Objective To investigate the relationship between electrophysiological and morphological properties of neurons in visual cortex of developing rat, speculate the coincided degree between electrophysiological and morphological change and realize the mechanism of normal visual development. Methods Whole cell patch-clamp recording and intracellular staining were used to acquire cellular microelectrode recording in visual cortex from Sprague-Dawley rats (4~28 days old). The histological process was made. Results The differences of electrical feature between pyramidal cells and non-pyramidal cells were significant. The morphological maturity degree is different in developing visual cortex. Conclusion The different function of pyramidal and non- pyramidal cells in local integrition is reflected by their electrical feature in the process of visual development. In critical period of visual development, the coincision degree of the electrophysiological and morphological change in visual cortex is larger than that in the subcortex constructure.

In order to investigate the effects of simvastatin on secretion and mRNA expression of interleukin-6 (IL-6) and adiponectin in 3T3-L1 adipocytes, mouse 3T3-L1 adipocytes were stimulated with lipopolysaccharide (LPS). Production and mRNA expression of IL-6 and adiponectin in 3T3-L1 adipocytes were measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. The results showed that simvastatin could significantly suppress LPS-induced IL-6 production and mRNA expression in adipocytes (P<0.05), but increase the LPS-induced adiponectin secretion and mRNA expression in a dose-dependent manner (P<0.05). It was suggested that simvastatin could exert beneficial effects on prevention of obesity-induced metabolic changes in adipocytes.

As required to the standards of the model curricula and with the years of continuous teaching research and reforming,the clinical biochemistry curriculum has kept developing from the teaching staff,content of course,teaching method and means and the teaching condition etc.,thus forming model curricula with special features and notable results.

Purpose To investigate the clinicopathological changes, immuniohistochemistry and molecular genetics phenotypic charac-teristics of the alveolar soft part sarcoma ( ASPS) . Methods 16 cases of ASPS were studied with clinicopathological, cytochemistry technique and immunohistochemical staining, two cases of ASPS were studied by FISH. Results There were 6 males and 10 females with the age 8~58 years (median age 31. 7 years). The tumors were located at limbs, shoulder and back, tongue, vocal cords, lung, cervix, and ureter. The clinical manifestations of the patients was a slowly growing mass. Histopathologically the tumor showed typical organ-like or acinar-like structure with sinus-like blood vessels and the fibrous septa formation. Sometime the clear or abundant eosino-philic granular cytoplasm of the tumors were obvious. The tumor cells had a crystalline substance formation by PAS staining. The tumor cells were positive for TFE3 and Cathepsin K by immunohistochemical staining. The ASPL-TFE3 gene fusion detection of tumor cells were present. Conclusion ASPS often located on the limbs of young patients. It may misdiagnosed as malignant epithelial tumors, primary or metastatic adenocarcinoma and paraganglioma when the tumor locate on a rare anatomical parts or an organs, such as tongue, vocal cords, cervix, ureter, etc. It is valueable that the typical alveolar-like structure of the tumor and the expression for TFE3 and Cathepsin K for the pathological diagnosis of ASPS. It is an important indicator that the ASPL-TFE3 gene fusion detection by FISH for the tumor.

For the reform and development of clinical laboratory education, based on the previous course reform, Laboratory Medicine College of Chongqing Medical University has put the leading teachers'responsibility system of laboratory medicine course into practice. In the recent 3 years, the course is better organized.The students are more interested in the course and they communicate more with teachers than ever before. The effect of the course is obvious. The leading teachers' responsibility system in laboratory medicine course should be promoted.

The electrical pain perception were determined in healthy Chinese adults and factors were assessed. The anxiety-depression state of one hundred healthy Chinese were evaluated and the general information was recorded. Then electrical pain perception (perception threshold, pain threshold, pain tolerance) were assessed by neuromuscular monitor at the same time. Further study of influence factors such as gender, age, body mass index (BMI), left and right sides, degree of anxiety and depression, etc. were carried out on the pain perception. The results showed that 95% of the reference range of electrical perception threshold, pain threshold, pain tolerance were 4-7mA, 7 26mA and 15-60mA, respectively. The bilateral pain perception in males were significantly higher than that in females. There was no difference of effect between left and right sides. Age was positive related to pain perception, degree of depression was negative related to pain perception. BMI, degree of anxiety did not show association with pain perception. Therefore, gender, age and degree of depression were significantly related to electrical pain perception in healthy Chinese adults.
Adult ; Anxiety ; Body Mass Index ; Depression ; physiopathology ; Electric Stimulation ; Female ; Humans ; Male ; Pain Perception ; Pain Threshold ; Reference Values

In order to investigate the effects of simvastatin on secretion and mRNA expression of interleukin-6 (IL-6) and adiponectin in 3T3-L1 adipocytes, mouse 3T3-L1 adipocytes were stimulated with lipopolysaccharide (LPS). Production and mRNA expression of IL-6 and adiponectin in 3T3-L1 adipocytes were measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. The results showed that simvastatin could significantly suppress LPS-induced IL-6 production and mRNA expression in adipocytes (P＜0.05), but increase the LPS-induced adiponectin secretion and mRNA expression in a dose-dependent manner (P＜0.05). It was suggested that simvastatin could exert beneficial effects on prevention of obesity-induced metabolic changes in adipocytes.

The effects of E-cadherin-transfected neural stem cells (NSCs) transplantation for spinal cord injury (SCI) in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty plasmid group and E-cadherin overexpression group (n=15 each). The animal SCI model was established by using the modified Allen's method. NSCs were cultured. Rats in NSCs group were subjected to NSCs transplantation. E-cadherin gene eucaryotic expression vector and pcDNA3.1-E-cadherin were respectively transfected into cultured NSCs, serving as empty plasmid group and E-cadherin overexpression group respectively. At 7th day after transplantation, neurological function of all rats was assessed by Tarlov score. After rats were sacrificed in each group, the number of BrdU and Nestin positive cells was counted by immunohistochemistry. Immumofluorescence method was used to detect the expression of neurofilament protein (NF) and glial fibrillary acidic protein (GFAP). As compared with model control group, the Tarlov score and the number of of BrdU and Nestin positive cells, and the expression of NF and GFAP in NSCs group, empty plasmid group, and E-cadherin overexpression group were increased significantly (P<0.05), and those in the E-cadherin overexpression group were increased more significantly than the other transplantation groups (P<0.05). It was suggested that E-cadherin could be conductive to nerve regeneration and repair probably by promoting the proliferation and differentiation of NSCs.

This paper summarizes that to strengthen the construction of national key disciplines Clinical Laboratory Diagnostic and build professional post-graduate education platform for innovation,the exploration and practice of reform have been done in Chongqing Medical University,in the teaching ranks and exercise disciplinary research direction to enhance the level of scientific research and laboratory construction.

Objective To investigate the effects and mechanisms of electroacupuncture at Tinggong and Yifeng on the local expression of insulin like growth factor-1 (IGF-1) and glucose metabolism in the auditory cor‐tex of guinea pig .Methods Eighteen Adult pigmented guinea pigs were randomly divided into 3 groups .Guinea pigs in electroacupuncture at auricular points group (EAPG ,n= 7) were electroacupunctured at Tinggong and Yifeng acupoints simultaneously for 15 minutes every day ,and electroacupunctur lasted 7 days .The electroacupuncture at non acupoint group (ENAG ,n=6) was electroacupunctured at non acupoints for the same time .The control group (CG ,n=5) received no treatment .After anesthetized ,guinea pigs were injected 18 F-FDG via dorsocuboidal vein , and scanned by PET/CT .PET image and CT image were processed and reconstructed in the workstation to observe the uptake of imaging agent in the brain of guinea pigs .The maximum SUV values and its ratios between auditory cortex and cerebellum were measured .The expression of IGF-1 mRNA were detected by real-time fluorescence quantitative PCR .The results were analyzed by the one-way ANOVA and P<0 .05 means statistical significance . Results 1 .Changes in glucose energy metabolism in the auditory cortex .The ratios of the maximum SUV value betweenthe auditory cortex and cerebellum were 0 .75 ± 0 .06 ,0 .84 ± 0 .05 and 0 .71 ± 0 .06 for CG ,EAPG and ENAG re‐spectively .There was a significant difference between the CG and EAPG (P=0 .042) ,the EAPG and ENAG (P=0 .009) ,and no significant difference between the CG and ENAG (P=0 .361) .2 .The fold changes in IGF-1 mR‐NA were 1 .34 ± 0 .24 ,2 .03 ± 0 .36 and 0 .92 ± 0 .23 for CG ,EAPG and ENAG respectively .There was a significant increase in IGF-1 mRNA expression in auditory cortex of the EAPG (P=0 .002 ,compared with the CG) ,and a significant difference between the EAPG and ENAG groups (P<0 .001) .Conclusion 1 .Acupuncturing at Tinggong and Yifeng point can improve glucose metabolism in the auditory cortex of guinea pig . 2 . Acupuncturing at Tinggong and Yifeng point can result in increase of the local expression of IGF-1 in the auditory cortex of guinea pig .