The mental health state of the 256 fourth-year medical students of Kunming Medical College was preliminarily analyzed by requiring. The students fill in the inquiry sheets (questionare) in a question-and-answer way. The ages of these 136 male and 120 female students ranged from 19 to 23 years old, and the self-desiged 109 items of the investigation were induced into 8 aspects related to the mental health such as the outlook on life etc. The result showed that these are differences between the mental health state of the senior medical students and the traditional ideas, morality, manners of education etc. Some initial points of view were put toward.

Protracted bacterial bronchitis (PBB) is a common cause of children chronic cough,especially in those aged 1 ～ 6 years.It is defined by the presence of persistent wet cough for longer than 4 weeks,but lacks of symptoms or signs of other alternative diagnosis of chronic cough.It shows a normal or near-normal chest radiograph,a intense neutrophilia in bronchoalveolar lavage fluid(BALF),and a chronic purulent bronchitis under bronchochoscopy,and usually resolves after two-week course of appropriate oral antibiotic (usually amoxicillin-clavulanatc).So far,PBB hasn't been fully recognized by most pediatricians,and often been unrecognised or misdiagnosed as asthma.In this paper,pathogenesis,diagnosis and treatment are reviewed.

Glucosylceramide synthase(GCS) plays an important role in ceramide metabolism and can block apoptosis signal pathway which is mediated by ceramide. Recently, studies have demonstrated that overex-pression of GCS exists in various muhidrug resistant cancer cell lines and is related to drug resistant pheno-type. A possible connection between GCS and P-gp is also suggested. Moreover,the level of GCS can be down-regulated to increase tumor cells sensitivity by chemical inhibitor and RNA interference. Thus, inhibition of ac-tivity and expression of GCS is a new mechanism of reversing muhidrug resistance.

Objective To explore the protective effect of Ligustrazine on pulmonary fibrosis caused by hyperoxia in neonatal rats.Met-hods Eighty Sprague-Dawley(SD)clean neonatal rats,which were less than 12 hours old,were enrolled in this study.The neonatal rats were randomly divided into 4 groups:air-exposed control group(group A);air-exposed plus Ligustrazine-treated group(group B);hype-roxia-exposed control group(group C);hyperoxia-exposed plus Ligustrazine-treated group(group D).The neonatal rats of group B and group D were intraperitoneally injected with Ligustrazine[30 mg/(kg?d)] in solution of normal saline(NS),group A and group C were intraperitoneally given the same dose of NS.On 14th day,10 rats of each group were randomly chosen and then killed,the pulmonary pathological changes were observed by HE staining under light microscope.The area percentages of collagen fibers were measured by Masson staining and imaging analysis.The degree of pulmonary fibrosis was evaluated by the area percentages of collagen fibers and hydroxyproline content.The results were analyzed by SPSS 13.0 software.Results On the 14th day of the exposure,the histopathologic examination demonstrated broadened lung interstitium and reduced alveolar quantity and lung fibrosis were developed in group C;Ligustrazine treatment obviously alle-viated the changes of lung histomorphology.The newborn rats in group C had lower body weight compared that of with group A,B(Pa0.05).Conclusion Early use of Ligustrazine can decrease the severity of the pulmonary fibrosis of neonatal rats exposed to hyperoxia.

With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.
Bioengineering ; Industrial Microbiology ; Pichia

Interleukin-32 is a newly descovered cytokine. In vivo and in vitro studies show that it inhibits the proliferation and induces apoptosis of colon cancer and melanoma by inhibiting NF-κB and signaling and transcription activated factor 3 pathways.In addition,IL-32 suppresses tumor growth in different ways in chronic myelogenous leukemia,sarcoma and prostate cancer.

In order to accelerate the comprehensive reform of clinical education,the Ministry of Education and the Ministry of Health decided to implement the program of excellent doctor education training plan in 2012.On the basis of the plan,taking a certain medical college as an example,on the problems such as lack of teachers,unreasonable age structure,the lack of advanced experimental technology and comprehensive talents and the lack of innovation and enterprising spirit and so on,the author puts forward some proposals from the development of experimental teaching,the teachers' personal and professional growth and the institutional and policy level,such as strengthening the foreign exchange,making learning training plan and personal development planning,establishing the evaluation system of the experiment teaching quality and opening up a variety of learning methods to improve the teachers' comprehensive ability.

To explore the application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis, the DNA template was extracted by hydroxybenzene-chloroform from 44 maternal (7-41 weeks) plasma. The Fetus-derived Y sequence DYZ-1 gene (149bp) was chosen to be amplified by PCR. The fragment was identified in all the plasma of male bearing pregnant women with the diagnostic accordance rate being 100.00%. Two of the 22 female bearing pregnant women had false positive results. Among the 44 pregnant women, the diagnostic accordance rate was 88.89% at early pregnant stage, 100.00% at medium pregnant stage, and 96.55% at late stage respectively. The final accuracy of 95.45% was obtained in all cases. It was concluded that by means of hydroxybenzene-chloroform extraction the authors of this article promoted the concentration and purity of the DNA template, and diagnosed more accurately. The results showed that free fetal DNA in the maternal plasma could be regarded as the gene resource for noninvasive prenatal diagnosis.
DNA/*blood ; DNA Primers ; Fetus ; Maternal-Fetal Exchange ; Pregnancy/*blood ; Prenatal Diagnosis/*methods ; *Sex Determination (Genetics)

The genesis of malignant tumor is proved to be mainly caused by regulatory disturbance of cell cycle,in which p27 protein and cyclin D1 protein are major regulators.Cyclin D1 and p27 are positive andnegative regulator respeetively.Recently,redueed expression of p27 protein and overexpression of cyclinD1 pro-tein are found in various eaacers.It has been demonstrated that p27and cyelinD1 are closely associated with the genesis and development of many cancers.Cell cycle and its regulatory has become the focus in tumor research.

OBJECTIVE:To prepare compound ofloxacin gargle,and to establish a method for its quality control.METHODS:Compound oflorxacin gargle was prepared with tinidazole,ofloxcin,heavy peppermint water,glycerol and puri-fied water.Differential spectrophotometry was employed to determine the content of tinidazole without isolation,and then the content of ofloxcin was calculated by formula on the basis of absorbability additivity principle.RESULTS:The prepared gargle was light green yellow clear liquid.The average recovery rates of tinidazole and ofloxacin were98.92%(RSD=0.35%)and99.18%(RSD=0.54%),respectively.No distinct decrease in content of these two components was found in3month test.CONCLUSION:The established method of preparation is simple,and that of content determination is convenient as well as that preparation is stable,which can be widely used in hospitals at primary level.