN OUTCOME MEASURES: ① Growth of SCs; ② immunocytochemistry stain of SCs; ③ growth curve of SCs; ④ purity of SCs.RESULTS: ① Growth of SCs: At 5 days after incubation, the SCs proliferated well. SCs were spindle, bipolar and sometimes tripolar, with obvious nuclei and a little cytoplasm. There was more fibroblast cell in the group 3 than that in experimental group. ② Growth curve: The SCs in experimental group entered into the logarithm multiplication period at the third day. ③ Immunocytochemistry: The positive-stained SCs were brown in its body and process, and arranged like swirl. The FB with spread and anomaly shape was slight blue. ④ The ratio of anti-S100 positive cells was higher in experimental group than that in group 3 (P ＜ 0.01).CONCLUSION: With the double negative selection, high-purified SCs are obtained effectively.

Objective To observe the application effect of comfortable nursing in percutaneous coronary intervention for patients with acute myocardium infarction.Methods 112 patients with acute myocardium infarction receiving the treatment of percutaneous coronary intervention were randomly divided into the observation group and the control group,each group including 56 cases.The control group was given conventional nursing,and the observation group was given comfortable nursing.Hospitalization time,nursing satisfaction degree,psychological status before and after treatment and life quality for patients in two groups were evaluated.Results The nursing satisfaction degree,nursing effect and life quality for 56 patients in the observation group were higher evidently than those in the control group.And the average hospitalization time of the observation group was lower than that of the control group.The comparison between two groups had statistical significance.Conclusions Comfortable nursing in percutaneous coronary intervention for patients with acute myocardium infarction can evidently improve medical quality,and can provide strong guarantee for the life and safety of patients.

OBJECTIVE:To prepare nattokinase immunoliposomes and its lyophilized product and to evaluate its pharmaceutical quality. METHODS: Nattokinse liposomes were prepared by film dispersion method, then the immunoliposomes were prepared by carbodiimide method coupled anti-human fibrin D-dimer monoclone antibody SZ-63 and lyophilized and evaluated about its pharmaceutical quality. RESULTS: Lyophilized immunoliposomes were multilamellar vesicles with its mean diameter, Zeta potentials at 251.6 nm, —34.6 mV, respectively. The lyophilized product of nattokinase immunoliposomes exhibited good appearance and satisfactory redispersibility, with its loss on drying at 14.30% and critical relative humidity at 45.66%. CONCLUSION: Nattokinase immunoliposomes and its lyophilized product have been prepared successfully.

OBJECTIVE:To study the adsorbability of plastic transfusion bottle and infusion set to diazepam. METHODS:With the reference of plastic-bottled 5% glucose injection,HPLC was used to determine the changes of mass concentration of diazepam adding into plastic-bottled 5% glucose injection at different time;disposable plastic infusion set was connected to simulate intrave-nous infusion and determine the changes of mass concentration of diazepam in the liquid effluent at different time. RESULTS:The mass concentration of diazepam in glass infusion bottles group was higher than in plastic transfusion bottles group. The adsorbabili-ties of solutions showed the strongest at the time of 15-20 min. The mass concentration of the effluent liquid from the glass bottle group was about 80% of the original concentration and plastic bottle group was about 67%. As the time went on,the adsorbability was gradually saturated at 60 min and the concentration rose again;the mass concentration of the effluent liquid from the glass bot-tle group returned to 95%of the original concentration at 80 min and the plastic bottle group was about 75%of the original concen-tration. CONCLUSIONS:Both plastic infusion bottle and infusion set have strong adsorbability to diazepam. It is suggested that the dosage of diazepam should be increased by 25% to 33% while plastic-bottled 5% glucose injection is used as solvent for intrave-nous drip.