Aging ; genetics ; physiology ; Diagnosis, Differential ; Humans ; Hypothalamo-Hypophyseal System ; physiology ; Kidney ; physiology ; Medicine, Chinese Traditional ; Neuroimmunomodulation ; physiology ; Pituitary-Adrenal System ; physiology ; Thymus Gland ; physiology ; Yang Deficiency ; physiopathology

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Severe Acute Respiratory Syndrome ; drug therapy ; prevention & control

Drugs, Chinese Herbal ; Humans ; Medicine, Chinese Traditional

Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.

OBJECTIVE:To establish an RP-HPLC method for determination of the contents of oleinic acid and linolic acid in Brucea javanica oil. METHODS:The derivative fatty acid was obtained from extracting and separating process using ?-bromo-acetophenone as derivative reagent,K2CO3 as base and N,N-dimethylformamide (DMF) as solvent. The sample was separated on Shim-pack VP-ODS column(150 mm?4.6 mm,5 ?m); the detection wavelength was set at 242 nm. The mobile phase consisted of methanol-acetonitrile-water(30∶20∶50). RESULTS:The linear ranges of oleinic acid and linolic acid were 0.025～1.0 mg?mL-1(r=0.999 6) and 0.1 ～ 0.8 mg?mL-1(r=0.999 0),respectively. The average recoveries were 98.54% and 98.50%,respectively,and their RSD were 1.81% and 1.90%,respectively (n=6 each). CONCLUSION:The method is accurate and specific,and it is applicable for the quality control of B.javanica oil.

Objective To study the concentration alteration of serum vascular endothelial growth factor (VEGF) in patients with primary and secondary glomerular diseases (GD), the effect of immune inhibitors on the concentration, and analyze the relationship between VEGF concentration and some clinical indexes. Methods Serum VEGF concentration of GD patients was determined by sandwich ABC-ELISA. Results All of the GD patients had higher level of serum VEGF than the control group. Of the 5 groups divided according to the clinical classification, patients of chronic glomerulonephritis(CGN) group, latent glomerulonephritis(LGN) group, Henoch-Schonlein purpura nephritis(HSPN) group and lupus nephritis(LN) group had higher serum VEGF level than the control group, respectively, with no difference among the above groups. The patients without taking immune inhibitors had higher serum VEGF level than the control group,while no difference was observed between the patients taking immune inhibitors and the control group.Patients with glomerulonephritis had a significantly lower serum VEGF level after receiving immunosuppression aggressive therapy than before. The serum VEGF level of nephrotic syndrome in nephrotic phase was higher than in remission stage. In membranous glomerulonephritis serum VEGF correlated positively with 24-hour proteinuria excretion. The serum VEGF level correlated positively with anti-dsDNA Ab titer and the concentration of ? globulins in LN. Conclusion Cytokine VEGF is involved in the pathogenesis of glomerulonephritis, and in the occurrence and progression of proteinuria. Immunosuppression therapy can inhibit the expression of VEGF. In LN patients, the serum VEGF level is related to LN activity.

Objective To study the best parameters of low dose MSCT in examining the pharyngeal disease.Methods 20 volunteers were scanned by MSCT using 10 mm collimation,10 mm interval,120 kV,200 mA,100 mA,50 mA,25 mA parameters,respectively.All of the data was reformed(collimation 2.5 mm,interval 1.3 mm) on ADW 4.0 workstation.The image quality in different dose were scored respectively by 4 experienced radiolagists. Results The best appropriate parameter was 10 mm collimation,10 mm interval,120 kV,50 mA.Conclusion In examining the pharyngeal disease,the application of low dose(120 kV,50 mA) scan mode not only can assure the image quality but can decrease the exposure to patients.

Purpose To explore the role of scanning laser tomography in the assessment of macular hole surgery. Methods Fifteen eyes of 14 patients with macular holes underwent scanning of their affected macular area using the Heidelberg retina tomograph (HRT). The significance of topographic changes postoperatively were determined in eleven eyes which received vitrectomy surgery. The scan field was set at 15? of the retina and the depth was set to 1.5 mm or 2.0 mm. All the measurements were taken for 3 times and the average value of the 3 measurements was used. Results The average hole area was (0.499?0.34) mm 2 and the maximal depth of the hole was (0.284?0.11) mm. Topographic difference analysis of the eleven eyes showed a significant reduction in the height of the retina after vitrectomy. The maximal depth of the hole was (0.063?0.04) mm postoperatively. Conclusion Scanning laser tomography provides an objective evaluation of the anatomic outcome of the macular hole surgery.

ObjectiveTo assess fine needle aspiration (FNAC) in the early diagnosis for auto immune thyroid disease (AITD) in adolescent.Methods Seventy-one patients, age ranging from 9 to 23 years with thyroid enlargement underwent FNAC. Their smears were classified into 4 types according to the degree of degeneration of adenoidal cell and lymphocyte invasion by HE combining with rapid staining.The results were compared with B ultrasound and serum thyroid auto antibody measurement.ResultsB ultrasound showed hyperthyroidism in 12 cases, inflammation in 53, nodule change in 5, normal in 5. Thyroid function examination demonstrated hyperthyroidism in 22 cases, hypothyroidism in 9 cases, and euthyroidism in 40 cases. Auto thyroid antibody (TGAb,TMAb) were all beyond normal range except in 1 case, and TPOAb elevated in 37 cases. FNAC identified Grave′s disease in 14, Hashimoto′s diseases typeⅠ( HT-Ⅰ) in 35 cases,HT(Ⅱ) in 13,HT(Ⅲ) in 3, and HT(Ⅳ) in 6 cases. ConclusionsFNAC contributes to early diagnosis of AITD in adolescent for its safety, simplicity, rapidity and accuracy.

Objective To explore the relationship among the severity of coronary disease, c-reaction protein (CRP) and mild renal dysfunction. Methods 64 patients underwent coronary angiography were divided into two groups according to their serum creatinine (cr) levels: normal renal function(cr