Objective To study the ankle-brachial index of the diabetic gangrene. Methods ABI of the sixty cases with type 2 diabetes were measured. The results were divided into four groups: normal, gently abnormal moderate abnormal severely abnormal group. Results 30 cases were normal, occupying 50% (P＞0.05), gently and moderate abnormal attained 5 cases (8.33%) and 4 cases (6.67%) respectively, and the rests 21cases were severely abnormal, occupying 35% (P＞0.05).Conclusion The normal ABI was not always indicating type 2 diabetes without the gangrene or tendency.

Objective To explore the preventive and therapeutic effects of diminazene (DIZE) on pulmonary arterial hypertension (PAH) in rats.Methods Left pulmonary lobectomy combined with injection of monocrotaline was used to establish a rat model of pulmonary arterial hypertension.One hundred adult male Wistar rats were randomly divided into blank control group (group A,n=20),DIZE control group (group B,n=20),PAH model group (group C,n=20),PAH model plus DIZE group (group D,n=20) and PAH model plus DIZE and C-16 group (group E,n=20).Angiotensin-converting enzyme 2 (ACE2),IL-6 and IL-8 levels were determined by fluorescence resonance energy transfer (FRET) and enzyme linked immunosorbent assay (ELISA),and the mean pulmonary arterial pressure (mPAP) and right ventricular hypertrophy index (RVHI) were measured.The wall thickness (WT) and intimal hyperplasia score were calculated,and the pulmonary vascular lesions were analyzed using elastic fiber staining.Results RVHI,ACE2 enzyme activity and WT in the group A were significantly different from those of the groups C,D and E (P<0.05).Those of the group D and E were significantly different (P<0.05).The five groups showed significant differences in the overall analysis of each index (P<0.05).Conclusions Diminazene can increase the ACE2 enzyme activity,and decrease the mPAP,RVHI and WT,while reducing the pulmonary arterial medial hypertrophy,and inhibit intimal hyperplasia of pulmonary arterioles.The results of this study provide an experimental basis for the use of diminazene in the treatment of human pulmonary hypertension.

OBJECTIVETo explore optimal choice of surgical treatment for subtrochanteric fractures in older children.

METHODSA retrospective study of 36 older children with subtrochanteric fractures was performed between January 2010 and January 2012. Among them, 18 patients (11 males and 7 females) aged from 7 to 13 years old with an average of 9.4 were treated with titanic elastic nail (TEN) fixation, 4 cases were Type II A, 3 cases were II B, 2 cases were II C, 4 cases were III A, 3 cases were III B according to Seinsheimer classification. Eighteen patients (10 males and 8 females) aged was from 8 to 13 years with an average of 9.6 were treated with locking compression pine (LCP) fixation, and 3 cases were Type II A, 4 cases were II B, 3 cases were II C, 4 cases were IIIA, 2 cases were III B. Fracture healing time, postoperative complications (including wound infection, failure and breakage of internal fixtion, deformities of angular on the sagittal view, deformities of coxa vara) and recovery of hip joint function were observed and recorded.

RESULTSAll children were followed up from 15 to 36 months with an average of 21. Fracture were all healed, the time ranged from 7 to 16 weeks (mean 9.5). Three cases in TEN group occurred mild deformities of angular on the sagittal view, 3 cases occurred deformities of coxa vara and 2 cases occurred limb shortening; while 1 case occurred mild deformities of angular on the sagittal view, and no deformities of coxa vara and limb shortening occurred in LCP group. No early close of epiphyseal injury, avascular necrosis of femoral head occurred. Clinical efficacy were evaluated by Sanders standard, 14 cases got excellent results, 3 cases were moderate in LCP group, while 9 cases in excellent, 4 in moderate in TEN group. There were no significant differences between two group in recovery of hip joint function and complications.

CONCLUSIONFor the treatment of subtrochanteric fractures in older children,the efficacy of LCP fixation is better than that of TFN fixation, which has advantages of reliable fixation, and less complications.

Adolescent ; Bone Nails ; Child ; Female ; Fracture Fixation, Intramedullary ; instrumentation ; methods ; Hip Fractures ; surgery ; Humans ; Male ; Retrospective Studies ; Treatment Outcome

Objective　To investigate the potentiality of adhesion of S. pn to type Ⅱ pneumocyte (A549) in inducing tyrosine phosphorylation of A549 cell proteins and the function subcomponents involved in. Methods　Labelled S. pn with FITC for the observation of kinetic characters of adhesion in vitro; The tyrosine phosphorylation of A549 cell proteins induced by S. pn was examined by immunohistochemistry and ELISA. Results　The adhesion of S. pn to A549 cells was in a time-and dose-dependent fashion suggesting the specific interaction; Tyrosine phosphorylation induced by adhesion was dose-and time-dependent and was mediated by the surface proteins of S. pn. Conclusion　S. pn adheres to A549 cells and triggers the signal cascade in which the surface proteins of S. pn play a vital role.

Albino rats fed on crops from endemic and non-endemic districts of Ke-shan desease, were used in this experiment, which was studied by 85Zn tracer metabolic method after 8 weeks feeding. Uptake of 85Zn (ZnCl2) by blood, heart, liver, kidneys and brown adipose tissue were determined at 24 and 72 hours after injection of this tracer respectively, and its distribution was thoroughly examined. The results revealed that the uptake of 8aZn was higher in the groups fed on crops from endemic districts, particularly significant in liver, kidneys and brown adipose tissue. This may reflect the lacking or deficiency of zinc in the bodies.The regular differences of 85Zn between two groups were certainly related to the crops produced from endemic or aon-endemic districts. In other words, there should be some differences between crops from endemic and non-endemic areas. Thus more attention should be paid to the factors in endemic crops, which induced lacking or deficiency of zinc in the experimental animal and to its relationship with etiology of Keshan disease.

OBJECTIVE:To study the effects of metformin (MF)on serum sex hormone and leptin contents in ovariectormized female rats. METHODS: 40 SD rats were randomly divided into four groups: control group(normal saline 5 mL? kg-1?d-1,NS group),OVX group(normal saline 5 mL?kg-1?d-1),OVX+low and high dosage MF group(135,270 mg? kg-1?d-1,MFlow and MFhigh group),all group were ig drugs for 30 days on end.The serum sex hormone indexes including E2,P,LH,LSH and leptin content were measured with RIA. RESULTS:Compared with NS group,the serum E2 and P content were significantly lower(P0.05). CONCLUSIONS: MF can significantly improve sex hormone secretion and lower serum content of LEP in OVX rats.

Objective To analyse the imaging characteristics of solitary pulmonary nodules(SPNs) with the spiral CT, and detection clinical values of the definitive diagnosis to SPNs. Methods 32 cases of SPNs were identified by spiral CT, 9 cases of them were examinated by enhancement scanning. The diffrentical imaging features were analyzed. Results 24 cases were malignant in imaging(18 cases were identified), 8 cases were benign in imaging(6 cases were identified). 15 cases of malignant SPNs by clinical identified were smooth at edge; 6 cases of benign SPNs by clinical identified. 5 cases of them were anomaly;4 cases of them were burr;2 cases of them were bronchial sign and vesiclat sign.6 cases of the SPNs were calcified. 6 cases of SPNs(9 cases with contrasting) were benign in imaging, 3 cases of them were malignant in imaging. Conclusion The differential diagnostic of SPNs is difficult by spiral CT, but enhancement scanning and epidemiology combined with spiral CT is very valuable for definitive diagnosis.

ObjectiveTo investigate the expression and localization of midkine (MK) in human hepatocellular carcinoma. MethodsIn situ hybridization and immunohistochemical analysis for MK were performed on samples of both tumor tissues and paratumor tissues from HCC and benign liver tumors. ResultsThe distribution and localization of the MK transcripts′ signals determined by in situ hybridization were similar to those obtained by immunohistochemical analysis. Most HCC tissues showed enhanced positive reaction within cytoplasm to both MK probe and MK immunostaining. There was no significant difference in clinicopathological parameters between MK negative and positive cases of HCC. ConclusionsHuman hepatocellular carcinoma overexpresses MK at the mRNA and protein level.

Chronic Disease ; Heart Failure ; metabolism ; Humans ; Phosphorylation ; Ryanodine Receptor Calcium Release Channel ; metabolism

In October 2015 ,the White House issued a New Strategy for American Innovation which was also concerned with precision medicine initiative , BRAIN initiative and health care .This paper introduces the background , main content and developments of this new strategy ,hoping to facilitate the development of healthcare in China .