Objective:To study the differentiation-inducing effects of all-trans-retinoic acid (ATRA) on HPV16 subgenes-immortalized human endocervical cells (H8 cell) and the mechanism. Methods:After H8 cells were treated with ATRA,the aiti-proliferation of ATRA on them was tested by MTT assay,morphological changes observed by LM and TEM,cell cycle analyzed by FCM,expression of Ki67 tested by immunocytochemistry,the activity of telomerase tested by PCR-ELISA,and expressions of HPV and E6 tested by fluorescence immunocytochemistry. Results:ATRA could inhibit proliferation of H8 cells and induce their benign morphodifferentiation. According to FCM,H8 cells accumulated in G1 phase after being treated with ATRA,and expression of Ki67 and activity of telomerase reduced. Conclusion:ATRA could induce the differentiation of H8 cell line,which might be achieved by inhibiting proliferation,blocking cell cycle,and decreasing activity of telomerase.

Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Aeori Talarinowii was identified by its origin and TLC. High-performance liquid chromatography (HPLC) was used to determine the content of β-asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medici-hal material and the control. The results of HPLC showed that a good iinearity was in the range of 0. 1～0. 6 βg, the regression equation was Y=37158. 2X-589. 5(r=0. 999 7), and the average recovery was 96. 78 % (RSD=0. 95 %). Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.

Three patients with prostatic carcinoma who experienced bilateral orchidectomy 2 to 12 weeks ago were examined, and each patient accepted intramuscular injection of estradiol benzoate (E2B) in dose of 1 mg daily for 7 days. Blood sampe was drawn in an interval of 10 min was done for LH pulse analysis before and after the injection for a period of 7h. The results showed that the mean serum LH level decreased significantly from 177,58?3.20 to 174.70?7.60, 138.30?5.83 to 132.02? 5.77 and 145.88? 3.82 to 142.80? 3.65U / L, respectively (P

Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Acori Talarinowii was identified by its origin and TLC. High-performance liquid chromatography(HPLC) was used to determine the content of ? -asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medicinal material and the control. The results of HPLC showed that a good linearity was in the range of 0.1～ 0.6 ? g, the regression equation was Y=37158.2X-589.5(r=0.999 7), and the average recovery was 96.78 % (RSD = 0.95 % ). Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.

Chronic hyperglycemia is often accompanied by abnormal lipid metabolism, hypertension, low-grade systemic inflammation and oxidative stress.These factors increase the risk of cardiovascular disease(CVD)in type 2 diabetes mellitus(T2DM)patients.Comprehensive treatment of T2DM should emphasize the improvement of abnormal lipid metabolism, prevention of weight gain and reduction of the CVD risk in addition to proper glycemic control.Incretin, as a new hypoglycemic drug, has more advantages in improving glucose and lipid metabolism, finally to reduce cardiovascular risk.Through possible mechanisms including direct influence on liver lipid metabolism, change of fat mobilization and delay of gastric emptying, incretin shows positive influence on the lipid metabolic markers such as low-density lipoprotein-cholesterol, high-density lipoprotein-cholesterol, triglycerides, and total cholesterol, while it improves glucose control.Thus, incretin plays an important role in the comprehensive management of type 2 diabetes mellitus.

By analyzing the status quo of moral construction of current Chinese medical students,the authors make an in-depth analysis and accurate grasp of human nature,establish a scientific concept of morality education,and clarify the objective which aims to develop students into real "socialized individuals" with mental health,personal integrity and all-round development.Four moral methods including vertical process,horizontal extension,virtual network and class building are created to promote the ideological and moral construction for medical students.

Objective:To explore the effect of behavioral inhibition/activation system on internet addiction among college students, and to investigate the moderating role of social support.Method:From May to June in 2019, a questionnaire survey was conducted on 630 college students in Tianjin by internet addiction questionnaire, social support rating scale and behavioral inhibition activation scale.SPSS 24.0 and PROCESS macro program were used to analyze the data and check the moderating effect.Results:(1)The score of internet addiction scale for college students(2.73±0.63) was negatively correlated with the scores of all dimensions of social support(objective support: 11.06±4.19, r=-0.35, P<0.01; subjective support: 22.14±4.26, r=-0.25, P<0.01; degree of support utilization: 8.22±2.26, r=-0.33, P<0.01), positively correlated with the behavioral inhibition(2.58±0.53, r=0.63, P<0.01) and activition system(2.55±0.43, r=0.53, P<0.01). The behavior inhibition/activation system positivly predicted Internet addiction behavior( β=0.48, 0.17, both P<0.01). (2)The three dimensions of social support played a moderating role between behavioral inhibition system, behavioral activation system and college students' Internet addiction problem( β=0.08-0.16, all P<0.05). Among them, the objective support had the greatest regulatory effect, and the degree of support utilization was the second. Conclusion:The behavior inhibition/activation system has an important influence on the Internet addiction behavior of college students, and social support plays a very important moderating role.

Objective To observe the clinical efficacy of acupuncture in treating male idiopathic oligoasthenozoospermia. Method A hundred patients with oligoasthenozoospermia were recruited and randomized into a treatment group and a control group, 50 cases in each group. The treatment group was intervened by acupuncture, while the control group was by placebo acupuncture at non-meridian points. The clinical efficacies and changes of the relevant parameters of sperms after treatment were observed. Result The clinical efficacy of the treatment group was significantly better than that of the control group (P<0.05); in the treatment group, the relevant parameters of sperms were significantly improved after intervention (P<0.05); after intervention, the changes of the parameters in the control group were statistically insignificant (P>0.05); after intervention, the improvements of the relevant parameters of sperms in the treatment group were more significant than that in the control group (P<0.05). Conclusion Acupuncture can effectively improve the sperm density, sperm motility, and sperm viability, and enhance the recovery rate in treating infertility, and it’s easy-to-operate, without significant adverse reactions, safe, and reliable.

Objective To investigate the role of vascular endothelial growth factor (VEGF) and transforming growth factor-α (TGF-α) in the angiogenesis of condyloma acuminatum (CA). Methods Tissue specimens were obtained from the lesions of 30 patients with CA and foreskin of 15 normal human controls. Reverse transcription (RT)-PCR and immunohistochemical method were utilized to measure the protein and mRNA expressions of VEGF and TGF-α in the specimens. Microvessel density (MVD) was determined by staining with anti-CD34 polyclonal antibodies. Results A statistical elevation was observed in the expression of VEGF and TGF-α mRNA as well as MVD in CA specimens compared with the normal control specimens (P < 0.001, 0.001, 0.01). The expressions of VEGF and TGF-α protein were observed in 90% and 86.7% of the CA specimens, and in 40% and 26.7% of the control specimens (both P < 0.01). MVD was positively correlated with the expression of VEGF and TGF-α protein in CA tissues (both P < 0.01). Conclusion The overexpression of VEGF and TGF-α in CA tissue may accelerate angiogenesis in, and participate in the development and progression of, CA.

1. Microsurgical relationships of the arteries at the base of the brain and the trigeminal nerve were studied bilaterally on 30 brains. Twenty seven trigeminal nerves made contact with the arteries 33 times which represents 55 percent of the cases. Six of 27 trigeminal nerves had double contact.2. Of 33 arteries in contact with 27 nerves, seventeen involved the superior cerebellar arteries (SCA) (28.33?5.82%), seven the anterior inferior cerebellar arteries (AICA) (11.67?4.15%) and nine the middle inferior cerebellar arteries (MICA) (15.0?4.61%).3. The outer diameter of the superior cerebellar artery is the largest averaging 1.41?0.20 mm; the anterior inferior cerebellar artery comes next averaging 1.06?0.36 mm and the middle inferior cerebellar artery the smallest, averaging 0.62?0.14 mm.4. 27 trigeminal arteries arising from the upper or middle segement of the basal artery were found on the ventral surface of the trigeminal nerves in 17 brains, and 16 trigeminal branches arising from the AICA. MICA. and PICA were also found at the roots of 16 trigeminal nerves. Anastomoses were noted between the trigeminal arteries and the trigeminal branches in four cases of 16 trigeminal nerves.5. The association between the neurovascular contact and the trigeminal neuralgia are discussed. It is suggested that the neurovascular contact either from dorsal or ventral aspects may be the anatomical cause of the trigeminal neuralgia.6. The neurovascular contact of the trigeminal nerve with the SCA was usually on the dorsal or dorsalmedial aspect of the nerve, but the point of contact with AICA or MICA was mainly on the ventral aspect of the nerve. Besides, there are trigeminal arteries and trigeminal branches at the ventral surface of the nerve. All these topographic observations provide microsurgical information for neurosugeons.