Objective To evaluate the changes and characteristics of respiratory function and pulmonary function in patients with asbestosis by multi-slice spiral CT imaging.Methods 61 asbestosis patents and 30 healthy subjects were enrolled and divided into normal re spiratory function group,mild injury group and moderate/severe injury group according to forced vital capacity and forced expiratory volume in one second.The lungs were scanned at the end of inspiratory and expiratory phase,and the lung volume,lung density and small airway index were measured.Results There were significant differences in lung volume (inspiratory and expiratory volume,volume difference,and volume ratio) among the normal group,the mild injury group and the moderate/severe injury group (all P＜0.05).There were statistically significant differences in the mean lung density (lung density at the end of inspiratory and expiratory phase,lung density difference,lung density ratio) among the three groups (all P＜0.05).There were significant differences in the small airway indexes (tracheal wall thickness ratio at the end of inspiratory and expiratory phase,bronchial wall area ratio) among the three groups (all P＜0.05).Conclusions CT lung function imaging techniques can be used to assess the degree of respiratory dysfunction in asbestosis patients.With the development of pulmonary respiratory dysfunction,the total lung volume decreased,the amount of lungs gradually increased,emphysema gradually increased,and the thickness of small airway wall and alveolar fibrous hypertrophy gradually increased.

Objective To explore the potential therapeutic targets of radiotherapy in colorectal cancer via bioinformatics analysis of gene expression microarray.Methods The gene expression dataset GSE15781 was downloaded from the GEO database.The differentially expressed genes in radiotherapy treated colorectal cancer samples compared with those without radiotherapy,as well as differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy,were obtained by R programming.The DAVID database was used for the functional enrichment analysis of the differentially expressed genes.Besides,protein-protein interaction (PPI) network of the overlapped genes between the two groups of differentially expressed genes were constructed based on the HPRD database.Results A total of 702 differentially expressed genes were obtained for the radiotherapy treated colorectal cancer samples compared with the ones without radiotherapy.Besides,126 differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy were identified.Biological processes that related to cell adhesion,cell proliferation,as well as inflammatory response were found to be enriched in those differentially expressed genes.Moreover,16 overlaps were found between the two groups of differentially expressed genes,and in the PPI network of the 16 overlapped genes,PTGS2 and SDCBP2,etc,were closely correlated with other genes,which might be the important therapeutic targets of radiotherapy.Conclusions Some potential therapeutic targets of radiotherapy in colorectal cancer can be obtained by analysis of gene expression profiles,which may be very important for the study and treatment.

What the medical moral qualities of medical students in adult’s higher education are has an important relation to that of the medical personnel,for most of them come from basic medical units.Whether to develop their good medical moral qualities or to improve their abilities of cognition,conviction and practice,the final purpose is to arouse their enthusiasm of participating in educational reform.In order to enhance their medical moral characters,the courses of medical ethics need to reform entirely both in content and form.

Cognition ; Language ; Medicine, Chinese Traditional ; Meridians ; Metaphor ; Translations

AlM: To comprehensively evaluate of visual quality after epipolis laser in situ keratomileusis ( EPl-LASlK) in mild to moderate myopia.METHODS: Sixty cases ( 120 eyes ) undergone EPl-LASlK with equivalent diopter ( SE ) were divided into two groups:mild myopia group (60 eyes) and moderate myopia group ( 60 eyes ) . Objective visual acuity, aberration, contrast sensitivity and glare sensitivity were measured before and 1wk; 1, 6mo after operation, and made comparative analysis. RESULTS: Postoperative uncorrected visual acuity ( UCVA ) of two groups were better than that of preoperative(P<0. 05). Postoperative 6mo, there were significant differences on total high RMS between two groups ( P < 0. 05 ). Before operation and 6mo after operation, higher - order aberrations had statistically significant difference between two groups (P<0. 05). After 1wk and 1mo, there were statistical significances at the five frequencies between two groups (P<0. 05). After 6mo, the mild myopia group returned to preoperative levels, however, moderate myopia group in the high frequency region ( 18. 0c/d ) had significant difference with before surgery (P<0. 05). 1wk after surgery, there were statistically significant differences on the 3. 0, 6. 0, 12.0, 18. 0c/d frequency in both groups (all P<0. 05), 1mo after surgery, there were significant difference at 12.0, 18.0c/d frequencies (all P<0.05), after 6mo, both groups returned to preoperative levels, showed no significant difference.CONCLUSlON:Visual quality is poorer in mild myopia patients than that in moderate myopia at early stage after EPl-LASlK. Better visual quality is observed at mid-late stage.

Objective To analyze the changes of energy metabolism-related proteins(enzymes) and transformation of energy metabolism in transplanted heart.Methods The hearts transplanted from Lewis to Wister rats as allografts and from Lewis to Lewis rats as isografts were used to estab- lish Ono model.The changes in proteins were observed by using proteomics technique and compared between allografts and isografts in the left ventricular myocardial tissues 2 and 8 weeks after heart transplantation.Peptide mass fingerprint map was obtained by using matrix-assisted laser desorption/ ionization time of flight mass spectrometry(MALDI-TOF-MS)and the matched proteins achieved by using Matrix Science software.Results Eighty proteins(enzymes)of total 65 differential proteins that regulated energy metabolism were identified in allografted hearts,which correlated to the Krebs cycle and electron transport system involving glycometabolism,fatty acid and branched-chain amino acids oxidation.Conclusion The energy metabolism in allografts heart was transformed:mitochondria function was decreased in Krebs cycle but enhanced in electron transport system and outer mitoehon- drial membrane permeability to ATP,accelerated in glycolysis and fatty acid oxidation,however, branched-chain amino acids oxidation were suppressed.

An indexing system is constructed,based on the implementation of prevention and control technology for chronic non-communicable diseases in community health service institutions,using both literature review and Delphi method,and using the Analytic Hierarchy Process (AHP) to calculate the weight of the indexing system.The system comprises three level-1 indexes,8 level-2 indexes and 33 level-3 indexes.The research proposed a transition from emphasis of therapeutic means to prevention,and emphasis on prevention and control technologies to the high-risk population in the prevention and control technology of chronic non-communicable diseases,along with development of techniques and measures to encourage behavioral changes of the population with chronic diseases.

BACKGROUND: Caspase-3 exists in normal cell in form of zymogen and is capable of stimulating cell apoptosis after activated by apoptosis inducing factors.OBJECTIVE: To observe the activity of caspase-3 in hippocampal cytosolic S-100 and neuronal apoptosis in hippocampal regions, so as to discuss the relationship between hippocampal neuronal apoptosis and caspase3 activity during the whole brain ishcemic-reperfuasional injury.DESIGN: Randomized controlled experiment.SETTING: Emergency Department of Southwest Hospital Affiliated to the Third Military Medical University of Chinese PLA.MATERIALS: This experiment was carried out at Southwest Hospital Affiliated to the Third Military Medical University of Chinese PLA from January to April 1999. Totally 182 male Wistar rats were randomly divided into 3 groups: namely sham operation group of 14 rats, cerebral IR group of 84, rats acetyl-asp-glu-val-asp-aldehyde (AC-DEVD-CHO) treatment group of 84 rats, rats in the latter two groups were then subdivided into IR 8, 24, 48, 72, 120 and 168 hours time points subgroups with 14 rats in each.METHODS: The whole brain ischemia 20 minutes and reperfusional model was established on rats in brain IR group and Ac-DEVD-CHO treatment group, and rats were executed separately at post-reperfusional 8, 24,48, 72, 120 and 168 hours for obtaining hippocampal specimen; rats in sham operation group were only underwent anesthesia and operation without common carotid arterial occlusion and burns of vertebral artery, they were executed at 72 hours after operation and hippocampal specimen was obtained. The quantity of amino-methylcoumarin that was produced from the same mass of specimen within same decomposition time was used to reflect the activity of caspase-3. Brain slices that were obtained from different time points were stained and embedded for observing the hippocampal cell apoptosis under fluorescence microscope at 330-350 nm.MAIN OUTCOME MEASURS: ① The caspase-3 activity in hippocampal S-100 in different post-IR time point groups. ② The hippocampal cell apoptosis in different post-IR time point groups. ③ relationship between caspase-3 activity and neuronal apoptosis in hippocampal regions.RESULTS: Totally 182 rats were enrolled in this experiment, 14 rats got lost, thereby date of 168 rats was entered the result analysis. ① The changes of caspase-3 activity in hippocampal S-100 in different post-IR time point groups: There was no change in sham operation group at postoperative 72 hours. In contrast with cerebral IR group, there were obvious reduction in Ac-DEVD-CHO treatment group at post-reperfusional 24, 48,72, 120 and 168 hours [(1.71±0.03, 1.22±0.03; 2.77±0.09, 1.59±0.7;5.54±0.51, 2.3±0.19, 6.28±1.71, 3.43±0.46; 3.11±1.21, 1.73±0.14) nkat/kg;P ＜ 0.05 or 0.01]. ② The hippocampal cell apoptosis in different post-IR time point groups: Under 400× field of vision, the number of apoptotic cells in sham operation group was 1.2±0.4 cells at postoperative 72 hours.It was lower in Ac-DEVD-CHO treatment group at post-reperfusional 24,48, 72, 120 and 168 hours than cerebral IR group [(6.4±1.7, 2.8±0.8;11.8±1.3, 5.8±1.9; 19.8±3.1, 10.0±1.9; 31.2±5.9, 16.4±2.4; 19.8±2.3, 9.0±2.3)cells/400× field of vision; P ＜ 0.01]. ③ Relationship between caspase-3activity and neuronal apoptosis in hippocampal regions: It was proved of linear correlation in cerebral IR group and Ac-DEVD-CHO treatment group,displaying significantly positive correlation r= 0.935 6 or 0.980 0, P ＜ 0.01).CONCLUSION: Caspase-3 activation is one of the major inducer for hippocampal neuronal apoptosis, playing important role in hippocampus neuronal apoptosis in rats during IR injury.

Objective To investigate the proliferative response of peripheral blood mononuclear cells(PBMC) and its depressant effect on small cell lung cancer(SCLC)cells(H446) in patients with paraneoplastic neurological syndrome(PNS).Methods PBMC of 7 patients with PNS and 6 patients with SCLC were cultured with interleukin(IL)-2 in vitro,then cultured separately or mixed with H446 respectively.The proliferation index(PI,the ratio of cellular score which had proliferated and that had not proliferated) of H446,PBMC,CD4+,CD8+ T cell and the ratio of CD4+,CD8+,CD4+/CD8+ were analyzed with flow cytometry and compared with normal control group.Results Compared with cultured separately,the PI of H446,PBMC,CD4+,CD8+T cell in PBMC of PNS and SCLC groups cultured with H446 were not significantly different.Stimulated by IL-2,the ratio of CD4+ T cell in PNS patients [(76.54 ? 3.96)%]was higher than that in normal control group[(51.75 ? 17.3)%](P

OBJECTIVE To evaluate the efficacy and safety of teicoplanin treatment on complicated Gram-positive urinary tract infection.METHODS The patients′ clinical manifestation,laboratory results,bacterial culture changes after intravascular administration of teicoplanin were observed.Each patient was administered teicoplanin(200 mg once a day for 10-14 days,but 400 mg in the first day).RESULTS The effective rate and the bacterial eradication rate were 90.0% and 86.7%,respectively.All patients experienced relief from clinical infective symptoms and systemic reactions.There were no abnormal laboratory findings and adverse reactions.CONCLUSIONS Teicoplanin is an effective and safe antibiotic for the treatment of patients with complicated urinary tract infection.