The inhibitory and antimutagenic effects of 17 compounds, cysteine (1), cinnamic acid (2), rutin (3), tannic acid (4), germanium dioxide (5), fluro uracil (6), sodium copper chlorophylline (7), B-sitosterol (8), vitamin C (9), coumarin (10), vitamin E (11), L-glutathione (oxidized form) (12), L-glutathione (reduced form) (13), sodium selenile (14), organic germanium (15), L-methioine (16) and proline (17) on the SOS response induced by N-methyl-N'-nitro-N-nitrosoguanidine, niethly muthanesulfonate, benzo (a)pyrine and UV were studied by using SOS chromotest. The results showed that compounds 1~15 revealed inhibitory effects, and compounds 2~8 and 10-11 revealed antimutagenic effects. It was demonstrated that cinnamic acid is the best antimutagen among 17 compounds. Cinnamic acid has not only inhibitory effect but also antimutagenic activity towards a wide variety of mutagens/carcinogens. The modes, specificity and end point of action of antimutagens are discussed.

Objective: To investigate the role of intraoperative ultrasound(IOUS) and appropriate approach in local resection for the central small hepatocellular carcinoma(sHCC) which is deeply situated within the parenchyma and adjacent to the hepatic veins. Methods: under IOUS guidance, local resection was carried out for 8 cirrhotic patients with central sHCC which was deeply located and adjacent to hepatic veins by approach of splitting or unroofing superficial hepatic parenchyma. Results: All tumors were precisely localized by IOUS and excised successfully. Except for injury to central hepatic vein in 1 case, no other injury occurred to the hepatic veins in the remaining 7 cases. There was no postoperative mortality, and the liver function recovered well in all cases postoperatively. Conclusion: Local resection by using IOUS and appropriate approach is applicable for the cirrhotic patients with deeply located central sHCC. IOUS plays an important role in avoidance of injuries to the hepatic veins.

ObjectiveTo study the relationship between GDNF and ChAT Mrna and their potential role in the diabetic cognitive dysfunction by observing the change of the expression of GDNF and ChAT mRNA in brain under different blood glucose levels in STZ induced diabetic rats.MethodsForty male Wistar rats were randomly divided into diabetic group without blood glucose control( DM1 group),diabetic rats treated with insulin group( DM2 group),and normal control group( NC group).Blood glucose and body weight were detected every 4 weeks.Twelve weeks later,the hippocampus were submitted for cryostat sections which were subjected to the reverse transcription-polymerase chain reaction(RT-PCR) for GDNF and ChAT mRNA.ResultsThe expression of GDNF mRNA and ChAT mRNA in the hippocampus have negative correlation with HbAlc level( r =-0.962,-0.974,all P ＜ 0.001 );The GDNF expression had positive correlation with the ChAT mRNA expression( r =0.974,P ＜ 0.001 ).ConclusionLongterm chronic hyperglycation caused the “downregulation” of the GDNF expression in the hippocampal,which could cause the central cholinergic dysfunction symbled with the decrease of ChAT mRNA expression and the decline of learning and cognition ability.

Objective To observe the safety and severity of heat-sensitive moxibustion in treating poisonous snakebites-induced bladder dysfunction of different degrees.Method Twenty-six patients diagnosed with bladder dysfunction due to poisonous snakebites were examined by type-B ultrasonic scanning for residual urine volume and uroflowmetry. The patients were treated with heat-sensitive moxibustion. The residual urine volume and uroflowmetry parameters were compared between different severity degrees of bladder dysfunction before and after treatment.Result The residual urine volumes were changed significantly in the mild group and moderate group after treatment (P<0.01); the residual urine volume showed insignificant change after treatment in the severe group (P>0.05). The maximum urine flow rate (Qmax) was significantly changed in the mild group after the treatment (P<0.05); the Qmax was insignificantly changed in the moderate and severe groups after treatment (P>0.05).Conclusion Heat-sensitive moxibustion is significantly effective in treating mild-moderate bladder dysfunction due to poisonous snakebites, and it’s safe and reliable.

BACKGROUND:Cancer related genes and pathways play a critical role in formation and development of cancer.
OBJECTIVE: To investigate the silencing epidermal fatty acid binding protein 5 (FABP-5) by siRNA interference on biological characteristics of the cervical carcinoma cel line SiHa.
METHODS:Design and synthesis of siRNA interference sequence used to transiently transfect SiHa cels was performed according to FABP-5 mRNA coding sequence. mRNA and protein expressions of FABP-5 were detected by RT-PCR and western blot assay, respectively. Meanwhile, cel cycle, proliferation, invasion and apoptosis were detected by flow cytometry, cel counting bit-8 assay, Boyden chamber assay, and TUNEL staining, respectively.
RESULTS AND CONCLUSION: FABP-5 mRNA and protein expressions, cel growth speed, cel number at S phase (cel cycle was arrested at the G0/G1 phase) and cel invasion were significantly decreased, while the cel apoptosis was significantly increased in FABP-5 siRNA group compared with the negative control and blank control groups. Our findings indicate that the specific silencing FABP-5 gene expression by siRNA interference can inhibit SiHa cervical cel growth and proliferation, but accelerate cel apoptosis. Subject headings: RNA, Smal Interfering; Fatty Acid-Binding Proteins; Uterine Cervical Neoplasms; Cel Proliferation; Apoptosis

This paper aims at evaluating the effectiveness of the “Medical and Pharmaceutical Separation” re-form in Beijing in three years , and put forward suggestions for the reform of public hospitals .Using data obtained from hospital database, we compared changes in the proportion of drug cost , the average drug expenditures per time, medical workload , quality , income and structure .The key findings of this study show that after the reform , the quali-ty of medical services has improved .Compared to one year before the reform , in the third year of reform the outpa-tient and inpatient medicine accounted for decreased to 16.98%and 13.3%, from 25.57%and 36%, respectively;and the outpatient and inpatient patients per capita medical expenses decreased by 21.39%and 34.8%respectively. The health care revenue increased by 68 .1%compared with before reform .Drug revenue fell 14.29%before the re-form.Medical service fee income reached 243,500,000 yuan.Income from medical examination and laboratory tests in-creased by 52.60%.Based on the findings of the present investigation , the Trial“Medical and Pharmaceutical Separa-tion” to control the cost of drugs achieved good results .Therefore ,it would be advantageous in adopting the reform of public hospitals in the next step toward the medical examination separated , adjust the price of medical services ,etc.

Objective To discuss the efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with high-risk human papilloma virus infection (hr-HPV).Methods Totally 100 patients who had chronic cervicitis with hr-HPV were selected and divided into two groups randomly.The patients in control group (49 cases) were given Baofukang.The patients in observation group (51 cases) were given Baofukang combined with clindamycin injection.The efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with hr-HPV was evaluated by efficacy,HPV negative rate,inflammatory factors before and after treatment and adverse reaction during treatment.Results After treatment,the effective rate of observation group was 92.1％,and the effective rate of control group was 75.5％,the effective rate of observation group was higher than that of control group (P ＜ 0.05).After treatment,the HPV negative rate in observation group was 90.2％,the control group was 59.2％,the HPV negative rate of observation group was higher than that of control group (P ＜ 0.05).Before treatment,there was no statistical significance on hs-CRP,IL-6,and TNF-α between two groups.After treatment,the hs-CRP,IL-6,and TNF-α were decreased in two groups (P ＜ 0.05).The hs-CRP,IL-6,and TNF-α of observation group were lower than those of control group (P ＜ 0.05).There was no statistical significance on adverse reaction between two groups.Conclusion Baofukang combined with clindamycin injection had a good therapeutic effect on chronic cervicitis with hr-HPV.It could inhibit HPV and inflammatory factors well.It was safe and worthy of clinical use.

This article reviews the recent achievements in parasitology including new diagnostic techniques,molecular mechanism of parasitic pathogenesis,drug resistance,antigenic variation,parasite genomics and proteomics. The perspective development in the area is also discussed.

Objective To approach the correlation between preoperative detection of serumal carcinoembryonic antigen (CEA ) and carbohydrate antigen 19‐9(CA19‐9) and primary tumor/regional lymph nodes/distant metastasis(TNM) staging ,clinicopatho‐logic staging and clinical features of patients with colorectal cancer (CRC) ,and to explore their clinical significance .Methods The dates of 231 patients with CRC treated in the First Affiliated Hospital of Chongqing Medical University from April 2011 to January 2013 were gathered .Then ,the relationship between detection of CEA and CA19‐9 and TNM staging ,clinicopathologic staging and clinical features of patients with CRC were analyzed . Results The positive rate of CEA in patients with CRC was 51 .5%(119/231) and the positive rate of CA19‐9 in patients with CRC was 46 .3% (107/231) .The positive rates of CEA and CA19‐9 were positively correlated with the TNM staging and clinicopathologic staging of CRC (P<0 .05) .Conclusion The positive rate of CEA and CA19‐9 were associated with the tumor progress and metastasis ,so the detection of CEA and CA19‐9 could be used as one of important serumal marker for clinicopathologic staging of CRC .Also ,detection of CEA and CA19‐9 could be used for prog‐nosis judgment and therapeutic schedule institution .

OBJECTIVE:To compare therapeutic efficacy and safety of dexamethasone with different administration routes in the treatment of chronic secretory otitis media. METHODS:A total of 150 patients with chronic secretory otitis media were random-ly divided into control group(75 cases)and observation group(75 cases). Control group was given Dexamethasone sodium phos-phate injection 5 mg by auripuncture under endoscope. Observation group was given Dexamethasone sodium phosphate injection 5 mg via eustachian tube under endoscope. Both groups were given relevant medicine every 2 days,for 3 times in total. Clinical effi-cacies of 2 groups were observed as well as bone conduction threshold under 1,2,4,8 kHz,the levels of water channel protein 1 and water channel protein 4,recurrence,the occurrence of tympanic cavity effusion before and after treatment. The occurrence of ADR was recorded. RESULTS:The total response rate of observation group(93.33%)was significantly higher than that of control group(80.00%);recurrence rate and the incidence of tympanic cavity effusion of observation group were significantly lower than those of control group,with statistical significance(P<0.05). Before treatment,there was no statistical significance in bone con-duction threshold,the levels of water channel protein 1 and water channel protein 4 between 2 groups(P>0.05). After treatment, the levels of bone conduction threshold in 2 groups were significantly lower than before;the observation group was significantly lower than the control group. The levels of water channel protein 1 and water channel protein 4 in 2 groups were significantly high-er than before;the observation group was significantly higher than control group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:The administration of dexa-methasone via eustachian tube under endoscope is significantly better than auripuncture in terms of therapeutic efficacy for chronic secretory otitis media,relieving clinical symptom,improving bone conduction threshold,up-regulating water channel protein level, reducing the risk of recurrence and tympanic cavity effusion. The two routes of adimistration have similar safety.